##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AvaII AviII AvrII BanII BbeI
BbrPI BbvCI BciVI BcnI BglI
BglII BlnI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
Bsu36I CfoI Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FauI FseI FspI
HaeII HapII HhaI HinP1I HpaII
KasI KspI MamI MfeI MluI
MroI MslI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 580 GGTACC G^GTACC
AcyI 4369 GGCGTC G[GA]^CG[CT]C
Asp718I 580 GGTACC G^GTACC
AspI 3820 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 267 CTCGAG C^[CT]CG[GA]G
BalI 1593 TGGCCA TGG^CCA
BamHI 810 GGATCC G^GATCC
BanI 580 GGTACC G^G[CT][GA]CC
BcgI 497 GCAGCTGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 4369 GGCGTC G[GA]^CG[CT]C
BsaI 3053 GGTCTC GGTCTC(1/5)
BsoBI 267 CTCGAG C^[CT]CG[GA]G
Bst1107I 1305 GTATAC GTA^TAC
BstXI 814 CCAGAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1305 GTATAC GTA^TAC
Cfr13I 1990 GGCCC G^G[ACGT]CC
DraII 1989 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 24 CCATGG C^C[GA][CT]GG
EaeI 1593 TGGCCA [CT]^GGCC[GA]
EcoO109I 1989 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 2230 ATGCAT ATGCA^T
Hin1I 4369 GGCGTC G[GA]^CG[CT]C
HincII 1096 GTTAAC GT[CT]^[GA]AC
HindII 1096 GTTAAC GT[CT]^[GA]AC
HpaI 1096 GTTAAC GTT^AAC
KpnI 580 GGTACC GGTAC^C
MaeII 1903 ACGT A^CGT
MluNI 1593 TGGCCA TGG^CCA
MscI 1593 TGGCCA TGG^CCA
NcoI 24 CCATGG C^CATGG
NsiI 2230 ATGCAT ATGCA^T
PaeR7I 267 CTCGAG C^TCGAG
PflFI 3820 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 746 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1359 CTGCAG CTGCA^G
Sau96I 1990 GGCCC G^G[ACGT]CC
SexAI 3912 ACCAGGT A^CC[AT]GGT
StuI 2787 AGGCCT AGG^CCT
Tth111I 3820 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3289 TCTAGA T^CTAGA
XcmI 2434 CCATGAGAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 267 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflII 1934 CTTAAG C^TTAAG
2420 CTTAAG
AspHI 3293 GAGCAC G[AT]GC[AT]^C
3905 GAGCAC
BclI 307 TGATCA T^GATCA
2386 TGATCA
BfrI 1934 CTTAAG C^TTAAG
2420 CTTAAG
BlpI 1460 GCTGAGC GC^T[ACGT]AGC
2375 GCTAAGC
BmyI 3293 GAGCAC G[AGT]GC[ACT]^C
3905 GAGCAC
Bpu1102I 1460 GCTGAGC GC^T[ACGT]AGC
2375 GCTAAGC
BseRI 2312 GAGGAG GAGGAG(10/8)
3275 GAGGAG
BsgI 2016 CTGCAC GTGCAG(16/14)
1976 GTGCAG
BsiHKAI 3293 GAGCAC G[AT]GC[AT]^C
3905 GAGCAC
BsiYI 2697 CCAATACAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3793 CCCAGAAACGG
BslI 2697 CCAATACAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3793 CCCAGAAACGG
Bsp1286I 3293 GAGCAC G[AGT]GC[ACT]^C
3905 GAGCAC
Bsp1407I 2920 TGTACA T^GTACA
4273 TGTACA
BspHI 108 TCATGA T^CATGA
3004 TCATGA
BspMI 406 GCAGGT ACCTGC(4/8)
1732 GCAGGT
BsrDI 603 CATTGC GCAATG(2/0)
1131 GCAATG
BsrGI 2920 TGTACA T^GTACA
4273 TGTACA
BstYI 810 GGATCC [GA]^GATC[CT]
2719 GGATCT
CelII 1460 GCTGAGC GC^T[ACGT]AGC
2375 GCTAAGC
EcoT14I 24 CCATGG C^C[AT][AT]GG
1641 CCTTGG
FbaI 307 TGATCA T^GATCA
2386 TGATCA
HgaI 425 GACGC GACGC(5/10)
4370 GCGTC
MflI 810 GGATCC [GA]^GATC[CT]
2719 GGATCT
NlaIV 580 GGTACC GG[ACGT]^[ACGT]CC
810 GGATCC
PvuII 498 CAGCTG CAG^CTG
2906 CAGCTG
RcaI 108 TCATGA T^CATGA
3004 TCATGA
SspBI 2920 TGTACA T^GTACA
4273 TGTACA
StyI 24 CCATGG C^C[AT][AT]GG
1641 CCTTGG
XhoII 810 GGATCC [GA]^GATC[CT]
2719 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
BbsI BbvI BfaI BpmI BpuAI
BsaJI BsmAI BsmFI BsmI BspLU11I
BsrI BssKI BstF5I BstNI Cac8I
DdeI DpnII DraI EarI EcoRII
Fnu4HI FokI HaeIII HindIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII NspI
PleI RsaI SapI Sau3AI ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmnI