##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
AhdI Alw44I ApaLI ApoI AscI
AseI AsnI AspEI AvrII BbrPI
BciVI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsiWI BsmI BspDI
BspLU11I BstBI CelII ClaI DraI
DrdI Eam1105I EcoNI EcoRI EcoT22I
FseI HincII HindII HindIII HpaI
KspI MaeI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
SacII SalI ScaI SfuI SgrAI
SpeI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 837 TCCGGA T^CCGGA
AflIII 1491 ACGTGT A^C[GA][CT]GT
AviII 318 TGCGCA TGC^GCA
BamHI 549 GGATCC G^GATCC
BclI 2001 TGATCA T^GATCA
BsaBI 2278 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 837 TCCGGA T^CCGGA
Bsp1407I 3661 TGTACA T^GTACA
BspEI 837 TCCGGA T^CCGGA
BspHI 1998 TCATGA T^CATGA
BsrDI 2862 GCAATG GCAATG(2/0)
BsrGI 3661 TGTACA T^GTACA
Bst1107I 1297 GTATAC GTA^TAC
BstAPI 1561 GCAGCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1297 GTATAC GTA^TAC
CpoI 528 CGGACCG CG^G[AT]CCG
EcoRV 3049 GATATC GAT^ATC
FbaI 2001 TGATCA T^GATCA
FspI 318 TGCGCA TGC^GCA
MamI 2278 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 837 TCCGGA T^CCGGA
MseI 2214 TTAA T^TAA
PaeR7I 3725 CTCGAG C^TCGAG
RcaI 1998 TCATGA T^CATGA
RsrII 528 CGGACCG CG^G[AT]CCG
SapI 2629 GCTCTTC GCTCTTC(1/4)
SbfI 1507 CCTGCAGG CCTGCA^GG
SfiI 3642 GGCCATCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 2660 TACGTA TAC^GTA
SphI 1926 GCATGC GCATG^C
Sse8387I 1507 CCTGCAGG CCTGCA^GG
SspBI 3661 TGTACA T^GTACA
TfiI 680 GAATC G^A[AT]TC
Tru9I 2214 TTAA T^TAA
XhoI 3725 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 1289 GGTACC G^GTACC
3978 GGTACC
AccI 1010 GTCTAC GT^[AC][GT]AC
1297 GTATAC
AocI 1946 CCTGAGG CC^T[ACGT]AGG
3999 CCTCAGG
Asp718I 1289 GGTACC G^GTACC
3978 GGTACC
AspI 563 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3613 GACTGGGTC
BbsI 2069 GAAGAC GAAGAC(2/6)
2765 GAAGAC
BcgI 1243 CGAGGCCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2218 CGAGATCACTGC
BpuAI 2069 GAAGAC GAAGAC(2/6)
2765 GAAGAC
BsaAI 1294 CACGTA [CT]AC^GT[GA]
2660 TACGTA
BssHII 225 GCGCGC G^CGCGC
3871 GCGCGC
BstEII 217 GGTGACC G^GT[ACGT]ACC
3007 GGTGACC
BstPI 217 GGTGACC G^GT[ACGT]ACC
3007 GGTGACC
Bsu36I 1946 CCTGAGG CC^T[ACGT]AGG
3999 CCTCAGG
DraIII 2689 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
3526 CACTTGGTG
EarI 2630 CTCTTC CTCTTC(1/4)
3594 CTCTTC
Eco81I 1946 CCTGAGG CC^T[ACGT]AGG
3999 CCTCAGG
EcoO65I 217 GGTGACC G^GT[ACGT]ACC
3007 GGTGACC
KpnI 1289 GGTACC GGTAC^C
3978 GGTACC
Ksp632I 2630 CTCTTC CTCTTC(1/4)
3594 CTCTTC
NaeI 2783 GCCGGC GCC^GGC
3794 GCCGGC
NgoMIV 2783 GCCGGC G^CCGGC
3794 GCCGGC
PflFI 563 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3613 GACTGGGTC
PflMI 2688 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3028 CCAGTGGGTGG
SexAI 2520 ACCTGGT A^CC[AT]GGT
2690 ACCTGGT
SmlI 3083 CTCAAG C^T[CT][GA]AG
3725 CTCGAG
Tth111I 563 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3613 GACTGGGTC
Van91I 2688 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3028 CCAGTGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbeI BbvCI BbvI BcnI
BglI BmyI BpmI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EclXI
Eco47III Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI StuI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI XmnI