##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
Acc65I AccIII AclI AscI Asp718I
AspI BalI BcgI BglII BsaBI
BseAI BsiWI BsmI BspDI BspEI
BspHI BstEII BstPI ClaI CpoI
EcoO65I EcoRI HpaI KpnI MamI
MluI MluNI MroI MscI NotI
NruI PacI PflFI PshAI Psp1406I
PvuI RcaI RsrII SalI SfiI
SnaBI SpeI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 3111 GACGTC GACGT^C
AhdI 817 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4173 GTGCAC G^TGCAC
ApaLI 4173 GTGCAC G^TGCAC
AseI 6491 ATTAAT AT^TAAT
AsnI 6491 ATTAAT AT^TAAT
Asp700I 1878 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 817 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4620 TGCGCA TGC^GCA
BamHI 6134 GGATCC G^GATCC
BciVI 5713 GGATAC GTATCC(6/5)
BclI 3262 TGATCA T^GATCA
BglI 1419 GCCCTACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 2835 GGTCTC GGTCTC(1/5)
BsiEI 10 CGGCCG CG[GA][CT]^CG
Bsp1407I 3610 TGTACA T^GTACA
BspLU11I 4844 ACATGT A^CATGT
BsrGI 3610 TGTACA T^GTACA
Bst1107I 3625 GTATAC GTA^TAC
BstBI 1867 TTCGAA TT^CGAA
BstXI 2520 CCAGCACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3625 GTATAC GTA^TAC
EagI 10 CGGCCG C^GGCCG
Eam1105I 817 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 10 CGGCCG C^GGCCG
Eco52I 10 CGGCCG C^GGCCG
EcoRV 2930 GATATC GAT^ATC
EcoT22I 3059 ATGCAT ATGCA^T
FbaI 3262 TGATCA T^GATCA
FseI 877 GGCCGGCC GGCCGG^CC
FspI 4620 TGCGCA TGC^GCA
KspI 2126 CCGCGG CCGC^GG
NcoI 702 CCATGG C^CATGG
NdeI 3284 CATATG CA^TATG
NsiI 3059 ATGCAT ATGCA^T
NspV 1867 TTCGAA TT^CGAA
PmeI 4287 GTTTAAAC GTTT^AAAC
PpuMI 344 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 6491 ATTAAT AT^TAAT
SacII 2126 CCGCGG CCGC^GG
ScaI 5293 AGTACT AGT^ACT
SexAI 458 ACCAGGT A^CC[AT]GGT
SfuI 1867 TTCGAA TT^CGAA
SgrAI 6417 CACCGGTG C[GA]^CCGG[CT]G
SphI 2249 GCATGC GCATG^C
SspBI 3610 TGTACA T^GTACA
XmnI 1878 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AgeI 3300 ACCGGT A^CCGGT
6418 ACCGGT
AocI 150 CCTCAGG CC^T[ACGT]AGG
6324 CCTGAGG
Aor51HI 581 AGCGCT AGC^GCT
1915 AGCGCT
AvrII 2911 CCTAGG C^CTAGG
3497 CCTAGG
BbrPI 5632 CACGTG CAC^GTG
6102 CACGTG
BbvCI 2769 CCTCAGC CCTCAGC(-5/-2)
4540 GCTGAGG
BlnI 2911 CCTAGG C^CTAGG
3497 CCTAGG
BsaAI 5632 CACGTG [CT]AC^GT[GA]
6102 CACGTG
BsmBI 2034 CGTCTC CGTCTC(1/5)
1075 GAGACG
BsrDI 4119 CATTGC GCAATG(2/0)
2899 GCAATG
BssHII 188 GCGCGC G^CGCGC
329 GCGCGC
Bsu36I 150 CCTCAGG CC^T[ACGT]AGG
6324 CCTGAGG
DraIII 676 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
2797 CACAGCGTG
DrdI 935 GACCAAACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2461 GACACGTCCGTC
Eco47III 581 AGCGCT AGC^GCT
1915 AGCGCT
Eco81I 150 CCTCAGG CC^T[ACGT]AGG
6324 CCTGAGG
MfeI 3121 CAATTG C^AATTG
5516 CAATTG
MunI 3121 CAATTG C^AATTG
5516 CAATTG
NheI 2184 GCTAGC G^CTAGC
5185 GCTAGC
PaeR7I 1321 CTCGAG C^TCGAG
4795 CTCGAG
PinAI 3300 ACCGGT A^CCGGT
6418 ACCGGT
PmaCI 5632 CACGTG CAC^GTG
6102 CACGTG
PmlI 5632 CACGTG CAC^GTG
6102 CACGTG
SapI 2319 GAAGAGC GCTCTTC(1/4)
2701 GAAGAGC
SbfI 108 CCTGCAGG CCTGCA^GG
621 CCTGCAGG
Sse8387I 108 CCTGCAGG CCTGCA^GG
621 CCTGCAGG
XcmI 254 CCAACCGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2304 CCACAGCACCAATGG
XhoI 1321 CTCGAG C^TCGAG
4795 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AflIII AluI AlwI
AlwNI ApaI ApoI AspHI AvaI
AvaII BanI BanII BbeI BbsI
BbvI BcnI BfaI BfrI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmaCI XmaI