##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccIII AclI AcyI AhdI
Alw44I ApaLI AscI AspEI BbeI
BbrPI BbvCI BcgI BsaHI BseAI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII Bst1107I BstEII BstPI
BstXI BstZ17I ClaI EagI Eam1105I
EclXI Eco52I EcoO65I EcoT22I FseI
Hin1I HpaI KasI MroI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI PacI PaeR7I PmaCI PmeI
PmlI PshAI Psp1406I PvuI SalI
SapI ScaI SfiI SfoI SgrAI
SmaI SnaBI SspBI SwaI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 3464 GGTACC G^GTACC
AccI 1748 GTCTAC GT^[AC][GT]AC
AflIII 1167 ACGCGT A^C[GA][CT]GT
AgeI 41 ACCGGT A^CCGGT
Aor51HI 373 AGCGCT AGC^GCT
Asp700I 3577 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3464 GGTACC G^GTACC
BsaAI 5804 TACGTG [CT]AC^GT[GA]
BsaBI 3516 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrBI 3767 GAGCGG CCGCTC(-3/-3)
BsrFI 41 ACCGGT [GA]^CCGG[CT]
BssSI 285 CACGAG CACGAG(-5/-1)
BstBI 2098 TTCGAA TT^CGAA
Cfr10I 41 ACCGGT [GA]^CCGG[CT]
CpoI 298 CGGTCCG CG^G[AT]CCG
Eco47III 373 AGCGCT AGC^GCT
EcoNI 220 CCTTTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3494 GAATTC G^AATTC
EcoRV 1125 GATATC GAT^ATC
HaeII 373 AGCGCT [GA]GCGC^[CT]
HincII 2868 GTTGAC GT[CT]^[GA]AC
HindII 2868 GTTGAC GT[CT]^[GA]AC
HindIII 1000 AAGCTT A^AGCTT
KpnI 3464 GGTACC GGTAC^C
KspI 625 CCGCGG CCGC^GG
MamI 3516 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2622 CAATTG C^AATTG
MluI 1167 ACGCGT A^CGCGT
MunI 2622 CAATTG C^AATTG
NspV 2098 TTCGAA TT^CGAA
PinAI 41 ACCGGT A^CCGGT
RsrII 298 CGGTCCG CG^G[AT]CCG
SacII 625 CCGCGG CCGC^GG
SbfI 4810 CCTGCAGG CCTGCA^GG
SexAI 3467 ACCAGGT A^CC[AT]GGT
SfuI 2098 TTCGAA TT^CGAA
SpeI 6400 ACTAGT A^CTAGT
Sse8387I 4810 CCTGCAGG CCTGCA^GG
StuI 4902 AGGCCT AGG^CCT
XmnI 3577 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflII 5037 CTTAAG C^TTAAG
5501 CTTAAG
ApaI 3562 GGGCCC GGGCC^C
4090 GGGCCC
AseI 1731 ATTAAT AT^TAAT
5476 ATTAAT
AsnI 1731 ATTAAT AT^TAAT
5476 ATTAAT
AspI 294 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
339 GACCGCGTC
AvrII 3649 CCTAGG C^CTAGG
4728 CCTAGG
BciVI 3348 GTATCC GTATCC(6/5)
5892 GTATCC
BclI 3091 TGATCA T^GATCA
6254 TGATCA
BfrI 5037 CTTAAG C^TTAAG
5501 CTTAAG
BglI 258 GCCGGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1144 GCCAGTTGGGC
BglII 1272 AGATCT A^GATCT
6057 AGATCT
BlnI 3649 CCTAGG C^CTAGG
4728 CCTAGG
BsaWI 41 ACCGGT [AT]^CCGG[AT]
4231 ACCGGA
BsgI 408 GTGCAG GTGCAG(16/14)
5536 GTGCAG
BsiEI 338 CGACCG CG[GA][CT]^CG
3780 CGGTCG
BsmBI 334 GAGACG CGTCTC(1/5)
1164 GAGACG
BsmI 6432 GAATGC GAATGC(1/-1)
2569 GCATTC
BspHI 737 TCATGA T^CATGA
3357 TCATGA
DrdI 336 GACGACCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
545 GACTCCGAAGTC
FbaI 3091 TGATCA T^GATCA
6254 TGATCA
NcoI 268 CCATGG C^CATGG
5256 CCATGG
PflFI 294 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
339 GACCGCGTC
PshBI 1731 ATTAAT AT^TAAT
5476 ATTAAT
PvuII 724 CAGCTG CAG^CTG
6331 CAGCTG
RcaI 737 TCATGA T^CATGA
3357 TCATGA
SspI 2658 AATATT AAT^ATT
5343 AATATT
Tth111I 294 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
339 GACCGCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII AviII BalI BamHI
BanI BanII BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NheI
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII