################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII Acc65I AclI AcyI AgeI AocI Aor51HI AscI Asp718I AspI AviII BamHI BbeI BbrPI BbvCI BcgI BsaBI BsaHI BsiEI BsiWI BspDI BspMI BsrBI BsrFI BssHII BstBI BstEII BstPI Bsu36I Cfr10I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV FseI FspI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SpeI SphI Sse8387I SwaI Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccIII 860 TCCGGA T^CCGGA AflII 417 CTTAAG C^TTAAG AflIII 942 ACATGT A^C[GA][CT]GT AhdI 238 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3776 GTGCAC G^TGCAC ApaI 179 GGGCCC GGGCC^C ApaLI 3776 GTGCAC G^TGCAC AspEI 238 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanII 179 GGGCCC G[GA]GC[CT]^C BclI 1504 TGATCA T^GATCA BfrI 417 CTTAAG C^TTAAG BglI 4392 GCCACTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 4616 GCTCAGC GC^T[ACGT]AGC Bpu1102I 4616 GCTCAGC GC^T[ACGT]AGC BsaAI 1240 TACGTA [CT]AC^GT[GA] BsaWI 860 TCCGGA [AT]^CCGG[AT] BseAI 860 TCCGGA T^CCGGA BsgI 817 CTGCAC GTGCAG(16/14) BsmBI 750 CGTCTC CGTCTC(1/5) BspEI 860 TCCGGA T^CCGGA BspHI 2288 TCATGA T^CATGA BspLU11I 942 ACATGT A^CATGT BssSI 4815 CACGAG CACGAG(-5/-1) Bst1107I 1771 GTATAC GTA^TAC BstAPI 3067 GCATAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 3689 CCAGCAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1771 GTATAC GTA^TAC CelII 4616 GCTCAGC GC^T[ACGT]AGC Eam1105I 238 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 4227 CCTGGTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 1504 TGATCA T^GATCA HaeII 4850 AGCGCC [GA]GCGC^[CT] HgaI 5165 GACGC GACGC(5/10) MroI 860 TCCGGA T^CCGGA RcaI 2288 TCATGA T^CATGA SapI 4846 GAAGAGC GCTCTTC(1/4) SnaBI 1240 TACGTA TAC^GTA StuI 2394 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccI 831 GTCTAC GT^[AC][GT]AC 1771 GTATAC AlwNI 493 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG 3734 CAGAAACTG AvaI 176 CCCGGG C^[CT]CG[GA]G 4448 CCCGGG AvrII 160 CCTAGG C^CTAGG 2421 CCTAGG BalI 3562 TGGCCA TGG^CCA 4350 TGGCCA BanI 3426 GGTGCC G^G[CT][GA]CC 4892 GGCACC BglII 1830 AGATCT A^GATCT 3415 AGATCT BlnI 160 CCTAGG C^CTAGG 2421 CCTAGG BseRI 1234 CTCCTC GAGGAG(10/8) 2934 CTCCTC BsoBI 176 CCCGGG C^[CT]CG[GA]G 4448 CCCGGG BstYI 1830 AGATCT [GA]^GATC[CT] 3415 AGATCT DraIII 560 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG 3327 CACATGGTG DsaI 2694 CCATGG C^C[GA][CT]GG 4646 CCATGG EaeI 3562 TGGCCA [CT]^GGCC[GA] 4350 TGGCCA EcoRI 294 GAATTC G^AATTC 1359 GAATTC EcoT22I 1823 ATGCAT ATGCA^T 2190 ATGCAT HincII 1640 GTTGAC GT[CT]^[GA]AC 2865 GTCAAC HindII 1640 GTTGAC GT[CT]^[GA]AC 2865 GTCAAC HindIII 2587 AAGCTT A^AGCTT 4147 AAGCTT MflI 1830 AGATCT [GA]^GATC[CT] 3415 AGATCT MluNI 3562 TGGCCA TGG^CCA 4350 TGGCCA MscI 3562 TGGCCA TGG^CCA 4350 TGGCCA NcoI 2694 CCATGG C^CATGG 4646 CCATGG NsiI 1823 ATGCAT ATGCA^T 2190 ATGCAT NspI 942 ACATGT [GA]CATG^[CT] 3197 GCATGT PpuMI 1276 AGGTCCT [GA]G^G[AT]CC[CT] 4953 AGGACCC PstI 3074 CTGCAG CTGCA^G 4902 CTGCAG SmaI 176 CCCGGG CCC^GGG 4448 CCCGGG TaqI 1363 TCGA T^CGA 1748 TCGA TthHB8I 1363 TCGA T^CGA 1748 TCGA XhoII 1830 AGATCT [GA]^GATC[CT] 3415 AGATCT XmaCI 176 CCCGGG C^CCGGG 4448 CCCGGG XmaI 176 CCCGGG C^CCGGG 4448 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AseI AsnI Asp700I AspHI AvaII BbsI BbvI BciVI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PshBI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XmnI