##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI Aor51HI Asp718I AspI AviII
AvrII BamHI BbsI BcgI BciVI
BfrI BglII BlnI BpuAI BsaBI
BsiWI BspDI BspHI BssSI Bst1107I
BstZ17I ClaI DraIII DrdI Eco47III
EcoRV EcoT22I FseI FspI KpnI
MamI MfeI MluI MunI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI Psp1406I
PvuI RcaI SalI SbfI ScaI
SfiI SgrAI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccIII 355 TCCGGA T^CCGGA
AhdI 1149 GACAGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4041 GTGCAC G^TGCAC
AlwNI 816 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2811 GGGCCC GGGCC^C
ApaLI 4041 GTGCAC G^TGCAC
AscI 450 GGCGCGCC GG^CGCGCC
Asp700I 2375 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1149 GACAGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1327 TGGCCA TGG^CCA
BbeI 280 GGCGCC GGCGC^C
BbrPI 120 CACGTG CAC^GTG
BbvCI 324 CCTCAGC CCTCAGC(-5/-2)
BsaI 1976 GGTCTC GGTCTC(1/5)
BseAI 355 TCCGGA T^CCGGA
BspEI 355 TCCGGA T^CCGGA
BsrDI 3815 GCAATG GCAATG(2/0)
BstAPI 126 GCATCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1178 TTCGAA TT^CGAA
BstEII 2597 GGTTACC G^GT[ACGT]ACC
BstPI 2597 GGTTACC G^GT[ACGT]ACC
BstXI 4176 CCAGGCATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 251 CGGACCG CG^G[AT]CCG
DraII 2811 GGGCCCC [GA]G^G[ACGT]CC[CT]
EagI 335 CGGCCG C^GGCCG
Eam1105I 1149 GACAGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 335 CGGCCG C^GGCCG
Eco52I 335 CGGCCG C^GGCCG
EcoNI 2816 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2811 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 2597 GGTTACC G^GT[ACGT]ACC
EcoRI 2923 GAATTC G^AATTC
HindIII 3213 AAGCTT A^AGCTT
HpaI 1293 GTTAAC GTT^AAC
KasI 280 GGCGCC G^GCGCC
KspI 338 CCGCGG CCGC^GG
MluNI 1327 TGGCCA TGG^CCA
MroI 355 TCCGGA T^CCGGA
MscI 1327 TGGCCA TGG^CCA
NarI 280 GGCGCC GG^CGCC
NdeI 4181 CATATG CA^TATG
NheI 857 GCTAGC G^CTAGC
NspV 1178 TTCGAA TT^CGAA
PflMI 1875 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 120 CACGTG CAC^GTG
PmlI 120 CACGTG CAC^GTG
RsrII 251 CGGACCG CG^G[AT]CCG
SacII 338 CCGCGG CCGC^GG
SexAI 3382 ACCAGGT A^CC[AT]GGT
SfoI 280 GGCGCC GGC^GCC
SfuI 1178 TTCGAA TT^CGAA
StuI 289 AGGCCT AGG^CCT
Van91I 1875 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1963 CCACCCCAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2375 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 280 GGCGCC G[GA]^CG[CT]C
1307 GACGCC
AseI 2916 ATTAAT AT^TAAT
4219 ATTAAT
AsnI 2916 ATTAAT AT^TAAT
4219 ATTAAT
BclI 2480 TGATCA T^GATCA
3735 TGATCA
BlpI 1268 GCTCAGC GC^T[ACGT]AGC
3939 GCTGAGC
Bpu1102I 1268 GCTCAGC GC^T[ACGT]AGC
3939 GCTGAGC
BsaAI 120 CACGTG [CT]AC^GT[GA]
799 TACGTG
BsaHI 280 GGCGCC G[GA]^CG[CT]C
1307 GACGCC
BsaWI 346 ACCGGA [AT]^CCGG[AT]
355 TCCGGA
BsmBI 216 CGTCTC CGTCTC(1/5)
3071 CGTCTC
BspMI 435 ACCTGC ACCTGC(4/8)
1865 ACCTGC
BssHII 451 GCGCGC G^CGCGC
2303 GCGCGC
CelII 1268 GCTCAGC GC^T[ACGT]AGC
3939 GCTGAGC
FbaI 2480 TGATCA T^GATCA
3735 TGATCA
Hin1I 280 GGCGCC G[GA]^CG[CT]C
1307 GACGCC
HincII 1293 GTTAAC GT[CT]^[GA]AC
3836 GTTGAC
HindII 1293 GTTAAC GT[CT]^[GA]AC
3836 GTTGAC
PshBI 2916 ATTAAT AT^TAAT
4219 ATTAAT
SmaI 826 CCCGGG CCC^GGG
2233 CCCGGG
SmlI 1011 CTTGAG C^T[CT][GA]AG
2424 CTTGAG
XbaI 1410 TCTAGA T^CTAGA
2966 TCTAGA
XmaCI 826 CCCGGG C^CCGGG
2233 CCCGGG
XmaI 826 CCCGGG C^CCGGG
2233 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AocI ApoI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspLU11I BsrBI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DsaI EaeI EarI Eco81I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PleI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII