################################## # NO CUT ENZYMES ( 45 enzymes) # ################################## Acc65I AccIII AclI AscI Asp718I AspI BalI BcgI BglII BsaBI BseAI BsiWI BsmI BspDI BspEI BspHI BstEII BstPI ClaI CpoI EcoO65I EcoRI HpaI KpnI MamI MluI MluNI MroI MscI NotI NruI PacI PflFI PshAI Psp1406I PvuI RcaI RsrII SalI SfiI SnaBI SpeI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 3111 GACGTC GACGT^C AhdI 817 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 4173 GTGCAC G^TGCAC ApaLI 4173 GTGCAC G^TGCAC AseI 6491 ATTAAT AT^TAAT AsnI 6491 ATTAAT AT^TAAT Asp700I 1878 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 817 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 4620 TGCGCA TGC^GCA BamHI 6134 GGATCC G^GATCC BciVI 5713 GGATAC GTATCC(6/5) BclI 3262 TGATCA T^GATCA BglI 1419 GCCCTACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 2835 GGTCTC GGTCTC(1/5) BsiEI 10 CGGCCG CG[GA][CT]^CG Bsp1407I 3610 TGTACA T^GTACA BspLU11I 4844 ACATGT A^CATGT BsrGI 3610 TGTACA T^GTACA Bst1107I 3625 GTATAC GTA^TAC BstBI 1867 TTCGAA TT^CGAA BstXI 2520 CCAGCACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 3625 GTATAC GTA^TAC EagI 10 CGGCCG C^GGCCG Eam1105I 817 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 10 CGGCCG C^GGCCG Eco52I 10 CGGCCG C^GGCCG EcoRV 2930 GATATC GAT^ATC EcoT22I 3059 ATGCAT ATGCA^T FbaI 3262 TGATCA T^GATCA FseI 877 GGCCGGCC GGCCGG^CC FspI 4620 TGCGCA TGC^GCA KspI 2126 CCGCGG CCGC^GG NcoI 702 CCATGG C^CATGG NdeI 3284 CATATG CA^TATG NsiI 3059 ATGCAT ATGCA^T NspV 1867 TTCGAA TT^CGAA PmeI 4287 GTTTAAAC GTTT^AAAC PpuMI 344 AGGACCT [GA]G^G[AT]CC[CT] PshBI 6491 ATTAAT AT^TAAT SacII 2126 CCGCGG CCGC^GG ScaI 5293 AGTACT AGT^ACT SexAI 458 ACCAGGT A^CC[AT]GGT SfuI 1867 TTCGAA TT^CGAA SgrAI 6417 CACCGGTG C[GA]^CCGG[CT]G SphI 2249 GCATGC GCATG^C SspBI 3610 TGTACA T^GTACA XmnI 1878 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AgeI 3300 ACCGGT A^CCGGT 6418 ACCGGT AocI 150 CCTCAGG CC^T[ACGT]AGG 6324 CCTGAGG Aor51HI 581 AGCGCT AGC^GCT 1915 AGCGCT AvrII 2911 CCTAGG C^CTAGG 3497 CCTAGG BbrPI 5632 CACGTG CAC^GTG 6102 CACGTG BbvCI 2769 CCTCAGC CCTCAGC(-5/-2) 4540 GCTGAGG BlnI 2911 CCTAGG C^CTAGG 3497 CCTAGG BsaAI 5632 CACGTG [CT]AC^GT[GA] 6102 CACGTG BsmBI 2034 CGTCTC CGTCTC(1/5) 1075 GAGACG BsrDI 4119 CATTGC GCAATG(2/0) 2899 GCAATG BssHII 188 GCGCGC G^CGCGC 329 GCGCGC Bsu36I 150 CCTCAGG CC^T[ACGT]AGG 6324 CCTGAGG DraIII 676 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG 2797 CACAGCGTG DrdI 935 GACCAAACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2461 GACACGTCCGTC Eco47III 581 AGCGCT AGC^GCT 1915 AGCGCT Eco81I 150 CCTCAGG CC^T[ACGT]AGG 6324 CCTGAGG MfeI 3121 CAATTG C^AATTG 5516 CAATTG MunI 3121 CAATTG C^AATTG 5516 CAATTG NheI 2184 GCTAGC G^CTAGC 5185 GCTAGC PaeR7I 1321 CTCGAG C^TCGAG 4795 CTCGAG PinAI 3300 ACCGGT A^CCGGT 6418 ACCGGT PmaCI 5632 CACGTG CAC^GTG 6102 CACGTG PmlI 5632 CACGTG CAC^GTG 6102 CACGTG SapI 2319 GAAGAGC GCTCTTC(1/4) 2701 GAAGAGC SbfI 108 CCTGCAGG CCTGCA^GG 621 CCTGCAGG Sse8387I 108 CCTGCAGG CCTGCA^GG 621 CCTGCAGG XcmI 254 CCAACCGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2304 CCACAGCACCAATGG XhoI 1321 CTCGAG C^TCGAG 4795 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 138 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflII AflIII AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BanI BanII BbeI BbsI BbvI BcnI BfaI BfrI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI