##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
Acc65I AclI AflII AgeI Alw44I
Aor51HI ApaLI AscI Asp718I AviII
BfrI BsaBI BsiWI BsrBI BssHII
BstBI CpoI Eco47III EcoT22I FseI
FspI KpnI KspI MamI MfeI
MluI MunI NaeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmeI Psp1406I PvuI RsrII
SacII SalI SfiI SfuI SgrAI
SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 1343 GTATAC GT^[AC][GT]AC
AccIII 661 TCCGGA T^CCGGA
AhdI 5224 GACCTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3432 GGGCCC GGGCC^C
AspEI 5224 GACCTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 6593 CCTAGG C^CTAGG
BbrPI 2810 CACGTG CAC^GTG
BcgI 1148 GCAACAAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 503 GGATAC GTATCC(6/5)
BglI 411 GCCCCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 6593 CCTAGG C^CTAGG
BseAI 661 TCCGGA T^CCGGA
BsiEI 1992 CGGCCG CG[GA][CT]^CG
BspDI 2298 ATCGAT AT^CGAT
BspEI 661 TCCGGA T^CCGGA
BspHI 3878 TCATGA T^CATGA
Bst1107I 1343 GTATAC GTA^TAC
BstZ17I 1343 GTATAC GTA^TAC
ClaI 2298 ATCGAT AT^CGAT
DraIII 5588 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1992 CGGCCG C^GGCCG
Eam1105I 5224 GACCTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1992 CGGCCG C^GGCCG
Eco52I 1992 CGGCCG C^GGCCG
EcoRI 739 GAATTC G^AATTC
EcoRV 2409 GATATC GAT^ATC
HpaI 6112 GTTAAC GTT^AAC
MroI 661 TCCGGA T^CCGGA
NdeI 6688 CATATG CA^TATG
PaeR7I 509 CTCGAG C^TCGAG
PmaCI 2810 CACGTG CAC^GTG
PmlI 2810 CACGTG CAC^GTG
RcaI 3878 TCATGA T^CATGA
SbfI 3029 CCTGCAGG CCTGCA^GG
SmaI 1597 CCCGGG CCC^GGG
SphI 6335 GCATGC GCATG^C
Sse8387I 3029 CCTGCAGG CCTGCA^GG
StuI 418 AGGCCT AGG^CCT
XhoI 509 CTCGAG C^TCGAG
XmaCI 1597 CCCGGG C^CCGGG
XmaI 1597 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 2092 CGCG CG^CG
2094 CGCG
AocI 414 CCTCAGG CC^T[ACGT]AGG
3049 CCTCAGG
AseI 3676 ATTAAT AT^TAAT
6243 ATTAAT
AsnI 3676 ATTAAT AT^TAAT
6243 ATTAAT
AspI 3513 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3516 GACGTCGTC
BamHI 1645 GGATCC G^GATCC
5031 GGATCC
BbsI 1493 GAAGAC GAAGAC(2/6)
3303 GTCTTC
BbvCI 57 CCTCAGC CCTCAGC(-5/-2)
1668 CCTCAGC
BclI 3478 TGATCA T^GATCA
3584 TGATCA
BglII 1589 AGATCT A^GATCT
6005 AGATCT
BpuAI 1493 GAAGAC GAAGAC(2/6)
3303 GTCTTC
BsaWI 661 TCCGGA [AT]^CCGG[AT]
2845 ACCGGA
Bsp1407I 289 TGTACA T^GTACA
2756 TGTACA
BspLU11I 146 ACATGT A^CATGT
4186 ACATGT
BsrGI 289 TGTACA T^GTACA
2756 TGTACA
BssSI 2130 CACGAG CACGAG(-5/-1)
4153 CTCGTG
BstUI 2092 CGCG CG^CG
2094 CGCG
Bsu36I 414 CCTCAGG CC^T[ACGT]AGG
3049 CCTCAGG
DrdI 3510 GACGACGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3513 GACGACGTCGTC
Eco81I 414 CCTCAGG CC^T[ACGT]AGG
3049 CCTCAGG
FbaI 3478 TGATCA T^GATCA
3584 TGATCA
MvnI 2092 CGCG CG^CG
2094 CGCG
PflFI 3513 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3516 GACGTCGTC
PshAI 1595 GACCCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5307 GACAGTGGTC
PshBI 3676 ATTAAT AT^TAAT
6243 ATTAAT
SapI 930 GCTCTTC GCTCTTC(1/4)
2189 GCTCTTC
SexAI 3145 ACCAGGT A^CC[AT]GGT
4312 ACCTGGT
SspBI 289 TGTACA T^GTACA
2756 TGTACA
SspI 4436 AATATT AAT^ATT
6196 AATATT
Tth111I 3513 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3516 GACGTCGTC
XbaI 4321 TCTAGA T^CTAGA
4834 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AatII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbeI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SfoI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI