##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AviII BamHI BcgI BciVI BglI
BlpI Bpu1102I BseAI BsiWI BspEI
BspMI BsrDI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CelII CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV FseI FspI HpaI
KpnI KspI MluI MroI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NspV PacI PflFI PmeI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StuI Tth111I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AcyI 208 GGCGCC G[GA]^CG[CT]C
AflII 2763 CTTAAG C^TTAAG
AflIII 3695 ACATGT A^C[GA][CT]GT
AgeI 708 ACCGGT A^CCGGT
AseI 3674 ATTAAT AT^TAAT
AsnI 3674 ATTAAT AT^TAAT
Asp700I 356 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 2141 CCTAGG C^CTAGG
BanI 208 GGCGCC G^G[CT][GA]CC
BbeI 208 GGCGCC GGCGC^C
BbrPI 396 CACGTG CAC^GTG
BfrI 2763 CTTAAG C^TTAAG
BglII 1727 AGATCT A^GATCT
BlnI 2141 CCTAGG C^CTAGG
BsaAI 396 CACGTG [CT]AC^GT[GA]
BsaHI 208 GGCGCC G[GA]^CG[CT]C
BsmBI 1659 GAGACG CGTCTC(1/5)
Bsp1407I 3707 TGTACA T^GTACA
BspDI 765 ATCGAT AT^CGAT
BspHI 3101 TCATGA T^CATGA
BspLU11I 3695 ACATGT A^CATGT
BsrBI 3683 CCGCTC CCGCTC(-3/-3)
BsrFI 708 ACCGGT [GA]^CCGG[CT]
BsrGI 3707 TGTACA T^GTACA
BstXI 125 CCAGTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1727 AGATCT [GA]^GATC[CT]
CfoI 209 GCGC GCG^C
Cfr10I 708 ACCGGT [GA]^CCGG[CT]
ClaI 765 ATCGAT AT^CGAT
EcoT22I 3245 ATGCAT ATGCA^T
HaeII 208 GGCGCC [GA]GCGC^[CT]
HgaI 1180 GCGTC GACGC(5/10)
HhaI 209 GCGC GCG^C
Hin1I 208 GGCGCC G[GA]^CG[CT]C
HinP1I 209 GCGC G^CGC
HincII 1115 GTTGAC GT[CT]^[GA]AC
HindII 1115 GTTGAC GT[CT]^[GA]AC
KasI 208 GGCGCC G^GCGCC
MfeI 216 CAATTG C^AATTG
MflI 1727 AGATCT [GA]^GATC[CT]
MunI 216 CAATTG C^AATTG
NarI 208 GGCGCC GG^CGCC
NcoI 2843 CCATGG C^CATGG
NsiI 3245 ATGCAT ATGCA^T
PaeR7I 1080 CTCGAG C^TCGAG
PflMI 3783 CCATCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 708 ACCGGT A^CCGGT
PmaCI 396 CACGTG CAC^GTG
PmlI 396 CACGTG CAC^GTG
PshBI 3674 ATTAAT AT^TAAT
RcaI 3101 TCATGA T^CATGA
SapI 849 GAAGAGC GCTCTTC(1/4)
ScaI 1034 AGTACT AGT^ACT
SfaNI 2929 GATGC GCATC(5/9)
SfoI 208 GGCGCC GGC^GCC
SphI 2006 GCATGC GCATG^C
SspBI 3707 TGTACA T^GTACA
SwaI 3438 ATTTAAAT ATTT^AAAT
Van91I 3783 CCATCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1080 CTCGAG C^TCGAG
XhoII 1727 AGATCT [GA]^GATC[CT]
XmnI 356 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AocI 1040 CCTAAGG CC^T[ACGT]AGG
1751 CCTGAGG
BalI 3109 TGGCCA TGG^CCA
3459 TGGCCA
BbvCI 24 CCTCAGC CCTCAGC(-5/-2)
80 GCTGAGG
BsaI 1674 GAGACC GGTCTC(1/5)
2147 GGTCTC
BsaWI 273 ACCGGA [AT]^CCGG[AT]
708 ACCGGT
BseRI 2369 CTCCTC GAGGAG(10/8)
891 GAGGAG
BsgI 1731 CTGCAC GTGCAG(16/14)
381 GTGCAG
BsmI 1459 GAATGC GAATGC(1/-1)
1615 GAATGC
Bsu36I 1040 CCTAAGG CC^T[ACGT]AGG
1751 CCTGAGG
Eco81I 1040 CCTAAGG CC^T[ACGT]AGG
1751 CCTGAGG
EcoNI 653 CCTAGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3330 CCTAGATTAGG
EcoT14I 2141 CCTAGG C^C[AT][AT]GG
2843 CCATGG
FauI 3682 CCCGC CCCGC(4/6)
897 GCGGG
HindIII 2525 AAGCTT A^AGCTT
3149 AAGCTT
MaeII 397 ACGT A^CGT
522 ACGT
MluNI 3109 TGGCCA TGG^CCA
3459 TGGCCA
MscI 3109 TGGCCA TGG^CCA
3459 TGGCCA
NspI 2006 GCATGC [GA]CATG^[CT]
3695 ACATGT
PstI 2014 CTGCAG CTGCA^G
3378 CTGCAG
SacI 946 GAGCTC GAGCT^C
1504 GAGCTC
SexAI 2194 ACCTGGT A^CC[AT]GGT
2555 ACCAGGT
StyI 2141 CCTAGG C^C[AT][AT]GG
2843 CCATGG
XbaI 1351 TCTAGA T^CTAGA
2961 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BanII BbsI BbvI BclI BcnI
BfaI BmyI BpmI BpuAI BsaBI
BsaJI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRI EcoRII
FbaI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MamI MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I