##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AvrII BamHI
BcgI BciVI BfrI BglII BlnI
BsaBI BsgI BsiEI BsiWI Bsp1407I
BspDI BspHI BspLU11I BsrGI BssHII
Bst1107I BstAPI BstYI BstZ17I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoT22I FseI HgaI KpnI MamI
MfeI MflI MluI MunI NcoI
NdeI NotI NruI NsiI PacI
PaeR7I PflMI PinAI PleI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfiI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Van91I XbaI XcmI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccIII 199 TCCGGA T^CCGGA
AcyI 132 GGCGCC G[GA]^CG[CT]C
AocI 1338 CCTCAGG CC^T[ACGT]AGG
Asp700I 965 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 600 GAGCTC G[AT]GC[AT]^C
AspI 38 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 807 TGCGCA TGC^GCA
BalI 1281 TGGCCA TGG^CCA
BanI 132 GGCGCC G^G[CT][GA]CC
BbeI 132 GGCGCC GGCGC^C
BbrPI 788 CACGTG CAC^GTG
BbsI 374 GAAGAC GAAGAC(2/6)
BpuAI 374 GAAGAC GAAGAC(2/6)
BsaAI 788 CACGTG [CT]AC^GT[GA]
BsaHI 132 GGCGCC G[GA]^CG[CT]C
BsaI 2116 GGTCTC GGTCTC(1/5)
BseAI 199 TCCGGA T^CCGGA
BsiHKAI 600 GAGCTC G[AT]GC[AT]^C
BsmBI 353 GAGACG CGTCTC(1/5)
BsmI 680 GCATTC GAATGC(1/-1)
BspEI 199 TCCGGA T^CCGGA
BssSI 708 CTCGTG CACGAG(-5/-1)
BstBI 162 TTCGAA TT^CGAA
BstEII 43 GGTCACC G^GT[ACGT]ACC
BstPI 43 GGTCACC G^GT[ACGT]ACC
Bsu36I 1338 CCTCAGG CC^T[ACGT]AGG
Eco81I 1338 CCTCAGG CC^T[ACGT]AGG
EcoO65I 43 GGTCACC G^GT[ACGT]ACC
FauI 156 GCGGG CCCGC(4/6)
FspI 807 TGCGCA TGC^GCA
Hin1I 132 GGCGCC G[GA]^CG[CT]C
HincII 1433 GTTAAC GT[CT]^[GA]AC
HindII 1433 GTTAAC GT[CT]^[GA]AC
HindIII 1845 AAGCTT A^AGCTT
HpaI 1433 GTTAAC GTT^AAC
KasI 132 GGCGCC G^GCGCC
MluNI 1281 TGGCCA TGG^CCA
MroI 199 TCCGGA T^CCGGA
MscI 1281 TGGCCA TGG^CCA
NaeI 250 GCCGGC GCC^GGC
NarI 132 GGCGCC GG^CGCC
NgoMIV 250 GCCGGC G^CCGGC
NspV 162 TTCGAA TT^CGAA
PflFI 38 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 788 CACGTG CAC^GTG
PmlI 788 CACGTG CAC^GTG
SacI 600 GAGCTC GAGCT^C
SfoI 132 GGCGCC GGC^GCC
SfuI 162 TTCGAA TT^CGAA
SmaI 1092 CCCGGG CCC^GGG
SphI 1988 GCATGC GCATG^C
TaqI 163 TCGA T^CGA
Tth111I 38 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 163 TCGA T^CGA
XmaCI 1092 CCCGGG C^CCGGG
XmaI 1092 CCCGGG C^CCGGG
XmnI 965 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 70 AAATTC [GA]^AATT[CT]
1463 AAATTC
ApoI 70 AAATTC [GA]^AATT[CT]
1463 AAATTC
BbvCI 206 CCTCAGC CCTCAGC(-5/-2)
23 GCTGAGG
BclI 660 TGATCA T^GATCA
1936 TGATCA
BlpI 141 GCTCAGC GC^T[ACGT]AGC
1505 GCTAAGC
Bpu1102I 141 GCTCAGC GC^T[ACGT]AGC
1505 GCTAAGC
BseRI 339 GAGGAG GAGGAG(10/8)
889 GAGGAG
BspMI 530 ACCTGC ACCTGC(4/8)
1856 GCAGGT
BsrBI 195 CCGCTC CCGCTC(-3/-3)
622 CCGCTC
BsrDI 664 CATTGC GCAATG(2/0)
2039 GCAATG
BsrFI 152 ACCGGC [GA]^CCGG[CT]
250 GCCGGC
BstXI 669 CCAACACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1026 CCACCTGGCTGG
CelII 141 GCTCAGC GC^T[ACGT]AGC
1505 GCTAAGC
Cfr10I 152 ACCGGC [GA]^CCGG[CT]
250 GCCGGC
DraI 67 TTTAAA TTT^AAA
1459 TTTAAA
DsaI 98 CCGCGG C^C[GA][CT]GG
136 CCGCGG
EaeI 1227 TGGCCG [CT]^GGCC[GA]
1281 TGGCCA
EarI 1055 CTCTTC CTCTTC(1/4)
1540 CTCTTC
EcoRV 272 GATATC GAT^ATC
1062 GATATC
EcoT14I 447 CCAAGG C^C[AT][AT]GG
1266 CCAAGG
FbaI 660 TGATCA T^GATCA
1936 TGATCA
HinfI 523 GATTC G^A[ACGT]TC
1762 GAATC
Ksp632I 1055 CTCTTC CTCTTC(1/4)
1540 CTCTTC
KspI 98 CCGCGG CCGC^GG
136 CCGCGG
MaeII 769 ACGT A^CGT
789 ACGT
MaeIII 44 GTCAC ^GT[ACGT]AC
711 GTGAC
MslI 475 CATCCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
937 CACGCTTGTG
NheI 103 GCTAGC G^CTAGC
120 GCTAGC
PstI 263 CTGCAG CTGCA^G
329 CTGCAG
SacII 98 CCGCGG CCGC^GG
136 CCGCGG
SmlI 1711 CTTGAG C^T[CT][GA]AG
1742 CTTGAG
StyI 447 CCAAGG C^C[AT][AT]GG
1266 CCAAGG
TfiI 523 GATTC G^A[AT]TC
1762 GAATC
Tsp45I 44 GTCAC ^GT[GC]AC
711 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI AvaII BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaJI
BsaWI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeI MboI MboII MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI Tru9I TseI
Tsp509I TspRI