##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AscI
Asp700I Asp718I AspEI AspI AviII
BamHI BbeI BbrPI BcgI BclI
BfrI BglI BglII BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DraIII EagI Eam1105I EarI EclXI
Eco52I EcoRI EcoRV FbaI FseI
FspI HindIII HpaI HphI KasI
KpnI Ksp632I MamI MfeI MluI
MroI MunI NaeI NarI NcoI
NgoMIV NheI NotI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SpeI
Sse8387I SspBI SspI SwaI TfiI
Tth111I Van91I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcyI 1052 GGCGTC G[GA]^CG[CT]C
Alw44I 1109 GTGCAC G^TGCAC
ApaLI 1109 GTGCAC G^TGCAC
AseI 1592 ATTAAT AT^TAAT
AsnI 1592 ATTAAT AT^TAAT
BbsI 303 GTCTTC GAAGAC(2/6)
BbvCI 1494 CCTCAGC CCTCAGC(-5/-2)
BciVI 1237 GGATAC GTATCC(6/5)
BpuAI 303 GTCTTC GAAGAC(2/6)
BsaAI 186 TACGTA [CT]AC^GT[GA]
BsaHI 1052 GGCGTC G[GA]^CG[CT]C
BsaI 417 GAGACC GGTCTC(1/5)
BsmI 1251 GCATTC GAATGC(1/-1)
BsrBI 847 CCGCTC CCGCTC(-3/-3)
BsrFI 571 ACCGGC [GA]^CCGG[CT]
BstAPI 158 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1655 GGTAACC G^GT[ACGT]ACC
BstPI 1655 GGTAACC G^GT[ACGT]ACC
BstXI 1216 CCAGGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 571 ACCGGC [GA]^CCGG[CT]
EcoO65I 1655 GGTAACC G^GT[ACGT]ACC
EcoT22I 1384 ATGCAT ATGCA^T
Hin1I 1052 GGCGTC G[GA]^CG[CT]C
HincII 1852 GTTGAC GT[CT]^[GA]AC
HindII 1852 GTTGAC GT[CT]^[GA]AC
HinfI 30 GACTC G^A[ACGT]TC
KspI 129 CCGCGG CCGC^GG
NdeI 1398 CATATG CA^TATG
NruI 33 TCGCGA TCG^CGA
NsiI 1384 ATGCAT ATGCA^T
PleI 30 GACTC GAGTC(4/5)
PshAI 1855 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1592 ATTAAT AT^TAAT
PvuII 1466 CAGCTG CAG^CTG
SacI 744 GAGCTC GAGCT^C
SacII 129 CCGCGG CCGC^GG
ScaI 1432 AGTACT AGT^ACT
SmlI 516 CTCAAG C^T[CT][GA]AG
SnaBI 186 TACGTA TAC^GTA
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcsI 1377 AAATTT [GA]^AATT[CT]
1513 AAATTC
AlwNI 159 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
372 CAGCTCCTG
AocI 836 CCTCAGG CC^T[ACGT]AGG
1868 CCTAAGG
Aor51HI 181 AGCGCT AGC^GCT
286 AGCGCT
ApaI 903 GGGCCC GGGCC^C
1119 GGGCCC
ApoI 1377 AAATTT [GA]^AATT[CT]
1513 AAATTC
AvrII 506 CCTAGG C^CTAGG
1488 CCTAGG
BanI 96 GGCACC G^G[CT][GA]CC
720 GGTGCC
BlnI 506 CCTAGG C^CTAGG
1488 CCTAGG
BlpI 1537 GCTCAGC GC^T[ACGT]AGC
1818 GCTGAGC
Bpu1102I 1537 GCTCAGC GC^T[ACGT]AGC
1818 GCTGAGC
BsmAI 417 GAGAC GTCTC(1/5)
468 GTCTC
BstYI 1082 GGATCT [GA]^GATC[CT]
1702 GGATCT
Bsu36I 836 CCTCAGG CC^T[ACGT]AGG
1868 CCTAAGG
CelII 1537 GCTCAGC GC^T[ACGT]AGC
1818 GCTGAGC
DrdI 144 GACCAGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
459 GACCACGCTGTC
DsaI 129 CCGCGG C^C[GA][CT]GG
685 CCACGG
Eco47III 181 AGCGCT AGC^GCT
286 AGCGCT
Eco81I 836 CCTCAGG CC^T[ACGT]AGG
1868 CCTAAGG
EcoNI 1321 CCTTCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1778 CCTCCAGCAGG
HgaI 346 GCGTC GACGC(5/10)
1053 GCGTC
MaeII 187 ACGT A^CGT
1622 ACGT
MboII 245 GAAGA GAAGA(8/7)
304 TCTTC
MflI 1082 GGATCT [GA]^GATC[CT]
1702 GGATCT
MslI 776 CACTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1140 CATTTCGGTG
PstI 156 CTGCAG CTGCA^G
703 CTGCAG
SmaI 112 CCCGGG CCC^GGG
883 CCCGGG
SphI 391 GCATGC GCATG^C
614 GCATGC
TaqI 436 TCGA T^CGA
814 TCGA
Tsp45I 589 GTGAC ^GT[GC]AC
933 GTCAC
TthHB8I 436 TCGA T^CGA
814 TCGA
XbaI 1415 TCTAGA T^CTAGA
1830 TCTAGA
XcmI 316 CCATGAACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1639 CCATCCCACAAGTGG
XhoII 1082 GGATCT [GA]^GATC[CT]
1702 GGATCT
XmaCI 112 CCCGGG C^CCGGG
883 CCCGGG
XmaI 112 CCCGGG C^CCGGG
883 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BalI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII ItaI MaeI
MaeIII MboI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
Tru9I TseI Tsp509I TspRI