##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AlwNI AscI AviII AvrII BbrPI
BcgI BfrI BglI BlnI BpmI
BsaBI BsaI BsiWI BspDI Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
DrdI EcoRI EcoRV FseI FspI
HincII HindII HpaI KspI MamI
MfeI MluI MunI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI Psp1406I RsrII SacII
SalI ScaI SfiI SfuI SgrAI
SmaI SpeI SwaI XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 845 GGTACC G^GTACC
AhdI 1709 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 775 CCTGAGG CC^T[ACGT]AGG
ApaI 1549 GGGCCC GGGCC^C
AseI 3697 ATTAAT AT^TAAT
AsnI 3697 ATTAAT AT^TAAT
Asp718I 845 GGTACC G^GTACC
AspEI 1709 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3205 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 2184 CCTCAGC CCTCAGC(-5/-2)
BciVI 2692 GTATCC GTATCC(6/5)
BglII 1519 AGATCT A^GATCT
BlpI 3345 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3345 GCTGAGC GC^T[ACGT]AGC
BsaAI 3037 TACGTA [CT]AC^GT[GA]
BsmBI 698 GAGACG CGTCTC(1/5)
BsmI 3316 GAATGC GAATGC(1/-1)
BspHI 2521 TCATGA T^CATGA
BssHII 97 GCGCGC G^CGCGC
BstXI 3560 CCATCCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 775 CCTGAGG CC^T[ACGT]AGG
CelII 3345 GCTGAGC GC^T[ACGT]AGC
EagI 156 CGGCCG C^GGCCG
Eam1105I 1709 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 156 CGGCCG C^GGCCG
Eco52I 156 CGGCCG C^GGCCG
Eco81I 775 CCTGAGG CC^T[ACGT]AGG
EcoT22I 595 ATGCAT ATGCA^T
HindIII 395 AAGCTT A^AGCTT
KpnI 845 GGTACC GGTAC^C
NdeI 2403 CATATG CA^TATG
NheI 300 GCTAGC G^CTAGC
NsiI 595 ATGCAT ATGCA^T
PaeR7I 1753 CTCGAG C^TCGAG
PflFI 3205 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3697 ATTAAT AT^TAAT
PvuI 2172 CGATCG CGAT^CG
RcaI 2521 TCATGA T^CATGA
SacI 2954 GAGCTC GAGCT^C
SbfI 1921 CCTGCAGG CCTGCA^GG
SexAI 1276 ACCAGGT A^CC[AT]GGT
SnaBI 3037 TACGTA TAC^GTA
Sse8387I 1921 CCTGCAGG CCTGCA^GG
StuI 2092 AGGCCT AGG^CCT
Tth111I 3205 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1753 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 32 TCCGGA T^CCGGA
169 TCCGGA
AflIII 2472 ACATGT A^C[GA][CT]GT
3355 ACATGT
Aor51HI 52 AGCGCT AGC^GCT
2363 AGCGCT
Asp700I 656 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2314 GAACATCTTC
BalI 456 TGGCCA TGG^CCA
3128 TGGCCA
BbeI 641 GGCGCC GGCGC^C
2301 GGCGCC
BbsI 971 GAAGAC GAAGAC(2/6)
2417 GTCTTC
BclI 3084 TGATCA T^GATCA
3171 TGATCA
BpuAI 971 GAAGAC GAAGAC(2/6)
2417 GTCTTC
BsaWI 32 TCCGGA [AT]^CCGG[AT]
169 TCCGGA
BseAI 32 TCCGGA T^CCGGA
169 TCCGGA
BspEI 32 TCCGGA T^CCGGA
169 TCCGGA
BspLU11I 2472 ACATGT A^CATGT
3355 ACATGT
BspMI 1613 ACCTGC ACCTGC(4/8)
1924 GCAGGT
BssSI 1212 CACGAG CACGAG(-5/-1)
2481 CTCGTG
BstEII 110 GGTTACC G^GT[ACGT]ACC
577 GGTGACC
BstPI 110 GGTTACC G^GT[ACGT]ACC
577 GGTGACC
DraI 407 TTTAAA TTT^AAA
1265 TTTAAA
DraIII 1487 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
3227 CACGCCGTG
Eco47III 52 AGCGCT AGC^GCT
2363 AGCGCT
EcoNI 771 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1231 CCTCAGTAAGG
EcoO65I 110 GGTTACC G^GT[ACGT]ACC
577 GGTGACC
FbaI 3084 TGATCA T^GATCA
3171 TGATCA
KasI 641 GGCGCC G^GCGCC
2301 GGCGCC
MluNI 456 TGGCCA TGG^CCA
3128 TGGCCA
MroI 32 TCCGGA T^CCGGA
169 TCCGGA
MscI 456 TGGCCA TGG^CCA
3128 TGGCCA
NaeI 26 GCCGGC GCC^GGC
154 GCCGGC
NarI 641 GGCGCC GG^CGCC
2301 GGCGCC
NgoMIV 26 GCCGGC G^CCGGC
154 GCCGGC
PflMI 2122 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3226 CCACGCCGTGG
PvuII 758 CAGCTG CAG^CTG
2426 CAGCTG
SfaNI 1165 GATGC GCATC(5/9)
3500 GATGC
SfoI 641 GGCGCC GGC^GCC
2301 GGCGCC
SphI 593 GCATGC GCATG^C
3219 GCATGC
Van91I 2122 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3226 CCACGCCGTGG
XcmI 450 CCAAAATGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1983 CCATCGGTCCCCTGG
XmnI 656 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2314 GAACATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI ApaLI ApoI
AspHI AvaI AvaII BamHI BanI
BanII BbvI BcnI BfaI BmyI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BsrBI BsrDI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI RsaI
SapI Sau3AI Sau96I ScrFI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII