##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
AocI Aor51HI AscI AseI AsnI
AspEI AspI BbeI BbrPI BclI
BsaAI BsaBI BseAI BsiWI BspDI
BspEI BsrFI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I RsrII SacI SacII SalI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 1521 GTCTAC GT^[AC][GT]AC
AccII 1287 CGCG CG^CG
AcyI 1656 GACGCC G[GA]^CG[CT]C
AflII 2886 CTTAAG C^TTAAG
AflIII 2869 ACATGT A^C[GA][CT]GT
Alw44I 1069 GTGCAC G^TGCAC
ApaLI 1069 GTGCAC G^TGCAC
Asp700I 3182 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1214 TGCGCA TGC^GCA
AvrII 507 CCTAGG C^CTAGG
BalI 656 TGGCCA TGG^CCA
BamHI 445 GGATCC G^GATCC
BcgI 1248 CGACTTTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2863 GTATCC GTATCC(6/5)
BfrI 2886 CTTAAG C^TTAAG
BglI 1390 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1163 AGATCT A^GATCT
BlnI 507 CCTAGG C^CTAGG
BsaHI 1656 GACGCC G[GA]^CG[CT]C
BsaI 2017 GGTCTC GGTCTC(1/5)
BsiEI 84 CGATCG CG[GA][CT]^CG
BsmI 1847 GCATTC GAATGC(1/-1)
Bsp1407I 851 TGTACA T^GTACA
BspHI 728 TCATGA T^CATGA
BspLU11I 2869 ACATGT A^CATGT
BsrGI 851 TGTACA T^GTACA
BstUI 1287 CGCG CG^CG
EcoNI 606 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1214 TGCGCA TGC^GCA
Hin1I 1656 GACGCC G[GA]^CG[CT]C
MaeII 1991 ACGT A^CGT
MluNI 656 TGGCCA TGG^CCA
MscI 656 TGGCCA TGG^CCA
MvnI 1287 CGCG CG^CG
NdeI 2724 CATATG CA^TATG
NheI 1108 GCTAGC G^CTAGC
PflMI 1620 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2762 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 84 CGATCG CGAT^CG
RcaI 728 TCATGA T^CATGA
SapI 1281 GCTCTTC GCTCTTC(1/4)
SbfI 1728 CCTGCAGG CCTGCA^GG
Sse8387I 1728 CCTGCAGG CCTGCA^GG
SspBI 851 TGTACA T^GTACA
StuI 510 AGGCCT AGG^CCT
Van91I 1620 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3182 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 1455 GGTACC G^GTACC
3049 GGTACC
ApaI 421 GGGCCC GGGCC^C
797 GGGCCC
Asp718I 1455 GGTACC G^GTACC
3049 GGTACC
BbvCI 311 CCTCAGC CCTCAGC(-5/-2)
1499 CCTCAGC
BlpI 136 GCTCAGC GC^T[ACGT]AGC
2675 GCTAAGC
Bpu1102I 136 GCTCAGC GC^T[ACGT]AGC
2675 GCTAAGC
BsaWI 1030 TCCGGT [AT]^CCGG[AT]
1045 ACCGGA
BsgI 1651 GTGCAG GTGCAG(16/14)
2120 GTGCAG
BsmBI 1992 CGTCTC CGTCTC(1/5)
19 GAGACG
BspMI 1727 ACCTGC ACCTGC(4/8)
2291 GCAGGT
BstEII 39 GGTCACC G^GT[ACGT]ACC
3029 GGTAACC
BstPI 39 GGTCACC G^GT[ACGT]ACC
3029 GGTAACC
CelII 136 GCTCAGC GC^T[ACGT]AGC
2675 GCTAAGC
DraI 2102 TTTAAA TTT^AAA
2647 TTTAAA
EaeI 656 TGGCCA [CT]^GGCC[GA]
923 TGGCCG
EcoO65I 39 GGTCACC G^GT[ACGT]ACC
3029 GGTAACC
HaeII 1588 AGCGCC [GA]GCGC^[CT]
2679 AGCGCC
KpnI 1455 GGTACC GGTAC^C
3049 GGTACC
PpuMI 3004 AGGTCCC [GA]G^G[AT]CC[CT]
3377 GGGTCCC
SmaI 887 CCCGGG CCC^GGG
1024 CCCGGG
XmaCI 887 CCCGGG C^CCGGG
1024 CCCGGG
XmaI 887 CCCGGG C^CCGGG
1024 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII