##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BseAI BsiEI BsiHKAI BsiWI Bsp1407I
BspDI BspEI BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoT14I FbaI
FseI FspI HaeII HincII HindII
HpaI KasI KpnI MamI MfeI
MflI MluI MluNI MroI MscI
MunI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI PvuII
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AcyI 16 GGCGTC G[GA]^CG[CT]C
AflII 2293 CTTAAG C^TTAAG
AseI 2215 ATTAAT AT^TAAT
AsnI 2215 ATTAAT AT^TAAT
AvaI 202 CCCGAG C^[CT]CG[GA]G
BanII 8 GAGCCC G[GA]GC[CT]^C
BfrI 2293 CTTAAG C^TTAAG
BglI 165 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 16 GGCGTC G[GA]^CG[CT]C
BsaWI 1122 TCCGGT [AT]^CCGG[AT]
BseRI 484 GAGGAG GAGGAG(10/8)
BsmBI 63 GAGACG CGTCTC(1/5)
BsoBI 202 CCCGAG C^[CT]CG[GA]G
BsrDI 1181 GCAATG GCAATG(2/0)
BssSI 3 CACGAG CACGAG(-5/-1)
CfoI 36 GCGC GCG^C
DpnII 321 GATC ^GATC
EcoNI 453 CCTGGAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 331 GATATC GAT^ATC
HapII 1123 CCGG C^CGG
HgaI 17 GCGTC GACGC(5/10)
HhaI 36 GCGC GCG^C
Hin1I 16 GGCGTC G[GA]^CG[CT]C
HinP1I 36 GCGC G^CGC
HpaII 1123 CCGG C^CGG
HphI 199 TCACC GGTGA(8/7)
MaeII 596 ACGT A^CGT
MboI 321 GATC ^GATC
MspI 1123 CCGG C^CGG
NdeII 321 GATC ^GATC
PflMI 1932 CCACAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2215 ATTAAT AT^TAAT
PstI 1492 CTGCAG CTGCA^G
Sau3AI 321 GATC ^GATC
Tsp45I 435 GTGAC ^GT[GC]AC
Van91I 1932 CCACAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2445 TCTAGA T^CTAGA
XcmI 268 CCATCAGGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 13 CGCG CG^CG
147 CGCG
AcsI 1816 GAATTC [GA]^AATT[CT]
1900 GAATTC
ApoI 1816 GAATTC [GA]^AATT[CT]
1900 GAATTC
BmyI 8 GAGCCC G[AGT]GC[ACT]^C
540 GGGCAC
BsgI 729 GTGCAG GTGCAG(16/14)
1156 GTGCAG
BsmAI 63 GAGAC GTCTC(1/5)
2433 GTCTC
BsmFI 140 GGGAC GGGAC(10/14)
2378 GTCCC
Bsp1286I 8 GAGCCC G[AGT]GC[ACT]^C
540 GGGCAC
BspMI 32 ACCTGC ACCTGC(4/8)
2336 ACCTGC
BstBI 1384 TTCGAA TT^CGAA
1552 TTCGAA
BstUI 13 CGCG CG^CG
147 CGCG
Cac8I 10 GCCCGC GC[ACGT]^[ACGT]GC
1345 GCAAGC
DraIII 1933 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
2358 CACTCCGTG
DsaI 12 CCGCGG C^C[GA][CT]GG
146 CCGCGG
EarI 448 CTCTTC CTCTTC(1/4)
207 GAAGAG
EcoRI 1816 GAATTC G^AATTC
1900 GAATTC
FauI 11 CCCGC CCCGC(4/6)
118 GCGGG
HaeIII 164 GGCC GG^CC
784 GGCC
HindIII 1211 AAGCTT A^AGCTT
1379 AAGCTT
Ksp632I 448 CTCTTC CTCTTC(1/4)
207 GAAGAG
KspI 12 CCGCGG CCGC^GG
146 CCGCGG
MvnI 13 CGCG CG^CG
147 CGCG
NspV 1384 TTCGAA TT^CGAA
1552 TTCGAA
SacII 12 CCGCGG CCGC^GG
146 CCGCGG
SfaNI 257 GATGC GCATC(5/9)
2555 GATGC
SfcI 53 CTGTAG C^T[GA][CT]AG
1492 CTGCAG
SfuI 1384 TTCGAA TT^CGAA
1552 TTCGAA
SmlI 1376 CTCAAG C^T[CT][GA]AG
2293 CTTAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AflIII AluI Asp700I
AvaII BbvI BfaI BpmI BsaJI
BsiYI BslI BsmI BspHI BspLU11I
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DraII EcoO109I EcoRII EcoT22I
Fnu4HI FokI HinfI ItaI MaeI
MaeIII MboII MnlI MseI MslI
MspA1I MvaI MwoI NdeI NlaIII
NlaIV NsiI NspI PleI PpuMI
RcaI RsaI Sau96I ScrFI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XmnI