##################################
# NO CUT ENZYMES ( 175 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseRI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstNI BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRII EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MflI MluI MseI
MvaI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccIII 43 TCCGGA T^CCGGA
AciI 160 CCGC CCGC(-3/-1)
AcsI 39 AAATTC [GA]^AATT[CT]
AfaI 228 GTAC GT^AC
AlwI 133 GGATC GGATC(4/5)
ApoI 39 AAATTC [GA]^AATT[CT]
AspHI 213 GAGCTC G[AT]GC[AT]^C
BalI 187 TGGCCA TGG^CCA
BanII 213 GAGCTC G[GA]GC[CT]^C
BbsI 58 GTCTTC GAAGAC(2/6)
BcnI 391 CCCGG CC^[GC]GG
BmyI 213 GAGCTC G[AGT]GC[ACT]^C
BpmI 428 CTCCAG CTGGAG(16/14)
BpuAI 58 GTCTTC GAAGAC(2/6)
BseAI 43 TCCGGA T^CCGGA
BsgI 376 GTGCAG GTGCAG(16/14)
BsiHKAI 213 GAGCTC G[AT]GC[AT]^C
BsmFI 140 GGGAC GGGAC(10/14)
Bsp1286I 213 GAGCTC G[AGT]GC[ACT]^C
BspEI 43 TCCGGA T^CCGGA
BsrDI 416 CATTGC GCAATG(2/0)
BssKI 391 CCCGG ^CC[ACGT]GG
BstAPI 399 GCATCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 408 GCTGGC GC[ACGT]^[ACGT]GC
DdeI 216 CTCAG C^T[ACGT]AG
DpnII 134 GATC ^GATC
DraII 24 GGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 187 TGGCCA [CT]^GGCC[GA]
EcoO109I 24 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 52 CCTTGG C^C[AT][AT]GG
FauI 159 CCCGC CCCGC(4/6)
MaeII 17 ACGT A^CGT
MaeIII 175 GTGAC ^GT[ACGT]AC
MboI 134 GATC ^GATC
MfeI 80 CAATTG C^AATTG
MluNI 187 TGGCCA TGG^CCA
MroI 43 TCCGGA T^CCGGA
MscI 187 TGGCCA TGG^CCA
MspA1I 160 CCGCTG C[AC]G^C[GT]G
MunI 80 CAATTG C^AATTG
MwoI 399 GCATCCAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 391 CCCGG CC^[GC]GG
NdeII 134 GATC ^GATC
PleI 310 GAGTC GAGTC(4/5)
PpuMI 24 GGGTCCT [GA]G^G[AT]CC[CT]
RsaI 228 GTAC GT^AC
SacI 213 GAGCTC GAGCT^C
Sau3AI 134 GATC ^GATC
ScrFI 391 CCCGG CC^[ACGT]GG
SfaNI 399 GCATC GCATC(5/9)
StyI 52 CCTTGG C^C[AT][AT]GG
TfiI 424 GATTC G^A[AT]TC
Tsp45I 175 GTGAC ^GT[GC]AC
TspRI 238 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AluI 71 AGCT AG^CT
214 AGCT
AvaII 25 GGTCC G^G[AT]CC
357 GGACC
BbvI 412 GCAGC GCAGC(8/12)
162 GCTGC
BsaJI 52 CCTTGG C^C[ACGT][ACGT]GG
390 CCCCGG
BsaWI 43 TCCGGA [AT]^CCGG[AT]
62 TCCGGT
BsiYI 124 CCGTGAAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
159 CCCGCTGCTGG
BslI 124 CCGTGAAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
159 CCCGCTGCTGG
BsrI 360 CCAGT ACTGG(1/-1)
430 CCAGT
BstF5I 231 CATCC GGATG(2/0)
400 CATCC
Fnu4HI 162 GCTGC GC^[ACGT]GC
412 GCAGC
FokI 231 CATCC GGATG(9/13)
400 CATCC
HaeIII 188 GGCC GG^CC
388 GGCC
HinfI 310 GAGTC G^A[ACGT]TC
424 GATTC
HphI 174 GGTGA GGTGA(8/7)
182 GGTGA
ItaI 162 GCTGC GC^[ACGT]GC
412 GCAGC
MboII 297 GAAGA GAAGA(8/7)
59 TCTTC
MnlI 28 CCTC CCTC(7/6)
13 GAGG
MslI 119 CATCTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
179 CATGGTGATG
NlaIII 179 CATG CATG^
421 CATG
TaqI 6 TCGA T^CGA
248 TCGA
TseI 162 GCTGC G^C[AT]GC
412 GCAGC
Tsp509I 40 AATT ^AATT
81 AATT
TthHB8I 6 TCGA T^CGA
248 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
#########################################
Cfr13I HapII HpaII MspI NlaIV
Sau96I