################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## Acc65I AclI AflII AgeI AhdI Aor51HI AscI Asp718I AspEI AspI BbrPI BciVI BclI BfrI BglII BsaBI BsiEI BsiWI BsmI BspHI Bst1107I BstBI BstZ17I CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRV FbaI FseI HpaI KpnI MamI MfeI MluI MunI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SalI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 2213 GACGTC GACGT^C AccI 3830 GTAGAC GT^[AC][GT]AC AseI 1529 ATTAAT AT^TAAT AsnI 1529 ATTAAT AT^TAAT AviII 3204 TGCGCA TGC^GCA BbeI 3219 GGCGCC GGCGC^C BcgI 4400 GCAGGGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaAI 3551 TACGTG [CT]AC^GT[GA] Bsp1407I 2659 TGTACA T^GTACA BspDI 3399 ATCGAT AT^CGAT BsrBI 3467 GAGCGG CCGCTC(-3/-3) BsrDI 2624 CATTGC GCAATG(2/0) BsrFI 5042 GCCGGC [GA]^CCGG[CT] BsrGI 2659 TGTACA T^GTACA BssHII 3272 GCGCGC G^CGCGC BssSI 3407 CACGAG CACGAG(-5/-1) BstXI 3987 CCACCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 5042 GCCGGC [GA]^CCGG[CT] ClaI 3399 ATCGAT AT^CGAT DraI 1589 TTTAAA TTT^AAA EcoRI 2741 GAATTC G^AATTC FspI 3204 TGCGCA TGC^GCA HincII 5225 GTCAAC GT[CT]^[GA]AC HindII 5225 GTCAAC GT[CT]^[GA]AC HindIII 3958 AAGCTT A^AGCTT KasI 3219 GGCGCC G^GCGCC KspI 3429 CCGCGG CCGC^GG NaeI 5042 GCCGGC GCC^GGC NarI 3219 GGCGCC GG^CGCC NdeI 5373 CATATG CA^TATG NgoMIV 5042 GCCGGC G^CCGGC NheI 2353 GCTAGC G^CTAGC PshAI 3086 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1529 ATTAAT AT^TAAT SacII 3429 CCGCGG CCGC^GG SapI 2722 GAAGAGC GCTCTTC(1/4) SbfI 666 CCTGCAGG CCTGCA^GG SfoI 3219 GGCGCC GGC^GCC SphI 1758 GCATGC GCATG^C Sse8387I 666 CCTGCAGG CCTGCA^GG SspBI 2659 TGTACA T^GTACA SspI 3968 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflIII 2672 ACATGT A^C[GA][CT]GT 5528 ACATGT Alw44I 3143 GTGCAC G^TGCAC 5207 GTGCAC ApaI 3367 GGGCCC GGGCC^C 4441 GGGCCC ApaLI 3143 GTGCAC G^TGCAC 5207 GTGCAC Asp700I 1898 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3031 GAAGATGTTC BalI 3156 TGGCCA TGG^CCA 4023 TGGCCA BlpI 3652 GCTCAGC GC^T[ACGT]AGC 4073 GCTCAGC Bpu1102I 3652 GCTCAGC GC^T[ACGT]AGC 4073 GCTCAGC BsmBI 2102 GAGACG CGTCTC(1/5) 2211 GAGACG BspLU11I 2672 ACATGT A^CATGT 5528 ACATGT BstEII 584 GGTCACC G^GT[ACGT]ACC 4275 GGTGACC BstPI 584 GGTCACC G^GT[ACGT]ACC 4275 GGTGACC CelII 3652 GCTCAGC GC^T[ACGT]AGC 4073 GCTCAGC DraIII 125 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 1274 CACCGTGTG EcoO65I 584 GGTCACC G^GT[ACGT]ACC 4275 GGTGACC EcoT22I 2981 ATGCAT ATGCA^T 5370 ATGCAT HaeII 1111 GGCGCT [GA]GCGC^[CT] 3219 GGCGCC MluNI 3156 TGGCCA TGG^CCA 4023 TGGCCA MscI 3156 TGGCCA TGG^CCA 4023 TGGCCA NsiI 2981 ATGCAT ATGCA^T 5370 ATGCAT PaeR7I 2834 CTCGAG C^TCGAG 3673 CTCGAG SmaI 458 CCCGGG CCC^GGG 772 CCCGGG XhoI 2834 CTCGAG C^TCGAG 3673 CTCGAG XmaCI 458 CCCGGG C^CCGGG 772 CCCGGG XmaI 458 CCCGGG C^CCGGG 772 CCCGGG XmnI 1898 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3031 GAAGATGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 127 enzymes) # ######################################### AccII AccIII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII AvrII BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlnI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MroI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII