##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccII AclI AcyI AflII
AgeI AhdI Alw44I ApaI ApaLI
AscI AspEI AspI AviII BbeI
BbrPI BbvCI BclI BfrI BsaAI
BsaBI BsaHI BsiEI BsiWI Bsp1407I
BspDI BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstBI BstUI BstZ17I
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco52I EcoRV EcoT22I FbaI
FseI FspI Hin1I HindIII HpaI
KasI KspI MamI MluI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1154 GGTACC G^GTACC
AccI 758 GTAGAC GT^[AC][GT]AC
AccIII 2427 TCCGGA T^CCGGA
AflIII 3625 ACATGT A^C[GA][CT]GT
Aor51HI 1636 AGCGCT AGC^GCT
AseI 824 ATTAAT AT^TAAT
AsnI 824 ATTAAT AT^TAAT
Asp700I 1772 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1154 GGTACC G^GTACC
AvaI 2265 CTCGAG C^[CT]CG[GA]G
BalI 3684 TGGCCA TGG^CCA
BanI 1154 GGTACC G^G[CT][GA]CC
BcgI 3156 GCATGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 149 CCCGG CC^[GC]GG
BglII 4147 AGATCT A^GATCT
BsaWI 2427 TCCGGA [AT]^CCGG[AT]
BseAI 2427 TCCGGA T^CCGGA
BsgI 3337 GTGCAG GTGCAG(16/14)
BsmI 3190 GCATTC GAATGC(1/-1)
BsoBI 2265 CTCGAG C^[CT]CG[GA]G
BspEI 2427 TCCGGA T^CCGGA
BspHI 42 TCATGA T^CATGA
BspLU11I 3625 ACATGT A^CATGT
BstXI 2616 CCACCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 1653 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 3385 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1552 GACGAGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 3684 TGGCCA [CT]^GGCC[GA]
Eco47III 1636 AGCGCT AGC^GCT
EcoO109I 1653 AGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 1981 GCGGG CCCGC(4/6)
HincII 919 GTTGAC GT[CT]^[GA]AC
HindII 919 GTTGAC GT[CT]^[GA]AC
KpnI 1154 GGTACC GGTAC^C
MfeI 1454 CAATTG C^AATTG
MluNI 3684 TGGCCA TGG^CCA
MroI 2427 TCCGGA T^CCGGA
MscI 3684 TGGCCA TGG^CCA
MunI 1454 CAATTG C^AATTG
NciI 149 CCCGG CC^[GC]GG
NcoI 1147 CCATGG C^CATGG
PaeR7I 2265 CTCGAG C^TCGAG
PshBI 824 ATTAAT AT^TAAT
RcaI 42 TCATGA T^CATGA
SacI 1349 GAGCTC GAGCT^C
XbaI 1000 TCTAGA T^CTAGA
XcmI 2591 CCAAAACAACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2265 CTCGAG C^TCGAG
XmnI 1772 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AvrII 389 CCTAGG C^CTAGG
4126 CCTAGG
BamHI 538 GGATCC G^GATCC
3472 GGATCC
BanII 1349 GAGCTC G[GA]GC[CT]^C
3678 GGGCTC
BciVI 598 GGATAC GTATCC(6/5)
3067 GGATAC
BglI 644 GCCTCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3402 GCCAGCAAGGC
BlnI 389 CCTAGG C^CTAGG
4126 CCTAGG
BlpI 2795 GCTGAGC GC^T[ACGT]AGC
2920 GCTGAGC
Bpu1102I 2795 GCTGAGC GC^T[ACGT]AGC
2920 GCTGAGC
BsaI 2076 GAGACC GGTCTC(1/5)
2665 GGTCTC
BssSI 507 CTCGTG CACGAG(-5/-1)
3126 CTCGTG
BstEII 3484 GGTGACC G^GT[ACGT]ACC
3882 GGTAACC
BstPI 3484 GGTGACC G^GT[ACGT]ACC
3882 GGTAACC
CelII 2795 GCTGAGC GC^T[ACGT]AGC
2920 GCTGAGC
EcoO65I 3484 GGTGACC G^GT[ACGT]ACC
3882 GGTAACC
EcoRI 63 GAATTC G^AATTC
2525 GAATTC
HapII 150 CCGG C^CGG
2428 CCGG
HpaII 150 CCGG C^CGG
2428 CCGG
MaeII 1843 ACGT A^CGT
2146 ACGT
MspI 150 CCGG C^CGG
2428 CCGG
SapI 1346 GAAGAGC GCTCTTC(1/4)
406 GCTCTTC
ScaI 2309 AGTACT AGT^ACT
4220 AGTACT
SphI 1360 GCATGC GCATG^C
1585 GCATGC
StuI 838 AGGCCT AGG^CCT
4136 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DsaI
EarI Eco81I EcoNI EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HgaI
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII