##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflIII AgeI AscI AviII BamHI
BbeI BbrPI BcgI BciVI BclI
BsaBI BsaHI BseAI BsiEI BsiWI
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstXI BstZ17I Cfr10I ClaI
CpoI DrdI EagI EclXI Eco52I
EcoNI EcoT22I FbaI FseI FspI
Hin1I KasI MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 490 GGTACC G^GTACC
AhdI 2358 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1853 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2489 CCTCAGG CC^T[ACGT]AGG
Aor51HI 739 AGCGCT AGC^GCT
Asp718I 490 GGTACC G^GTACC
AspEI 2358 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 668 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3352 CCTAGG C^CTAGG
BalI 3403 TGGCCA TGG^CCA
BanI 490 GGTACC G^G[CT][GA]CC
BglI 2465 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1377 AGATCT A^GATCT
BlnI 3352 CCTAGG C^CTAGG
BlpI 2502 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2502 GCTAAGC GC^T[ACGT]AGC
BsaAI 1731 CACGTA [CT]AC^GT[GA]
BsaWI 1830 ACCGGA [AT]^CCGG[AT]
BsmBI 393 CGTCTC CGTCTC(1/5)
Bsp1407I 1003 TGTACA T^GTACA
BspHI 185 TCATGA T^CATGA
BspMI 352 ACCTGC ACCTGC(4/8)
BsrGI 1003 TGTACA T^GTACA
Bsu36I 2489 CCTCAGG CC^T[ACGT]AGG
CelII 2502 GCTAAGC GC^T[ACGT]AGC
DraI 1275 TTTAAA TTT^AAA
DraIII 3396 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2358 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 739 AGCGCT AGC^GCT
Eco81I 2489 CCTCAGG CC^T[ACGT]AGG
HpaI 1175 GTTAAC GTT^AAC
KpnI 490 GGTACC GGTAC^C
MluNI 3403 TGGCCA TGG^CCA
MscI 3403 TGGCCA TGG^CCA
NspI 2322 ACATGC [GA]CATG^[CT]
PflFI 668 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 501 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1344 GACTGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 185 TCATGA T^CATGA
SapI 1709 GAAGAGC GCTCTTC(1/4)
SexAI 669 ACCTGGT A^CC[AT]GGT
SspBI 1003 TGTACA T^GTACA
Tth111I 668 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 501 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 616 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflII 1901 CTTAAG C^TTAAG
2928 CTTAAG
Alw44I 1812 GTGCAC G^TGCAC
1980 GTGCAC
ApaI 150 GGGCCC GGGCC^C
2781 GGGCCC
ApaLI 1812 GTGCAC G^TGCAC
1980 GTGCAC
AvaI 100 CTCGGG C^[CT]CG[GA]G
194 CTCGGG
BbsI 839 GTCTTC GAAGAC(2/6)
1281 GTCTTC
BfrI 1901 CTTAAG C^TTAAG
2928 CTTAAG
BpuAI 839 GTCTTC GAAGAC(2/6)
1281 GTCTTC
BsgI 690 CTGCAC GTGCAG(16/14)
1850 GTGCAG
BsoBI 100 CTCGGG C^[CT]CG[GA]G
194 CTCGGG
BstYI 1377 AGATCT [GA]^GATC[CT]
2223 GGATCT
EcoRV 1076 GATATC GAT^ATC
3455 GATATC
HgaI 157 GCGTC GACGC(5/10)
761 GCGTC
HincII 348 GTTGAC GT[CT]^[GA]AC
1175 GTTAAC
HindII 348 GTTGAC GT[CT]^[GA]AC
1175 GTTAAC
HindIII 1615 AAGCTT A^AGCTT
2505 AAGCTT
KspI 61 CCGCGG CCGC^GG
130 CCGCGG
MflI 1377 AGATCT [GA]^GATC[CT]
2223 GGATCT
SacII 61 CCGCGG CCGC^GG
130 CCGCGG
SspI 3122 AATATT AAT^ATT
3286 AATATT
XhoII 1377 AGATCT [GA]^GATC[CT]
2223 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AvaII BanII BbvCI BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoO65I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PshBI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmnI