##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BcgI
BciVI BlnI BlpI Bpu1102I BsaBI
BsaHI BsiWI BsmBI Bsp1407I BspDI
BspLU11I BsrBI BsrFI BsrGI BssHII
Bst1107I BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV FseI FspI Hin1I
KasI KpnI MamI MluI NaeI
NarI NdeI NgoMIV NheI NotI
NruI PacI PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI Sse8387I SspBI
StuI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 3995 GTAGAC GT^[AC][GT]AC
AccII 15 CGCG CG^CG
AccIII 934 TCCGGA T^CCGGA
AflII 2077 CTTAAG C^TTAAG
AflIII 4030 ACGTGT A^C[GA][CT]GT
ApaI 562 GGGCCC GGGCC^C
BanI 114 GGCACC G^G[CT][GA]CC
BbvCI 3578 CCTCAGC CCTCAGC(-5/-2)
BcnI 3465 CCGGG CC^[GC]GG
BfrI 2077 CTTAAG C^TTAAG
BglI 3472 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3566 AGATCT A^GATCT
BsaI 3460 GGTCTC GGTCTC(1/5)
BsaWI 934 TCCGGA [AT]^CCGG[AT]
BseAI 934 TCCGGA T^CCGGA
BsiEI 17 CGGTCG CG[GA][CT]^CG
BspEI 934 TCCGGA T^CCGGA
BssSI 839 CACGAG CACGAG(-5/-1)
BstEII 1151 GGTCACC G^GT[ACGT]ACC
BstPI 1151 GGTCACC G^GT[ACGT]ACC
BstUI 15 CGCG CG^CG
BstXI 3381 CCAATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoNI 3865 CCTAGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1151 GGTCACC G^GT[ACGT]ACC
FauI 1053 CCCGC CCCGC(4/6)
HgaI 2580 GCGTC GACGC(5/10)
HindIII 926 AAGCTT A^AGCTT
HpaI 352 GTTAAC GTT^AAC
KspI 14 CCGCGG CCGC^GG
MroI 934 TCCGGA T^CCGGA
MvnI 15 CGCG CG^CG
NciI 3465 CCGGG CC^[GC]GG
PshAI 3333 GACTTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 3349 CTGCAG CTGCA^G
SacI 2492 GAGCTC GAGCT^C
SacII 14 CCGCGG CCGC^GG
SapI 1543 GAAGAGC GCTCTTC(1/4)
ScaI 1731 AGTACT AGT^ACT
SpeI 1183 ACTAGT A^CTAGT
SphI 4016 GCATGC GCATG^C
SwaI 656 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 3581 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
3983 CAGTTCCTG
AseI 1708 ATTAAT AT^TAAT
3049 ATTAAT
AsnI 1708 ATTAAT AT^TAAT
3049 ATTAAT
BalI 2595 TGGCCA TGG^CCA
2606 TGGCCA
BanII 562 GGGCCC G[GA]GC[CT]^C
2492 GAGCTC
BbsI 142 GAAGAC GAAGAC(2/6)
1882 GAAGAC
BclI 771 TGATCA T^GATCA
2895 TGATCA
BpuAI 142 GAAGAC GAAGAC(2/6)
1882 GAAGAC
BsgI 2484 GTGCAG GTGCAG(16/14)
3788 GTGCAG
BsmFI 572 GGGAC GGGAC(10/14)
2709 GGGAC
BspHI 1281 TCATGA T^CATGA
3435 TCATGA
DraI 657 TTTAAA TTT^AAA
4220 TTTAAA
FbaI 771 TGATCA T^GATCA
2895 TGATCA
HaeII 10 AGCGCC [GA]GCGC^[CT]
3760 AGCGCC
HapII 935 CCGG C^CGG
3465 CCGG
HpaII 935 CCGG C^CGG
3465 CCGG
MluNI 2595 TGGCCA TGG^CCA
2606 TGGCCA
MscI 2595 TGGCCA TGG^CCA
2606 TGGCCA
MspI 935 CCGG C^CGG
3465 CCGG
NcoI 479 CCATGG C^CATGG
3473 CCATGG
PaeR7I 2228 CTCGAG C^TCGAG
3308 CTCGAG
PpuMI 486 AGGACCT [GA]G^G[AT]CC[CT]
765 GGGTCCT
PshBI 1708 ATTAAT AT^TAAT
3049 ATTAAT
RcaI 1281 TCATGA T^CATGA
3435 TCATGA
XhoI 2228 CTCGAG C^TCGAG
3308 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaI AvaII
BbvI BfaI BmyI BpmI BsaAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstBI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HhaI
HinP1I HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MunI MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI NspV PflMI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfuI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XcmI XhoII XmnI