##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AvrII BbeI
BbrPI BciVI BclI BfrI BlnI
BsaAI BsaBI BsaWI BseAI BsiWI
BspEI BspHI BspLU11I BssHII Bst1107I
BstBI BstZ17I CpoI DraI DraIII
DrdI Eco47III EcoNI EcoRI FbaI
FseI HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MamI
MluI MroI NarI NdeI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI StuI SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AcyI 1373 GACGCC G[GA]^CG[CT]C
AhdI 1578 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1071 CCTAAGG CC^T[ACGT]AGG
ApaI 1229 GGGCCC GGGCC^C
AspEI 1578 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1056 GAGCTC G[AT]GC[AT]^C
AviII 1360 TGCGCA TGC^GCA
BalI 643 TGGCCA TGG^CCA
BanI 2541 GGCACC G^G[CT][GA]CC
BbvCI 2348 CCTCAGC CCTCAGC(-5/-2)
BcgI 2337 CGATTCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1474 AGATCT A^GATCT
BlpI 1518 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1518 GCTGAGC GC^T[ACGT]AGC
BsaHI 1373 GACGCC G[GA]^CG[CT]C
BsiHKAI 1056 GAGCTC G[AT]GC[AT]^C
BsmI 2284 GAATGC GAATGC(1/-1)
Bsp1407I 229 TGTACA T^GTACA
BspDI 1716 ATCGAT AT^CGAT
BsrDI 302 CATTGC GCAATG(2/0)
BsrGI 229 TGTACA T^GTACA
BssSI 2631 CTCGTG CACGAG(-5/-1)
BstEII 2053 GGTGACC G^GT[ACGT]ACC
BstPI 2053 GGTGACC G^GT[ACGT]ACC
Bsu36I 1071 CCTAAGG CC^T[ACGT]AGG
CelII 1518 GCTGAGC GC^T[ACGT]AGC
ClaI 1716 ATCGAT AT^CGAT
DsaI 1292 CCATGG C^C[GA][CT]GG
Eam1105I 1578 GACATAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1071 CCTAAGG CC^T[ACGT]AGG
EcoO65I 2053 GGTGACC G^GT[ACGT]ACC
EcoRV 807 GATATC GAT^ATC
EcoT22I 157 ATGCAT ATGCA^T
FspI 1360 TGCGCA TGC^GCA
HgaI 1373 GACGC GACGC(5/10)
Hin1I 1373 GACGCC G[GA]^CG[CT]C
MfeI 1673 CAATTG C^AATTG
MluNI 643 TGGCCA TGG^CCA
MscI 643 TGGCCA TGG^CCA
MunI 1673 CAATTG C^AATTG
NcoI 1292 CCATGG C^CATGG
NsiI 157 ATGCAT ATGCA^T
NspI 628 GCATGC [GA]CATG^[CT]
PflMI 2172 CCAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 263 AGGACCC [GA]G^G[AT]CC[CT]
SacI 1056 GAGCTC GAGCT^C
SfiI 1437 GGCCGCTTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 294 CTCAAG C^T[CT][GA]AG
SphI 628 GCATGC GCATG^C
SspBI 229 TGTACA T^GTACA
Van91I 2172 CCAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 478 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
2524 CAGTGGCTG
AspI 2256 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2419 GACAGAGTC
AvaII 264 GGACC G^G[AT]CC
704 GGACC
BamHI 556 GGATCC G^GATCC
1947 GGATCC
BglI 519 GCCACTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1438 GCCGCTTCGGC
BsgI 800 CTGCAC GTGCAG(16/14)
2450 GTGCAG
BsiEI 242 CGGCCG CG[GA][CT]^CG
2825 CGGCCG
BspMI 1387 ACCTGC ACCTGC(4/8)
1405 ACCTGC
BsrBI 193 CCGCTC CCGCTC(-3/-3)
438 GAGCGG
BstAPI 84 GCAGCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2312 GCAACCTCTGC
EagI 242 CGGCCG C^GGCCG
2825 CGGCCG
EclXI 242 CGGCCG C^GGCCG
2825 CGGCCG
Eco52I 242 CGGCCG C^GGCCG
2825 CGGCCG
HaeII 61 AGCGCC [GA]GCGC^[CT]
1066 AGCGCC
NaeI 240 GCCGGC GCC^GGC
1780 GCCGGC
NgoMIV 240 GCCGGC G^CCGGC
1780 GCCGGC
PflFI 2256 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2419 GACAGAGTC
SmaI 2439 CCCGGG CCC^GGG
2489 CCCGGG
Tth111I 2256 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2419 GACAGAGTC
XcmI 691 CCACCAACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2172 CCAGTCTTTGGGTGG
XmaCI 2439 CCCGGG C^CCGGG
2489 CCCGGG
XmaI 2439 CCCGGG C^CCGGG
2489 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII