##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AclI AcsI AflIII
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI Asp718I AspEI AvrII
BbeI BbrPI BcgI BciVI BclI
BglII BlnI BsaAI BsaI BsiEI
BsiWI BspDI BspHI BspLU11I BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KasI KpnI MfeI MluI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI RcaI
RsrII SalI SapI SbfI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tsp509I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1637 GTAGAC GT^[AC][GT]AC
AflII 2468 CTTAAG C^TTAAG
Alw44I 694 GTGCAC G^TGCAC
AocI 545 CCTCAGG CC^T[ACGT]AGG
ApaI 2015 GGGCCC GGGCC^C
ApaLI 694 GTGCAC G^TGCAC
AspI 1725 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2951 TGCGCA TGC^GCA
BamHI 2195 GGATCC G^GATCC
BfaI 2821 CTAG C^TAG
BfrI 2468 CTTAAG C^TTAAG
BsaBI 196 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1671 GAGACG CGTCTC(1/5)
BspMI 329 ACCTGC ACCTGC(4/8)
BsrDI 103 CATTGC GCAATG(2/0)
Bsu36I 545 CCTCAGG CC^T[ACGT]AGG
DraI 3142 TTTAAA TTT^AAA
DraIII 1562 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 545 CCTCAGG CC^T[ACGT]AGG
FspI 2951 TGCGCA TGC^GCA
HaeII 2041 GGCGCT [GA]GCGC^[CT]
KspI 91 CCGCGG CCGC^GG
MaeI 2821 CTAG C^TAG
MaeII 1028 ACGT A^CGT
MamI 196 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflFI 1725 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 91 CCGCGG CCGC^GG
ScaI 1746 AGTACT AGT^ACT
SexAI 561 ACCAGGT A^CC[AT]GGT
SfcI 2806 CTACAG C^T[GA][CT]AG
SfiI 541 GGCCCCTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 3075 AATATT AAT^ATT
Tth111I 1725 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 885 GACGCC G[GA]^CG[CT]C
1478 GACGCC
Asp700I 490 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1105 GAACCACTTC
BbvCI 2844 CCTCAGC CCTCAGC(-5/-2)
2273 GCTGAGG
BsaHI 885 GACGCC G[GA]^CG[CT]C
1478 GACGCC
BsmI 826 GAATGC GAATGC(1/-1)
225 GCATTC
Bsp1407I 528 TGTACA T^GTACA
780 TGTACA
BsrBI 17 GAGCGG CCGCTC(-3/-3)
2295 GAGCGG
BsrFI 1173 GCCGGC [GA]^CCGG[CT]
2269 GCCGGC
BsrGI 528 TGTACA T^GTACA
780 TGTACA
BstEII 565 GGTGACC G^GT[ACGT]ACC
2764 GGTCACC
BstPI 565 GGTGACC G^GT[ACGT]ACC
2764 GGTCACC
BstXI 1866 CCAGCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2151 CCAGCCAGCTGG
Cfr10I 1173 GCCGGC [GA]^CCGG[CT]
2269 GCCGGC
DrdI 1693 GACACCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2756 GACTCGGCGGTC
EarI 490 GAAGAG CTCTTC(1/4)
2336 GAAGAG
EcoO65I 565 GGTGACC G^GT[ACGT]ACC
2764 GGTCACC
Hin1I 885 GACGCC G[GA]^CG[CT]C
1478 GACGCC
Ksp632I 490 GAAGAG CTCTTC(1/4)
2336 GAAGAG
NaeI 1173 GCCGGC GCC^GGC
2269 GCCGGC
NgoMIV 1173 GCCGGC G^CCGGC
2269 GCCGGC
PflMI 499 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2545 CCACGGGCTGG
PleI 2756 GACTC GAGTC(4/5)
1825 GAGTC
PshAI 590 GACTGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1243 GACCTCCGTC
SmlI 1088 CTCAAG C^T[CT][GA]AG
2468 CTTAAG
SspBI 528 TGTACA T^GTACA
780 TGTACA
TaqI 51 TCGA T^CGA
2430 TCGA
TthHB8I 51 TCGA T^CGA
2430 TCGA
Van91I 499 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2545 CCACGGGCTGG
XcmI 1885 CCACTTCACAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2541 CCAACCACGGGCTGG
XmnI 490 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1105 GAACCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspEI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PpuMI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI StuI
StyI TfiI Tru9I TseI Tsp45I
TspRI XhoII