##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AvaI AviII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BcnI BglI BlpI Bpu1102I
BpuAI BsaAI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsoBI BspEI BspMI BsrBI
BsrFI BssHII Bst1107I BstAPI BstBI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I CpoI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRV FauI FseI
FspI HaeII HapII HhaI HinP1I
HpaII KasI KpnI KspI MfeI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PstI PvuI RsrII SacI SacII
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I Van91I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AclI 747 AACGTT AA^CGTT
AcyI 2034 GACGCC G[GA]^CG[CT]C
AlwNI 1226 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 606 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2436 CCTAGG C^CTAGG
BanI 2624 GGTGCC G^G[CT][GA]CC
BlnI 2436 CCTAGG C^CTAGG
BpmI 784 CTCCAG CTGGAG(16/14)
BsaHI 2034 GACGCC G[GA]^CG[CT]C
Bsp1407I 1731 TGTACA T^GTACA
BspDI 102 ATCGAT AT^CGAT
BspHI 1717 TCATGA T^CATGA
BsrGI 1731 TGTACA T^GTACA
BssSI 759 CTCGTG CACGAG(-5/-1)
BstEII 924 GGTCACC G^GT[ACGT]ACC
BstPI 924 GGTCACC G^GT[ACGT]ACC
BstXI 1960 CCAGGTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 102 ATCGAT AT^CGAT
EcoO65I 924 GGTCACC G^GT[ACGT]ACC
EcoT14I 2436 CCTAGG C^C[AT][AT]GG
EcoT22I 1369 ATGCAT ATGCA^T
Hin1I 2034 GACGCC G[GA]^CG[CT]C
NsiI 1369 ATGCAT ATGCA^T
PflFI 606 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 747 AACGTT AA^CGTT
RcaI 1717 TCATGA T^CATGA
SalI 10 GTCGAC G^TCGAC
SapI 801 GCTCTTC GCTCTTC(1/4)
ScaI 2361 AGTACT AGT^ACT
SphI 2206 GCATGC GCATG^C
SspBI 1731 TGTACA T^GTACA
StyI 2436 CCTAGG C^C[AT][AT]GG
Tth111I 606 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2876 CCAAATATAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 1939 ACATGT A^C[GA][CT]GT
3631 ACATGT
AlwI 777 GATCC GGATC(4/5)
2054 GGATC
AseI 1574 ATTAAT AT^TAAT
1686 ATTAAT
AsnI 1574 ATTAAT AT^TAAT
1686 ATTAAT
BanII 799 GGGCTC G[GA]GC[CT]^C
1992 GAGCCC
BclI 1102 TGATCA T^GATCA
1140 TGATCA
BsaBI 488 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
642 GATCTTCATC
BsmI 1520 GAATGC GAATGC(1/-1)
3055 GAATGC
BspLU11I 1939 ACATGT A^CATGT
3631 ACATGT
Cac8I 2206 GCATGC GC[ACGT]^[ACGT]GC
2441 GCTTGC
DraII 689 AGGTCCC [GA]G^G[ACGT]CC[CT]
788 AGGTCCC
EcoNI 1002 CCTAGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1474 CCTATATTAGG
EcoO109I 689 AGGTCCC [GA]G^G[ACGT]CC[CT]
788 AGGTCCC
EcoRI 1297 GAATTC G^AATTC
1848 GAATTC
FbaI 1102 TGATCA T^GATCA
1140 TGATCA
HgaI 536 GACGC GACGC(5/10)
2034 GACGC
HindIII 2843 AAGCTT A^AGCTT
2930 AAGCTT
HpaI 2529 GTTAAC GTT^AAC
3009 GTTAAC
MaeII 391 ACGT A^CGT
748 ACGT
MamI 488 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
642 GATCTTCATC
MslI 1606 CATTTTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2493 CATACTTATG
PleI 27 GAGTC GAGTC(4/5)
3826 GAGTC
PpuMI 689 AGGTCCC [GA]G^G[AT]CC[CT]
788 AGGTCCC
PshBI 1574 ATTAAT AT^TAAT
1686 ATTAAT
SfcI 947 CTGTAG C^T[GA][CT]AG
3799 CTGTAG
SpeI 2478 ACTAGT A^CTAGT
3772 ACTAGT
StuI 190 AGGCCT AGG^CCT
1471 AGGCCT
SwaI 1353 ATTTAAAT ATTT^AAAT
3339 ATTTAAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccI AcsI AfaI AflII AluI
ApoI Asp700I AvaII BbvI BfaI
BfrI BglII BmyI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII DraI EarI
EcoRII Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII XmnI