##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI AlwNI Aor51HI ApaI
AscI Asp718I AspEI AviII AvrII
BamHI BbeI BbrPI BcgI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BsrDI BsrFI BssHII BstBI BstYI
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoRI
EcoT22I FseI FspI Hin1I KasI
KpnI KspI MamI MfeI MflI
MluI MroI MspA1I MunI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI PvuII RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI Sse8387I StuI
XhoI XhoII
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 2764 CGCG CG^CG
AflIII 1635 ACATGT A^C[GA][CT]GT
Alw44I 489 GTGCAC G^TGCAC
AocI 2820 CCTGAGG CC^T[ACGT]AGG
ApaLI 489 GTGCAC G^TGCAC
Asp700I 28 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3335 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3269 CCCGGG C^[CT]CG[GA]G
AvaII 383 GGTCC G^G[AT]CC
BanII 1912 GAGCTC G[GA]GC[CT]^C
BbvCI 2937 GCTGAGG CCTCAGC(-5/-2)
BciVI 1972 GGATAC GTATCC(6/5)
BsmBI 3340 CGTCTC CGTCTC(1/5)
BsoBI 3269 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1109 TGTACA T^GTACA
BspHI 2235 TCATGA T^CATGA
BspLU11I 1635 ACATGT A^CATGT
BsrGI 1109 TGTACA T^GTACA
BssSI 3128 CACGAG CACGAG(-5/-1)
Bst1107I 972 GTATAC GTA^TAC
BstAPI 3277 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1100 GGTGACC G^GT[ACGT]ACC
BstPI 1100 GGTGACC G^GT[ACGT]ACC
BstUI 2764 CGCG CG^CG
BstZ17I 972 GTATAC GTA^TAC
Bsu36I 2820 CCTGAGG CC^T[ACGT]AGG
DsaI 745 CCATGG C^C[GA][CT]GG
EarI 350 GAAGAG CTCTTC(1/4)
Eco81I 2820 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1100 GGTGACC G^GT[ACGT]ACC
EcoRV 1672 GATATC GAT^ATC
FauI 3118 GCGGG CCCGC(4/6)
HgaI 82 GCGTC GACGC(5/10)
HindIII 1389 AAGCTT A^AGCTT
HpaI 1894 GTTAAC GTT^AAC
Ksp632I 350 GAAGAG CTCTTC(1/4)
MvnI 2764 CGCG CG^CG
NcoI 745 CCATGG C^CATGG
NheI 2470 GCTAGC G^CTAGC
PflFI 3335 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 809 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2235 TCATGA T^CATGA
SacI 1912 GAGCTC GAGCT^C
ScaI 495 AGTACT AGT^ACT
SmaI 3269 CCCGGG CCC^GGG
SpeI 1399 ACTAGT A^CTAGT
SphI 3216 GCATGC GCATG^C
SspBI 1109 TGTACA T^GTACA
SwaI 1782 ATTTAAAT ATTT^AAAT
Tth111I 3335 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1078 TCTAGA T^CTAGA
XcmI 1559 CCAAACTGTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3269 CCCGGG C^CCGGG
XmaI 3269 CCCGGG C^CCGGG
XmnI 28 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 806 GTAGAC GT^[AC][GT]AC
972 GTATAC
AflII 1841 CTTAAG C^TTAAG
2714 CTTAAG
BbsI 433 GAAGAC GAAGAC(2/6)
1004 GAAGAC
BclI 283 TGATCA T^GATCA
1024 TGATCA
BfrI 1841 CTTAAG C^TTAAG
2714 CTTAAG
BpmI 3005 CTCCAG CTGGAG(16/14)
3313 CTCCAG
BpuAI 433 GAAGAC GAAGAC(2/6)
1004 GAAGAC
BseRI 144 GAGGAG GAGGAG(10/8)
1145 GAGGAG
BsmFI 674 GTCCC GGGAC(10/14)
3227 GTCCC
BspMI 1719 ACCTGC ACCTGC(4/8)
1542 GCAGGT
FbaI 283 TGATCA T^GATCA
1024 TGATCA
HaeII 783 GGCGCT [GA]GCGC^[CT]
1322 GGCGCT
HapII 3069 CCGG C^CGG
3270 CCGG
HpaII 3069 CCGG C^CGG
3270 CCGG
MspI 3069 CCGG C^CGG
3270 CCGG
PflMI 662 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2962 CCAGGAGATGG
PstI 1439 CTGCAG CTGCA^G
1540 CTGCAG
SmlI 1841 CTTAAG C^T[CT][GA]AG
2714 CTTAAG
SspI 1742 AATATT AAT^ATT
2421 AATATT
TaqI 1180 TCGA T^CGA
1956 TCGA
TthHB8I 1180 TCGA T^CGA
1956 TCGA
Van91I 662 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2962 CCAGGAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AspHI BalI
BanI BbvI BcnI BfaI BmyI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraI EaeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HhaI HinP1I HincII HindII HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PshBI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI