##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AclI AcsI AflII AflIII ApoI
AscI AseI AsnI AvrII BbrPI
BfrI BlnI BsaAI BsiWI BspDI
BspLU11I BssHII Bst1107I BstBI BstZ17I
ClaI DraI DrdI EcoRI EcoRV
EcoT22I FseI HpaI KspI MfeI
MluI MunI NdeI NotI NruI
NsiI NspV PacI PmaCI PmeI
PmlI PshBI Psp1406I PvuI SacII
SalI SfiI SfuI SnaBI SpeI
SphI SspI SwaI TfiI Tsp509I
XbaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 758 GACGTC GACGT^C
Acc65I 595 GGTACC G^GTACC
AccIII 95 TCCGGA T^CCGGA
AhdI 1335 GACCGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1609 GTGCAC G^TGCAC
ApaLI 1609 GTGCAC G^TGCAC
Asp718I 595 GGTACC G^GTACC
AspEI 1335 GACCGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 616 GGATAC GTATCC(6/5)
BglII 2314 AGATCT A^GATCT
BsaBI 1540 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 95 TCCGGA T^CCGGA
BsiEI 3389 CGGCCG CG[GA][CT]^CG
BsmBI 2802 GAGACG CGTCTC(1/5)
BspEI 95 TCCGGA T^CCGGA
BsrDI 2012 GCAATG GCAATG(2/0)
BssSI 209 CTCGTG CACGAG(-5/-1)
BstAPI 1647 GCATTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 97 CGGACCG CG^G[AT]CCG
DraIII 338 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3389 CGGCCG C^GGCCG
Eam1105I 1335 GACCGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3389 CGGCCG C^GGCCG
Eco52I 3389 CGGCCG C^GGCCG
HincII 3871 GTTGAC GT[CT]^[GA]AC
HindII 3871 GTTGAC GT[CT]^[GA]AC
HindIII 2774 AAGCTT A^AGCTT
KpnI 595 GGTACC GGTAC^C
MamI 1540 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 95 TCCGGA T^CCGGA
MseI 2580 TTAA T^TAA
NheI 66 GCTAGC G^CTAGC
NspI 2793 GCATGT [GA]CATG^[CT]
RsrII 97 CGGACCG CG^G[AT]CCG
SbfI 3475 CCTGCAGG CCTGCA^GG
ScaI 2781 AGTACT AGT^ACT
SgrAI 1277 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 3475 CCTGCAGG CCTGCA^GG
Tru9I 2580 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1909 GTCTAC GT^[AC][GT]AC
3829 GTCTAC
AgeI 227 ACCGGT A^CCGGT
841 ACCGGT
AocI 3706 CCTGAGG CC^T[ACGT]AGG
3813 CCTGAGG
Asp700I 2674 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3640 GAAGCGCTTC
AviII 122 TGCGCA TGC^GCA
1668 TGCGCA
BamHI 363 GGATCC G^GATCC
2584 GGATCC
BbeI 2807 GGCGCC GGCGC^C
3376 GGCGCC
BcgI 648 CGAAGCGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
201 GCAGCCCTCTCG
BclI 831 TGATCA T^GATCA
2490 TGATCA
BsmI 1218 GCATTC GAATGC(1/-1)
1775 GCATTC
Bsp1407I 996 TGTACA T^GTACA
2988 TGTACA
BsrGI 996 TGTACA T^GTACA
2988 TGTACA
Bsu36I 3706 CCTGAGG CC^T[ACGT]AGG
3813 CCTGAGG
Eco81I 3706 CCTGAGG CC^T[ACGT]AGG
3813 CCTGAGG
FbaI 831 TGATCA T^GATCA
2490 TGATCA
FspI 122 TGCGCA TGC^GCA
1668 TGCGCA
KasI 2807 GGCGCC G^GCGCC
3376 GGCGCC
NarI 2807 GGCGCC GG^CGCC
3376 GGCGCC
PaeR7I 2537 CTCGAG C^TCGAG
2893 CTCGAG
PinAI 227 ACCGGT A^CCGGT
841 ACCGGT
PshAI 1960 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3874 GACTACTGTC
SfoI 2807 GGCGCC GGC^GCC
3376 GGCGCC
SmlI 2537 CTCGAG C^T[CT][GA]AG
2893 CTCGAG
SspBI 996 TGTACA T^GTACA
2988 TGTACA
StuI 903 AGGCCT AGG^CCT
2121 AGGCCT
XhoI 2537 CTCGAG C^TCGAG
2893 CTCGAG
XmnI 2674 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3640 GAAGCGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco47III
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PflFI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
SacI SapI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmaI StyI
TaqI TseI Tsp45I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI