##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AflII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI Asp718I AspEI AspI AviII
AvrII BalI BanI BbeI BbrPI
BbsI BcgI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsiWI BsmBI BspDI
BspMI BsrBI BsrDI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraII DrdI DsaI Eam1105I Eco47III
Eco81I EcoO109I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII HincII
HindII HpaI KasI KpnI KspI
MamI MluI MluNI MscI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PvuI PvuII RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SphI Sse8387I StuI SwaI
Tth111I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccIII 1182 TCCGGA T^CCGGA
AclI 1939 AACGTT AA^CGTT
AflIII 1882 ACATGT A^C[GA][CT]GT
Alw44I 745 GTGCAC G^TGCAC
ApaLI 745 GTGCAC G^TGCAC
AseI 2364 ATTAAT AT^TAAT
AsnI 2364 ATTAAT AT^TAAT
Asp700I 2177 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 405 CCCGAG C^[CT]CG[GA]G
BamHI 1368 GGATCC G^GATCC
BbvCI 10 GCTGAGG CCTCAGC(-5/-2)
BglII 258 AGATCT A^GATCT
BpmI 1069 CTGGAG CTGGAG(16/14)
BsaWI 1182 TCCGGA [AT]^CCGG[AT]
BseAI 1182 TCCGGA T^CCGGA
BsgI 1911 GTGCAG GTGCAG(16/14)
BsoBI 405 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2589 TGTACA T^GTACA
BspEI 1182 TCCGGA T^CCGGA
BspHI 1325 TCATGA T^CATGA
BspLU11I 1882 ACATGT A^CATGT
BsrFI 6 ACCGGC [GA]^CCGG[CT]
BsrGI 2589 TGTACA T^GTACA
Cfr10I 6 ACCGGC [GA]^CCGG[CT]
DraIII 1171 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 974 CGGCCG [CT]^GGCC[GA]
EagI 974 CGGCCG C^GGCCG
EclXI 974 CGGCCG C^GGCCG
Eco52I 974 CGGCCG C^GGCCG
EcoRI 2340 GAATTC G^AATTC
EcoT14I 475 CCTTGG C^C[AT][AT]GG
FauI 848 GCGGG CCCGC(4/6)
HindIII 618 AAGCTT A^AGCTT
MfeI 442 CAATTG C^AATTG
MroI 1182 TCCGGA T^CCGGA
MunI 442 CAATTG C^AATTG
NdeI 1219 CATATG CA^TATG
NspI 1882 ACATGT [GA]CATG^[CT]
PflMI 1170 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 34 GACGGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2364 ATTAAT AT^TAAT
Psp1406I 1939 AACGTT AA^CGTT
RcaI 1325 TCATGA T^CATGA
SacI 991 GAGCTC GAGCT^C
ScaI 2303 AGTACT AGT^ACT
SpeI 1537 ACTAGT A^CTAGT
SspBI 2589 TGTACA T^GTACA
SspI 345 AATATT AAT^ATT
StyI 475 CCTTGG C^C[AT][AT]GG
Van91I 1170 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2177 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcyI 826 GACGCC G[GA]^CG[CT]C
892 GGCGTC
AspHI 745 GTGCAC G[AT]GC[AT]^C
991 GAGCTC
BcnI 963 CCCGG CC^[GC]GG
977 CCGGG
BsaHI 826 GACGCC G[GA]^CG[CT]C
892 GGCGTC
BseRI 958 CTCCTC GAGGAG(10/8)
2073 CTCCTC
BsiEI 4 CGACCG CG[GA][CT]^CG
974 CGGCCG
BsiHKAI 745 GTGCAC G[AT]GC[AT]^C
991 GAGCTC
BstXI 1143 CCATCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1267 CCAAAGATTTGG
Cac8I 1709 GCGTGC GC[ACGT]^[ACGT]GC
2244 GCTTGC
EcoNI 936 CCTCGTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1359 CCTTTAGAAGG
HgaI 826 GACGC GACGC(5/10)
893 GCGTC
Hin1I 826 GACGCC G[GA]^CG[CT]C
892 GGCGTC
MaeII 743 ACGT A^CGT
1940 ACGT
MspA1I 25 CAGCGG C[AC]G^C[GT]G
1305 CAGCGG
NciI 963 CCCGG CC^[GC]GG
977 CCGGG
XbaI 261 TCTAGA T^CTAGA
909 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaII BanII BbvI
BfaI BmyI BsaI BsaJI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI CfoI Cfr13I DdeI DpnII
DraI EarI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII