##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbvCI BcgI
BfrI BglI BlnI BlpI Bpu1102I
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BsrBI
BssHII BssSI Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
FseI FspI HincII HindII HpaI
KpnI KspI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NspV PacI PflFI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I StuI SwaI Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 2717 GTCTAC GT^[AC][GT]AC
AcyI 169 GGCGCC G[GA]^CG[CT]C
BbeI 169 GGCGCC GGCGC^C
BclI 507 TGATCA T^GATCA
BcnI 2910 CCCGG CC^[GC]GG
BsaHI 169 GGCGCC G[GA]^CG[CT]C
BseRI 776 GAGGAG GAGGAG(10/8)
BsgI 461 GTGCAG GTGCAG(16/14)
Bsp1407I 445 TGTACA T^GTACA
BspHI 1561 TCATGA T^CATGA
BspMI 746 ACCTGC ACCTGC(4/8)
BsrGI 445 TGTACA T^GTACA
BstAPI 709 GCATGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 578 GGTGACC G^GT[ACGT]ACC
BstPI 578 GGTGACC G^GT[ACGT]ACC
DraIII 2243 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 2557 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 578 GGTGACC G^GT[ACGT]ACC
EcoRV 1850 GATATC GAT^ATC
FauI 27 GCGGG CCCGC(4/6)
FbaI 507 TGATCA T^GATCA
Hin1I 169 GGCGCC G[GA]^CG[CT]C
HindIII 2135 AAGCTT A^AGCTT
KasI 169 GGCGCC G^GCGCC
MspA1I 148 CAGCGG C[AC]G^C[GT]G
NarI 169 GGCGCC GG^CGCC
NciI 2910 CCCGG CC^[GC]GG
NcoI 165 CCATGG C^CATGG
NruI 2830 TCGCGA TCG^CGA
PaeR7I 1170 CTCGAG C^TCGAG
RcaI 1561 TCATGA T^CATGA
SexAI 259 ACCTGGT A^CC[AT]GGT
SfoI 169 GGCGCC GGC^GCC
SphI 664 GCATGC GCATG^C
SspBI 445 TGTACA T^GTACA
SspI 283 AATATT AAT^ATT
XbaI 1900 TCTAGA T^CTAGA
XhoI 1170 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Alw44I 1992 GTGCAC G^TGCAC
2518 GTGCAC
AocI 611 CCTAAGG CC^T[ACGT]AGG
1786 CCTGAGG
ApaLI 1992 GTGCAC G^TGCAC
2518 GTGCAC
Asp700I 2434 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2684 GAATTACTTC
AvaI 524 CCCGAG C^[CT]CG[GA]G
1170 CTCGAG
AvaII 2467 GGACC G^G[AT]CC
2938 GGACC
BbrPI 77 CACGTG CAC^GTG
2243 CACGTG
BbsI 434 GAAGAC GAAGAC(2/6)
1904 GAAGAC
BciVI 1768 GGATAC GTATCC(6/5)
2449 GGATAC
BpuAI 434 GAAGAC GAAGAC(2/6)
1904 GAAGAC
BsmI 2434 GAATGC GAATGC(1/-1)
2438 GCATTC
BsoBI 524 CCCGAG C^[CT]CG[GA]G
1170 CTCGAG
BsrFI 1260 ACCGGC [GA]^CCGG[CT]
2220 GCCGGT
BstXI 1064 CCAGGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2748 CCATCTGCATGG
Bsu36I 611 CCTAAGG CC^T[ACGT]AGG
1786 CCTGAGG
Cfr10I 1260 ACCGGC [GA]^CCGG[CT]
2220 GCCGGT
DsaI 165 CCATGG C^C[GA][CT]GG
2298 CCACGG
Eco81I 611 CCTAAGG CC^T[ACGT]AGG
1786 CCTGAGG
EcoRI 484 GAATTC G^AATTC
2412 GAATTC
HaeII 169 GGCGCC [GA]GCGC^[CT]
1433 GGCGCT
HgaI 2015 GACGC GACGC(5/10)
9 GCGTC
NspI 664 GCATGC [GA]CATG^[CT]
3171 GCATGT
PflMI 1746 CCATAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2242 CCACGTGGTGG
PmaCI 77 CACGTG CAC^GTG
2243 CACGTG
PmlI 77 CACGTG CAC^GTG
2243 CACGTG
Van91I 1746 CCATAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2242 CCACGTGGTGG
XmnI 2434 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2684 GAATTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
BalI BanI BanII BbvI BfaI
BglII BmyI BpmI BsaAI BsaBI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV NsiI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII