##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Aor51HI AscI Asp718I AspEI AspI
BbrPI BciVI BclI BfrI BglII
BsaBI BsiEI BsiWI BsmI BspHI
Bst1107I BstBI BstZ17I CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV FbaI FseI HpaI KpnI
MamI MfeI MluI MunI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RcaI RsrII SalI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 2213 GACGTC GACGT^C
AccI 3830 GTAGAC GT^[AC][GT]AC
AseI 1529 ATTAAT AT^TAAT
AsnI 1529 ATTAAT AT^TAAT
AviII 3204 TGCGCA TGC^GCA
BbeI 3219 GGCGCC GGCGC^C
BcgI 4400 GCAGGGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 3551 TACGTG [CT]AC^GT[GA]
Bsp1407I 2659 TGTACA T^GTACA
BspDI 3399 ATCGAT AT^CGAT
BsrBI 3467 GAGCGG CCGCTC(-3/-3)
BsrDI 2624 CATTGC GCAATG(2/0)
BsrFI 5042 GCCGGC [GA]^CCGG[CT]
BsrGI 2659 TGTACA T^GTACA
BssHII 3272 GCGCGC G^CGCGC
BssSI 3407 CACGAG CACGAG(-5/-1)
BstXI 3987 CCACCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 5042 GCCGGC [GA]^CCGG[CT]
ClaI 3399 ATCGAT AT^CGAT
DraI 1589 TTTAAA TTT^AAA
EcoRI 2741 GAATTC G^AATTC
FspI 3204 TGCGCA TGC^GCA
HincII 5225 GTCAAC GT[CT]^[GA]AC
HindII 5225 GTCAAC GT[CT]^[GA]AC
HindIII 3958 AAGCTT A^AGCTT
KasI 3219 GGCGCC G^GCGCC
KspI 3429 CCGCGG CCGC^GG
NaeI 5042 GCCGGC GCC^GGC
NarI 3219 GGCGCC GG^CGCC
NdeI 5373 CATATG CA^TATG
NgoMIV 5042 GCCGGC G^CCGGC
NheI 2353 GCTAGC G^CTAGC
PshAI 3086 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1529 ATTAAT AT^TAAT
SacII 3429 CCGCGG CCGC^GG
SapI 2722 GAAGAGC GCTCTTC(1/4)
SbfI 666 CCTGCAGG CCTGCA^GG
SfoI 3219 GGCGCC GGC^GCC
SphI 1758 GCATGC GCATG^C
Sse8387I 666 CCTGCAGG CCTGCA^GG
SspBI 2659 TGTACA T^GTACA
SspI 3968 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 2672 ACATGT A^C[GA][CT]GT
5528 ACATGT
Alw44I 3143 GTGCAC G^TGCAC
5207 GTGCAC
ApaI 3367 GGGCCC GGGCC^C
4441 GGGCCC
ApaLI 3143 GTGCAC G^TGCAC
5207 GTGCAC
Asp700I 1898 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3031 GAAGATGTTC
BalI 3156 TGGCCA TGG^CCA
4023 TGGCCA
BlpI 3652 GCTCAGC GC^T[ACGT]AGC
4073 GCTCAGC
Bpu1102I 3652 GCTCAGC GC^T[ACGT]AGC
4073 GCTCAGC
BsmBI 2102 GAGACG CGTCTC(1/5)
2211 GAGACG
BspLU11I 2672 ACATGT A^CATGT
5528 ACATGT
BstEII 584 GGTCACC G^GT[ACGT]ACC
4275 GGTGACC
BstPI 584 GGTCACC G^GT[ACGT]ACC
4275 GGTGACC
CelII 3652 GCTCAGC GC^T[ACGT]AGC
4073 GCTCAGC
DraIII 125 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
1274 CACCGTGTG
EcoO65I 584 GGTCACC G^GT[ACGT]ACC
4275 GGTGACC
EcoT22I 2981 ATGCAT ATGCA^T
5370 ATGCAT
HaeII 1111 GGCGCT [GA]GCGC^[CT]
3219 GGCGCC
MluNI 3156 TGGCCA TGG^CCA
4023 TGGCCA
MscI 3156 TGGCCA TGG^CCA
4023 TGGCCA
NsiI 2981 ATGCAT ATGCA^T
5370 ATGCAT
PaeR7I 2834 CTCGAG C^TCGAG
3673 CTCGAG
SmaI 458 CCCGGG CCC^GGG
772 CCCGGG
XhoI 2834 CTCGAG C^TCGAG
3673 CTCGAG
XmaCI 458 CCCGGG C^CCGGG
772 CCCGGG
XmaI 458 CCCGGG C^CCGGG
772 CCCGGG
XmnI 1898 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3031 GAAGATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII AvrII
BamHI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlnI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspEI BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MroI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII