################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII AccIII AflII AgeI Aor51HI AscI AseI AsnI AviII BamHI BciVI BclI BfrI BsaBI BsaWI BseAI BsiWI BspDI BspEI BspHI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII Eco47III EcoO65I EcoT22I FbaI FspI HindIII HpaI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI PvuI RcaI RsrII SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 16 GGTACC G^GTACC AclI 1525 AACGTT AA^CGTT AhdI 2370 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1684 GTGCAC G^TGCAC ApaI 529 GGGCCC GGGCC^C ApaLI 1684 GTGCAC G^TGCAC Asp718I 16 GGTACC G^GTACC AspEI 2370 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2939 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 2206 CCTAGG C^CTAGG BlnI 2206 CCTAGG C^CTAGG BlpI 615 GCTGAGC GC^T[ACGT]AGC Bpu1102I 615 GCTGAGC GC^T[ACGT]AGC BsiEI 686 CGGCCG CG[GA][CT]^CG Bsp1407I 2518 TGTACA T^GTACA BspLU11I 799 ACATGT A^CATGT BsrBI 2483 GAGCGG CCGCTC(-3/-3) BsrDI 905 GCAATG GCAATG(2/0) BsrGI 2518 TGTACA T^GTACA BstAPI 1778 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 615 GCTGAGC GC^T[ACGT]AGC EagI 686 CGGCCG C^GGCCG Eam1105I 2370 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 686 CGGCCG C^GGCCG Eco52I 686 CGGCCG C^GGCCG EcoRI 1204 GAATTC G^AATTC EcoRV 1646 GATATC GAT^ATC FseI 683 GGCCGGCC GGCCGG^CC KpnI 16 GGTACC GGTAC^C KspI 622 CCGCGG CCGC^GG MseI 2046 TTAA T^TAA MslI 336 CACCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 684 GCCGGC GCC^GGC NgoMIV 684 GCCGGC G^CCGGC NspI 799 ACATGT [GA]CATG^[CT] PflFI 2939 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 350 CCAACACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1330 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1525 AACGTT AA^CGTT SacI 1609 GAGCTC GAGCT^C SacII 622 CCGCGG CCGC^GG SmaI 2872 CCCGGG CCC^GGG SspBI 2518 TGTACA T^GTACA StuI 1421 AGGCCT AGG^CCT Tru9I 2046 TTAA T^TAA Tth111I 2939 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 350 CCAACACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 2872 CCCGGG C^CCGGG XmaI 2872 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 981 GTCGAC GT^[AC][GT]AC 2367 GTCGAC AcsI 72 GAATTT [GA]^AATT[CT] 1204 GAATTC AflIII 353 ACACGT A^C[GA][CT]GT 799 ACATGT AocI 950 CCTGAGG CC^T[ACGT]AGG 1943 CCTCAGG ApoI 72 GAATTT [GA]^AATT[CT] 1204 GAATTC Asp700I 284 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 825 GAATGCTTTC AvaI 422 CCCGAG C^[CT]CG[GA]G 2872 CCCGGG BbeI 1843 GGCGCC GGCGC^C 2819 GGCGCC BbrPI 354 CACGTG CAC^GTG 524 CACGTG BcgI 1803 CGATTCTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 161 GCAGGCAGCTCG BsaAI 354 CACGTG [CT]AC^GT[GA] 524 CACGTG BsmBI 1396 GAGACG CGTCTC(1/5) 1897 GAGACG BsoBI 422 CCCGAG C^[CT]CG[GA]G 2872 CCCGGG BsrFI 470 ACCGGC [GA]^CCGG[CT] 684 GCCGGC Bsu36I 950 CCTGAGG CC^T[ACGT]AGG 1943 CCTCAGG Cfr10I 470 ACCGGC [GA]^CCGG[CT] 684 GCCGGC Eco81I 950 CCTGAGG CC^T[ACGT]AGG 1943 CCTCAGG EcoNI 1552 CCTCCACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2558 CCTGACCCAGG HaeII 1843 GGCGCC [GA]GCGC^[CT] 2819 GGCGCC KasI 1843 GGCGCC G^GCGCC 2819 GGCGCC NarI 1843 GGCGCC GG^CGCC 2819 GGCGCC PmaCI 354 CACGTG CAC^GTG 524 CACGTG PmlI 354 CACGTG CAC^GTG 524 CACGTG SalI 981 GTCGAC G^TCGAC 2367 GTCGAC SfaNI 612 GATGC GCATC(5/9) 660 GATGC SfoI 1843 GGCGCC GGC^GCC 2819 GGCGCC XcmI 1220 CCATTCTTGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2280 CCATCTGTAAAATGG XmnI 284 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 825 GAATGCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BglII BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI SmlI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII