##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI Aor51HI AscI Asp718I
AviII AvrII BamHI BbeI BbrPI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BspEI BsrFI BssHII BssSI BstBI
BstEII BstPI BstUI CelII Cfr10I
CpoI DraIII EagI EclXI Eco47III
Eco52I EcoO65I FauI FseI FspI
HaeII Hin1I KasI KpnI KspI
MamI MluI MroI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SwaI
Van91I
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AhdI 5043 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 2723 ATTAAT AT^TAAT
AsnI 2723 ATTAAT AT^TAAT
AspEI 5043 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2502 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 2595 GCAATGATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4731 GTATCC GTATCC(6/5)
BglI 4952 GCCCGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 4002 ACCGGA [AT]^CCGG[AT]
BspDI 4751 ATCGAT AT^CGAT
CfoI 1755 GCGC GCG^C
ClaI 4751 ATCGAT AT^CGAT
Eam1105I 5043 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 5264 GATATC GAT^ATC
HhaI 1755 GCGC GCG^C
HinP1I 1755 GCGC G^CGC
HpaI 3293 GTTAAC GTT^AAC
MaeII 2090 ACGT A^CGT
PflFI 2502 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2723 ATTAAT AT^TAAT
SalI 2781 GTCGAC G^TCGAC
SbfI 1201 CCTGCAGG CCTGCA^GG
SmaI 4953 CCCGGG CCC^GGG
SphI 4302 GCATGC GCATG^C
Sse8387I 1201 CCTGCAGG CCTGCA^GG
Tth111I 2502 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 4953 CCCGGG C^CCGGG
XmaI 4953 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 3474 GTGCAC G^TGCAC
4383 GTGCAC
AocI 3811 CCTGAGG CC^T[ACGT]AGG
4481 CCTGAGG
ApaI 2854 GGGCCC GGGCC^C
5375 GGGCCC
ApaLI 3474 GTGCAC G^TGCAC
4383 GTGCAC
BalI 3222 TGGCCA TGG^CCA
3270 TGGCCA
BspHI 3195 TCATGA T^CATGA
5277 TCATGA
BsrBI 3354 CCGCTC CCGCTC(-3/-3)
5056 CCGCTC
Bst1107I 1872 GTATAC GTA^TAC
6247 GTATAC
BstZ17I 1872 GTATAC GTA^TAC
6247 GTATAC
Bsu36I 3811 CCTGAGG CC^T[ACGT]AGG
4481 CCTGAGG
DraI 3862 TTTAAA TTT^AAA
6221 TTTAAA
DrdI 1052 GACACCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3652 GACTCAAATGTC
Eco81I 3811 CCTGAGG CC^T[ACGT]AGG
4481 CCTGAGG
EcoNI 1201 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4762 CCTGAGACAGG
HincII 2781 GTCGAC GT[CT]^[GA]AC
3293 GTTAAC
HindII 2781 GTCGAC GT[CT]^[GA]AC
3293 GTTAAC
MfeI 254 CAATTG C^AATTG
1212 CAATTG
MluNI 3222 TGGCCA TGG^CCA
3270 TGGCCA
MscI 3222 TGGCCA TGG^CCA
3270 TGGCCA
MunI 254 CAATTG C^AATTG
1212 CAATTG
NcoI 2198 CCATGG C^CATGG
2850 CCATGG
PaeR7I 585 CTCGAG C^TCGAG
4307 CTCGAG
RcaI 3195 TCATGA T^CATGA
5277 TCATGA
ScaI 973 AGTACT AGT^ACT
4972 AGTACT
XbaI 4522 TCTAGA T^CTAGA
6352 TCTAGA
XhoI 585 CTCGAG C^TCGAG
4307 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BclI
BcnI BfaI BfrI BglII BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspLU11I BspMI BsrDI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRI EcoRII EcoT14I EcoT22I FbaI
Fnu4HI FokI HaeIII HapII HgaI
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NheI NlaIII NlaIV NsiI NspI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI