##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccII AgeI Alw44I ApaLI
AscI Asp700I AspI AviII BalI
BbrPI BcgI BciVI BglI BlpI
Bpu1102I BsgI BsiWI BspDI BspMI
BsrFI BssHII BstBI BstEII BstPI
BstUI CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EcoO65I
FseI FspI HindIII HpaI KspI
MluI MluNI MscI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PflFI PinAI PmaCI
PmeI PmlI PshAI PvuI PvuII
RsrII SacII SalI SapI SbfI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I StuI SwaI Tth111I
XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 67 enzymes) #
###################################
Acc65I 983 GGTACC G^GTACC
AccIII 66 TCCGGA T^CCGGA
AclI 2722 AACGTT AA^CGTT
AflII 3413 CTTAAG C^TTAAG
AhdI 565 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 115 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1227 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1607 AGCGCT AGC^GCT
AseI 3255 ATTAAT AT^TAAT
AsnI 3255 ATTAAT AT^TAAT
Asp718I 983 GGTACC G^GTACC
AspEI 565 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 388 CCTAGG C^CTAGG
BamHI 2566 GGATCC G^GATCC
BbeI 394 GGCGCC GGCGC^C
BbsI 3191 GAAGAC GAAGAC(2/6)
BbvCI 2257 GCTGAGG CCTCAGC(-5/-2)
BcnI 1436 CCCGG CC^[GC]GG
BfrI 3413 CTTAAG C^TTAAG
BlnI 388 CCTAGG C^CTAGG
BpuAI 3191 GAAGAC GAAGAC(2/6)
BsaAI 3058 TACGTG [CT]AC^GT[GA]
BsaWI 66 TCCGGA [AT]^CCGG[AT]
BseAI 66 TCCGGA T^CCGGA
BsiEI 2408 CGGCCG CG[GA][CT]^CG
BsmBI 612 CGTCTC CGTCTC(1/5)
Bsp1407I 3354 TGTACA T^GTACA
BspEI 66 TCCGGA T^CCGGA
BspLU11I 3449 ACATGT A^CATGT
BsrBI 78 GAGCGG CCGCTC(-3/-3)
BsrGI 3354 TGTACA T^GTACA
BssSI 3102 CTCGTG CACGAG(-5/-1)
Bst1107I 1170 GTATAC GTA^TAC
BstXI 1544 CCACAATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1170 GTATAC GTA^TAC
Bsu36I 1227 CCTAAGG CC^T[ACGT]AGG
EaeI 2408 CGGCCG [CT]^GGCC[GA]
EagI 2408 CGGCCG C^GGCCG
Eam1105I 565 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2408 CGGCCG C^GGCCG
Eco47III 1607 AGCGCT AGC^GCT
Eco52I 2408 CGGCCG C^GGCCG
Eco81I 1227 CCTAAGG CC^T[ACGT]AGG
EcoNI 1329 CCTCAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1848 GATATC GAT^ATC
EcoT22I 301 ATGCAT ATGCA^T
FauI 80 GCGGG CCCGC(4/6)
HgaI 2621 GCGTC GACGC(5/10)
KasI 394 GGCGCC G^GCGCC
KpnI 983 GGTACC GGTAC^C
MroI 66 TCCGGA T^CCGGA
NarI 394 GGCGCC GG^CGCC
NciI 1436 CCCGG CC^[GC]GG
NsiI 301 ATGCAT ATGCA^T
PacI 3256 TTAATTAA TTAAT^TAA
PaeR7I 3283 CTCGAG C^TCGAG
PflMI 637 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 116 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 3255 ATTAAT AT^TAAT
Psp1406I 2722 AACGTT AA^CGTT
SexAI 950 ACCAGGT A^CC[AT]GGT
SfoI 394 GGCGCC GGC^GCC
SphI 3045 GCATGC GCATG^C
SspBI 3354 TGTACA T^GTACA
Van91I 637 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1288 CCAGCAGTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3283 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 946 GTAGAC GT^[AC][GT]AC
1170 GTATAC
AcyI 394 GGCGCC G[GA]^CG[CT]C
2620 GGCGTC
ApaI 1325 GGGCCC GGGCC^C
1432 GGGCCC
AvaI 1843 CTCGGG C^[CT]CG[GA]G
3283 CTCGAG
BclI 1859 TGATCA T^GATCA
2180 TGATCA
BfaI 389 CTAG C^TAG
2423 CTAG
BsaBI 1848 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2181 GATCATAATC
BsaHI 394 GGCGCC G[GA]^CG[CT]C
2620 GGCGTC
BsoBI 1843 CTCGGG C^[CT]CG[GA]G
3283 CTCGAG
Cac8I 771 GCAAGC GC[ACGT]^[ACGT]GC
3045 GCATGC
EcoRI 2871 GAATTC G^AATTC
3212 GAATTC
FbaI 1859 TGATCA T^GATCA
2180 TGATCA
HaeII 394 GGCGCC [GA]GCGC^[CT]
1607 AGCGCT
HapII 67 CCGG C^CGG
1437 CCGG
Hin1I 394 GGCGCC G[GA]^CG[CT]C
2620 GGCGTC
HpaII 67 CCGG C^CGG
1437 CCGG
MaeI 389 CTAG C^TAG
2423 CTAG
MamI 1848 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2181 GATCATAATC
MfeI 1679 CAATTG C^AATTG
1775 CAATTG
MspA1I 28 CAGCGG C[AC]G^C[GT]G
39 CCGCTG
MspI 67 CCGG C^CGG
1437 CCGG
MunI 1679 CAATTG C^AATTG
1775 CAATTG
PstI 1637 CTGCAG CTGCA^G
3376 CTGCAG
ScaI 2995 AGTACT AGT^ACT
3329 AGTACT
SspI 5 AATATT AAT^ATT
2848 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaII BanI
BanII BbvI BglII BmyI BpmI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspHI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstYI CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HhaI HinP1I HincII HindII HinfI
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII