##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccIII AflII AgeI Aor51HI
AscI AseI AsnI AviII BamHI
BciVI BclI BfrI BsaBI BsaWI
BseAI BsiWI BspDI BspEI BspHI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
Eco47III EcoO65I EcoT22I FbaI FspI
HindIII HpaI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshBI PvuI RcaI
RsrII SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 16 GGTACC G^GTACC
AclI 1525 AACGTT AA^CGTT
AhdI 2370 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1684 GTGCAC G^TGCAC
ApaI 529 GGGCCC GGGCC^C
ApaLI 1684 GTGCAC G^TGCAC
Asp718I 16 GGTACC G^GTACC
AspEI 2370 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2939 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2206 CCTAGG C^CTAGG
BlnI 2206 CCTAGG C^CTAGG
BlpI 615 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 615 GCTGAGC GC^T[ACGT]AGC
BsiEI 686 CGGCCG CG[GA][CT]^CG
Bsp1407I 2518 TGTACA T^GTACA
BspLU11I 799 ACATGT A^CATGT
BsrBI 2483 GAGCGG CCGCTC(-3/-3)
BsrDI 905 GCAATG GCAATG(2/0)
BsrGI 2518 TGTACA T^GTACA
BstAPI 1778 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 615 GCTGAGC GC^T[ACGT]AGC
EagI 686 CGGCCG C^GGCCG
Eam1105I 2370 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 686 CGGCCG C^GGCCG
Eco52I 686 CGGCCG C^GGCCG
EcoRI 1204 GAATTC G^AATTC
EcoRV 1646 GATATC GAT^ATC
FseI 683 GGCCGGCC GGCCGG^CC
KpnI 16 GGTACC GGTAC^C
KspI 622 CCGCGG CCGC^GG
MseI 2046 TTAA T^TAA
MslI 336 CACCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 684 GCCGGC GCC^GGC
NgoMIV 684 GCCGGC G^CCGGC
NspI 799 ACATGT [GA]CATG^[CT]
PflFI 2939 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 350 CCAACACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1330 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1525 AACGTT AA^CGTT
SacI 1609 GAGCTC GAGCT^C
SacII 622 CCGCGG CCGC^GG
SmaI 2872 CCCGGG CCC^GGG
SspBI 2518 TGTACA T^GTACA
StuI 1421 AGGCCT AGG^CCT
Tru9I 2046 TTAA T^TAA
Tth111I 2939 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 350 CCAACACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2872 CCCGGG C^CCGGG
XmaI 2872 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 981 GTCGAC GT^[AC][GT]AC
2367 GTCGAC
AcsI 72 GAATTT [GA]^AATT[CT]
1204 GAATTC
AflIII 353 ACACGT A^C[GA][CT]GT
799 ACATGT
AocI 950 CCTGAGG CC^T[ACGT]AGG
1943 CCTCAGG
ApoI 72 GAATTT [GA]^AATT[CT]
1204 GAATTC
Asp700I 284 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
825 GAATGCTTTC
AvaI 422 CCCGAG C^[CT]CG[GA]G
2872 CCCGGG
BbeI 1843 GGCGCC GGCGC^C
2819 GGCGCC
BbrPI 354 CACGTG CAC^GTG
524 CACGTG
BcgI 1803 CGATTCTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
161 GCAGGCAGCTCG
BsaAI 354 CACGTG [CT]AC^GT[GA]
524 CACGTG
BsmBI 1396 GAGACG CGTCTC(1/5)
1897 GAGACG
BsoBI 422 CCCGAG C^[CT]CG[GA]G
2872 CCCGGG
BsrFI 470 ACCGGC [GA]^CCGG[CT]
684 GCCGGC
Bsu36I 950 CCTGAGG CC^T[ACGT]AGG
1943 CCTCAGG
Cfr10I 470 ACCGGC [GA]^CCGG[CT]
684 GCCGGC
Eco81I 950 CCTGAGG CC^T[ACGT]AGG
1943 CCTCAGG
EcoNI 1552 CCTCCACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2558 CCTGACCCAGG
HaeII 1843 GGCGCC [GA]GCGC^[CT]
2819 GGCGCC
KasI 1843 GGCGCC G^GCGCC
2819 GGCGCC
NarI 1843 GGCGCC GG^CGCC
2819 GGCGCC
PmaCI 354 CACGTG CAC^GTG
524 CACGTG
PmlI 354 CACGTG CAC^GTG
524 CACGTG
SalI 981 GTCGAC G^TCGAC
2367 GTCGAC
SfaNI 612 GATGC GCATC(5/9)
660 GATGC
SfoI 1843 GGCGCC GGC^GCC
2819 GGCGCC
XcmI 1220 CCATTCTTGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2280 CCATCTGTAAAATGG
XmnI 284 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
825 GAATGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BglII BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
SmlI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII