##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AgeI Alw44I
AocI ApaLI AscI AseI AsnI
Asp718I AvrII BamHI BbrPI BbvCI
BciVI BlnI BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrFI
BsrGI Bst1107I BstAPI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII Eco81I EcoO65I EcoRV
EcoT22I FseI HgaI HincII HindII
HpaI KpnI MamI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RsrII SacI
SalI SapI SbfI ScaI SfiI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AclI 2434 AACGTT AA^CGTT
AcyI 1822 GGCGCC G[GA]^CG[CT]C
AflIII 2550 ACACGT A^C[GA][CT]GT
AhdI 1796 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1539 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 79 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1796 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 849 TGCGCA TGC^GCA
BbeI 1822 GGCGCC GGCGC^C
BcgI 449 CGAGCCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 2147 TACGTG [CT]AC^GT[GA]
BsaHI 1822 GGCGCC G[GA]^CG[CT]C
BsmBI 2000 GAGACG CGTCTC(1/5)
BsmI 2499 GAATGC GAATGC(1/-1)
BssHII 1315 GCGCGC G^CGCGC
BstBI 2387 TTCGAA TT^CGAA
DraI 2893 TTTAAA TTT^AAA
DrdI 1372 GACAGGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 441 CGGCCG C^GGCCG
Eam1105I 1796 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 441 CGGCCG C^GGCCG
Eco52I 441 CGGCCG C^GGCCG
EcoRI 1193 GAATTC G^AATTC
FauI 1288 GCGGG CCCGC(4/6)
FspI 849 TGCGCA TGC^GCA
Hin1I 1822 GGCGCC G[GA]^CG[CT]C
HindIII 2787 AAGCTT A^AGCTT
KasI 1822 GGCGCC G^GCGCC
KspI 1942 CCGCGG CCGC^GG
NarI 1822 GGCGCC GG^CGCC
NcoI 1827 CCATGG C^CATGG
NspI 3033 GCATGT [GA]CATG^[CT]
NspV 2387 TTCGAA TT^CGAA
PflMI 2086 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 2434 AACGTT AA^CGTT
SacII 1942 CCGCGG CCGC^GG
SexAI 1065 ACCTGGT A^CC[AT]GGT
SfoI 1822 GGCGCC GGC^GCC
SfuI 2387 TTCGAA TT^CGAA
SspI 2480 AATATT AAT^ATT
Van91I 2086 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1771 TCTAGA T^CTAGA
XmnI 79 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 2446 GTCTAC GT^[AC][GT]AC
2669 GTAGAC
AflII 740 CTTAAG C^TTAAG
1118 CTTAAG
Aor51HI 495 AGCGCT AGC^GCT
597 AGCGCT
AspI 587 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1726 GACTGTGTC
AvaI 2180 CCCGGG C^[CT]CG[GA]G
2854 CCCGGG
BclI 714 TGATCA T^GATCA
2356 TGATCA
BfrI 740 CTTAAG C^TTAAG
1118 CTTAAG
BglI 881 GCCGCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1094 GCCACGCTGGC
BsoBI 2180 CCCGGG C^[CT]CG[GA]G
2854 CCCGGG
BssSI 860 CACGAG CACGAG(-5/-1)
551 CTCGTG
Eco47III 495 AGCGCT AGC^GCT
597 AGCGCT
EcoNI 1003 CCTCAATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1447 CCTGGGCCAGG
FbaI 714 TGATCA T^GATCA
2356 TGATCA
PflFI 587 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1726 GACTGTGTC
PpuMI 2183 GGGACCT [GA]G^G[AT]CC[CT]
2714 AGGACCT
SmaI 2180 CCCGGG CCC^GGG
2854 CCCGGG
StuI 276 AGGCCT AGG^CCT
2127 AGGCCT
Tth111I 587 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1726 GACTGTGTC
XmaCI 2180 CCCGGG C^CCGGG
2854 CCCGGG
XmaI 2180 CCCGGG C^CCGGG
2854 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApaI ApoI AspHI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BspMI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII