##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
AccIII AclI AgeI AocI AseI
AsnI BbsI BpuAI BsaWI BseAI
BspEI Bst1107I BstZ17I Bsu36I CpoI
DraI EagI EclXI Eco52I Eco81I
EcoT22I FseI HpaI MfeI MluI
MroI MunI NotI NruI NsiI
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SbfI SgrAI
Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 6010 GACGTC GACGT^C
AflII 798 CTTAAG C^TTAAG
AhdI 3163 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 6812 GGGCCC GGGCC^C
AscI 60 GGCGCGCC GG^CGCGCC
Asp700I 3093 GAACCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3163 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4272 CCTAGG C^CTAGG
BbeI 4531 GGCGCC GGCGC^C
BciVI 841 GTATCC GTATCC(6/5)
BfrI 798 CTTAAG C^TTAAG
BlnI 4272 CCTAGG C^CTAGG
BsaBI 2952 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2451 CGACCG CG[GA][CT]^CG
BsiWI 3603 CGTACG C^GTACG
BspHI 6467 TCATGA T^CATGA
BsrDI 3564 CATTGC GCAATG(2/0)
BssHII 61 GCGCGC G^CGCGC
BssSI 1873 CTCGTG CACGAG(-5/-1)
BstBI 2744 TTCGAA TT^CGAA
Eam1105I 3163 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
KasI 4531 GGCGCC G^GCGCC
KspI 6318 CCGCGG CCGC^GG
MamI 2952 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 4531 GGCGCC GG^CGCC
NdeI 4788 CATATG CA^TATG
NspV 2744 TTCGAA TT^CGAA
RcaI 6467 TCATGA T^CATGA
SacII 6318 CCGCGG CCGC^GG
SalI 207 GTCGAC G^TCGAC
SapI 2232 GAAGAGC GCTCTTC(1/4)
ScaI 3805 AGTACT AGT^ACT
SexAI 4383 ACCAGGT A^CC[AT]GGT
SfoI 4531 GGCGCC GGC^GCC
SfuI 2744 TTCGAA TT^CGAA
SnaBI 1535 TACGTA TAC^GTA
SpeI 5434 ACTAGT A^CTAGT
SspI 1450 AATATT AAT^ATT
StuI 2276 AGGCCT AGG^CCT
XmnI 3093 GAACCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Aor51HI 3278 AGCGCT AGC^GCT
4775 AGCGCT
AspI 1486 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
6010 GACGTCGTC
AviII 2294 TGCGCA TGC^GCA
6281 TGCGCA
BamHI 674 GGATCC G^GATCC
6546 GGATCC
BcgI 715 CGATGCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4995 CGACTGCTTTGC
BglII 287 AGATCT A^GATCT
2933 AGATCT
Bsp1407I 6587 TGTACA T^GTACA
6852 TGTACA
BspDI 4237 ATCGAT AT^CGAT
5338 ATCGAT
BspLU11I 2906 ACATGT A^CATGT
6743 ACATGT
BsrGI 6587 TGTACA T^GTACA
6852 TGTACA
ClaI 4237 ATCGAT AT^CGAT
5338 ATCGAT
DraIII 2329 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
3641 CACTGGGTG
DrdI 166 GACGGGTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4660 GACAGCGTGGTC
Eco47III 3278 AGCGCT AGC^GCT
4775 AGCGCT
EcoRI 6337 GAATTC G^AATTC
6682 GAATTC
FspI 2294 TGCGCA TGC^GCA
6281 TGCGCA
NheI 2079 GCTAGC G^CTAGC
4104 GCTAGC
PaeR7I 2968 CTCGAG C^TCGAG
3166 CTCGAG
PflFI 1486 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
6010 GACGTCGTC
SfiI 2034 GGCCAGTAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
6303 GGCCTTCGTGGCC
SmaI 5361 CCCGGG CCC^GGG
6473 CCCGGG
SphI 3513 GCATGC GCATG^C
6284 GCATGC
SspBI 6587 TGTACA T^GTACA
6852 TGTACA
Tth111I 1486 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
6010 GACGTCGTC
XhoI 2968 CTCGAG C^TCGAG
3166 CTCGAG
XmaCI 5361 CCCGGG C^CCGGG
6473 CCCGGG
XmaI 5361 CCCGGG C^CCGGG
6473 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AcyI AfaI AflIII AluI Alw44I
AlwI AlwNI ApaLI ApoI Asp718I
AspHI AvaI AvaII BalI BanI
BanII BbrPI BbvCI BbvI BclI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoO65I EcoRII
EcoRV EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII