##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
Acc65I AclI AocI ApaI AscI
Asp718I AvrII BbeI BbrPI BciVI
BlnI BsaBI BsiWI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CpoI Eco81I EcoO65I EcoRV FseI
HpaI KasI KpnI MamI NaeI
NarI NgoMIV NotI NruI NspV
PacI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SalI SbfI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 3198 GACGTC GACGT^C
AcyI 3198 GACGTC G[GA]^CG[CT]C
AflII 2579 CTTAAG C^TTAAG
AgeI 5058 ACCGGT A^CCGGT
AseI 3337 ATTAAT AT^TAAT
AsnI 3337 ATTAAT AT^TAAT
AviII 3391 TGCGCA TGC^GCA
BamHI 437 GGATCC G^GATCC
BbvCI 5875 CCTCAGC CCTCAGC(-5/-2)
BclI 6587 TGATCA T^GATCA
BfrI 2579 CTTAAG C^TTAAG
BglI 168 GCCTCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 7008 TACGTG [CT]AC^GT[GA]
BsaHI 3198 GACGTC G[GA]^CG[CT]C
BsiEI 6852 CGGCCG CG[GA][CT]^CG
BsmBI 30 CGTCTC CGTCTC(1/5)
Bsp1407I 2898 TGTACA T^GTACA
BspDI 3635 ATCGAT AT^CGAT
BspLU11I 4642 ACATGT A^CATGT
BsrFI 5058 ACCGGT [GA]^CCGG[CT]
BsrGI 2898 TGTACA T^GTACA
Cfr10I 5058 ACCGGT [GA]^CCGG[CT]
ClaI 3635 ATCGAT AT^CGAT
EagI 6852 CGGCCG C^GGCCG
EclXI 6852 CGGCCG C^GGCCG
Eco52I 6852 CGGCCG C^GGCCG
EcoRI 5789 GAATTC G^AATTC
FbaI 6587 TGATCA T^GATCA
FspI 3391 TGCGCA TGC^GCA
Hin1I 3198 GACGTC G[GA]^CG[CT]C
KspI 5100 CCGCGG CCGC^GG
NdeI 712 CATATG CA^TATG
NheI 6897 GCTAGC G^CTAGC
PaeR7I 58 CTCGAG C^TCGAG
PinAI 5058 ACCGGT A^CCGGT
PshBI 3337 ATTAAT AT^TAAT
SacI 3428 GAGCTC GAGCT^C
SacII 5100 CCGCGG CCGC^GG
SexAI 1749 ACCTGGT A^CC[AT]GGT
SfiI 167 GGCCTCCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 5451 CCCGGG CCC^GGG
SspBI 2898 TGTACA T^GTACA
XhoI 58 CTCGAG C^TCGAG
XmaCI 5451 CCCGGG C^CCGGG
XmaI 5451 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccIII 88 TCCGGA T^CCGGA
1074 TCCGGA
Aor51HI 836 AGCGCT AGC^GCT
6499 AGCGCT
AspI 1819 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
6096 GACCGTGTC
BglII 2438 AGATCT A^GATCT
4403 AGATCT
BlpI 2283 GCTTAGC GC^T[ACGT]AGC
5905 GCTGAGC
Bpu1102I 2283 GCTTAGC GC^T[ACGT]AGC
5905 GCTGAGC
BsaI 336 GAGACC GGTCTC(1/5)
5606 GAGACC
BseAI 88 TCCGGA T^CCGGA
1074 TCCGGA
BspEI 88 TCCGGA T^CCGGA
1074 TCCGGA
BspHI 1960 TCATGA T^CATGA
2253 TCATGA
BspMI 2539 ACCTGC ACCTGC(4/8)
5958 ACCTGC
BsrBI 5007 CCGCTC CCGCTC(-3/-3)
5744 GAGCGG
CelII 2283 GCTTAGC GC^T[ACGT]AGC
5905 GCTGAGC
DrdI 4750 GACTTATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7408 GACATTTGCGTC
Eco47III 836 AGCGCT AGC^GCT
6499 AGCGCT
HindIII 2463 AAGCTT A^AGCTT
5989 AAGCTT
MluI 15 ACGCGT A^CGCGT
219 ACGCGT
MroI 88 TCCGGA T^CCGGA
1074 TCCGGA
PflFI 1819 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
6096 GACCGTGTC
PvuII 383 CAGCTG CAG^CTG
5903 CAGCTG
RcaI 1960 TCATGA T^CATGA
2253 TCATGA
Tth111I 1819 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
6096 GACCGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AhdI AluI Alw44I AlwI
AlwNI ApaLI ApoI Asp700I AspEI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcgI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrDI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI Eam1105I EarI EcoNI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MunI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PpuMI PstI RsaI SapI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SphI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI