##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
AocI Aor51HI AscI Asp718I AspI
AviII BamHI BbeI BbrPI BbvCI
BcgI BsaBI BsaHI BsiEI BsiWI
BspDI BspMI BsrBI BsrFI BssHII
BstBI BstEII BstPI Bsu36I Cfr10I
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
FseI FspI Hin1I HpaI KasI
KpnI KspI MamI MfeI MluI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SpeI SphI Sse8387I SwaI
Tth111I Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 860 TCCGGA T^CCGGA
AflII 417 CTTAAG C^TTAAG
AflIII 942 ACATGT A^C[GA][CT]GT
AhdI 238 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3776 GTGCAC G^TGCAC
ApaI 179 GGGCCC GGGCC^C
ApaLI 3776 GTGCAC G^TGCAC
AspEI 238 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 179 GGGCCC G[GA]GC[CT]^C
BclI 1504 TGATCA T^GATCA
BfrI 417 CTTAAG C^TTAAG
BglI 4392 GCCACTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 4616 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4616 GCTCAGC GC^T[ACGT]AGC
BsaAI 1240 TACGTA [CT]AC^GT[GA]
BsaWI 860 TCCGGA [AT]^CCGG[AT]
BseAI 860 TCCGGA T^CCGGA
BsgI 817 CTGCAC GTGCAG(16/14)
BsmBI 750 CGTCTC CGTCTC(1/5)
BspEI 860 TCCGGA T^CCGGA
BspHI 2288 TCATGA T^CATGA
BspLU11I 942 ACATGT A^CATGT
BssSI 4815 CACGAG CACGAG(-5/-1)
Bst1107I 1771 GTATAC GTA^TAC
BstAPI 3067 GCATAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 3689 CCAGCAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1771 GTATAC GTA^TAC
CelII 4616 GCTCAGC GC^T[ACGT]AGC
Eam1105I 238 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4227 CCTGGTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1504 TGATCA T^GATCA
HaeII 4850 AGCGCC [GA]GCGC^[CT]
HgaI 5165 GACGC GACGC(5/10)
MroI 860 TCCGGA T^CCGGA
RcaI 2288 TCATGA T^CATGA
SapI 4846 GAAGAGC GCTCTTC(1/4)
SnaBI 1240 TACGTA TAC^GTA
StuI 2394 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 831 GTCTAC GT^[AC][GT]AC
1771 GTATAC
AlwNI 493 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
3734 CAGAAACTG
AvaI 176 CCCGGG C^[CT]CG[GA]G
4448 CCCGGG
AvrII 160 CCTAGG C^CTAGG
2421 CCTAGG
BalI 3562 TGGCCA TGG^CCA
4350 TGGCCA
BanI 3426 GGTGCC G^G[CT][GA]CC
4892 GGCACC
BglII 1830 AGATCT A^GATCT
3415 AGATCT
BlnI 160 CCTAGG C^CTAGG
2421 CCTAGG
BseRI 1234 CTCCTC GAGGAG(10/8)
2934 CTCCTC
BsoBI 176 CCCGGG C^[CT]CG[GA]G
4448 CCCGGG
BstYI 1830 AGATCT [GA]^GATC[CT]
3415 AGATCT
DraIII 560 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
3327 CACATGGTG
DsaI 2694 CCATGG C^C[GA][CT]GG
4646 CCATGG
EaeI 3562 TGGCCA [CT]^GGCC[GA]
4350 TGGCCA
EcoRI 294 GAATTC G^AATTC
1359 GAATTC
EcoT22I 1823 ATGCAT ATGCA^T
2190 ATGCAT
HincII 1640 GTTGAC GT[CT]^[GA]AC
2865 GTCAAC
HindII 1640 GTTGAC GT[CT]^[GA]AC
2865 GTCAAC
HindIII 2587 AAGCTT A^AGCTT
4147 AAGCTT
MflI 1830 AGATCT [GA]^GATC[CT]
3415 AGATCT
MluNI 3562 TGGCCA TGG^CCA
4350 TGGCCA
MscI 3562 TGGCCA TGG^CCA
4350 TGGCCA
NcoI 2694 CCATGG C^CATGG
4646 CCATGG
NsiI 1823 ATGCAT ATGCA^T
2190 ATGCAT
NspI 942 ACATGT [GA]CATG^[CT]
3197 GCATGT
PpuMI 1276 AGGTCCT [GA]G^G[AT]CC[CT]
4953 AGGACCC
PstI 3074 CTGCAG CTGCA^G
4902 CTGCAG
SmaI 176 CCCGGG CCC^GGG
4448 CCCGGG
TaqI 1363 TCGA T^CGA
1748 TCGA
TthHB8I 1363 TCGA T^CGA
1748 TCGA
XhoII 1830 AGATCT [GA]^GATC[CT]
3415 AGATCT
XmaCI 176 CCCGGG C^CCGGG
4448 CCCGGG
XmaI 176 CCCGGG C^CCGGG
4448 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AvaII BbsI BbvI BciVI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PshBI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XmnI