##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspI AvrII BbrPI
BcgI BclI BfrI BlnI BsaAI
BsaBI BseAI BsgI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrGI Bst1107I BstAPI BstEII BstPI
BstZ17I ClaI CpoI DraI Eco47III
EcoO65I EcoRV EcoT22I FbaI FseI
HincII HindII HpaI KspI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NsiI PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI ScaI SexAI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 2739 GGTACC G^GTACC
AhdI 1688 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 697 CCTGAGG CC^T[ACGT]AGG
Asp718I 2739 GGTACC G^GTACC
AspEI 1688 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 3261 TGGCCA TGG^CCA
BciVI 2680 GTATCC GTATCC(6/5)
BglII 2569 AGATCT A^GATCT
BsiEI 947 CGGCCG CG[GA][CT]^CG
BspLU11I 3168 ACATGT A^CATGT
BsrDI 1212 CATTGC GCAATG(2/0)
BssSI 1485 CTCGTG CACGAG(-5/-1)
BstBI 9 TTCGAA TT^CGAA
Bsu36I 697 CCTGAGG CC^T[ACGT]AGG
DraIII 2180 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2389 GACCATGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 947 CGGCCG C^GGCCG
Eam1105I 1688 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 947 CGGCCG C^GGCCG
Eco52I 947 CGGCCG C^GGCCG
Eco81I 697 CCTGAGG CC^T[ACGT]AGG
EcoNI 1079 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 374 AAGCTT A^AGCTT
KpnI 2739 GGTACC GGTAC^C
MluNI 3261 TGGCCA TGG^CCA
MscI 3261 TGGCCA TGG^CCA
NspV 9 TTCGAA TT^CGAA
PaeR7I 1410 CTCGAG C^TCGAG
SapI 3094 GCTCTTC GCTCTTC(1/4)
SfuI 9 TTCGAA TT^CGAA
SmaI 151 CCCGGG CCC^GGG
XhoI 1410 CTCGAG C^TCGAG
XmaCI 151 CCCGGG C^CCGGG
XmaI 151 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 2518 GTAC GT^AC
2740 GTAC
AflIII 2802 ACGTGT A^C[GA][CT]GT
3168 ACATGT
Asp700I 2119 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2530 GAAGGAGTTC
AviII 518 TGCGCA TGC^GCA
1392 TGCGCA
BamHI 180 GGATCC G^GATCC
3253 GGATCC
BbvCI 3089 CCTCAGC CCTCAGC(-5/-2)
3043 GCTGAGG
BfaI 568 CTAG C^TAG
3448 CTAG
BssHII 490 GCGCGC G^CGCGC
728 GCGCGC
BstXI 2249 CCACGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2743 CCATGCTGGTGG
EaeI 947 CGGCCG [CT]^GGCC[GA]
3261 TGGCCA
EcoRI 1014 GAATTC G^AATTC
1609 GAATTC
FspI 518 TGCGCA TGC^GCA
1392 TGCGCA
MaeI 568 CTAG C^TAG
3448 CTAG
NaeI 83 GCCGGC GCC^GGC
468 GCCGGC
NgoMIV 83 GCCGGC G^CCGGC
468 GCCGGC
NruI 543 TCGCGA TCG^CGA
886 TCGCGA
NspI 3168 ACATGT [GA]CATG^[CT]
3286 GCATGT
PflMI 1085 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1558 CCAGGCTTTGG
RsaI 2518 GTAC GT^AC
2740 GTAC
SbfI 2278 CCTGCAGG CCTGCA^GG
2365 CCTGCAGG
SfiI 471 GGCCTTCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1430 GGCCCAGTGGGCC
Sse8387I 2278 CCTGCAGG CCTGCA^GG
2365 CCTGCAGG
Van91I 1085 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1558 CCAGGCTTTGG
XcmI 648 CCAGAGAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3388 CCAGGACAGCCGTGG
XmnI 2119 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2530 GAAGGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AcyI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BcnI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII