##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
Acc65I AgeI AocI Aor51HI AscI
Asp718I AvaI AviII AvrII BalI
BbvCI BcgI BciVI BglI BlnI
BsaI BsiWI BsoBI BspDI BspLU11I
BsrDI BssHII BstBI Bsu36I ClaI
CpoI DraIII EagI EclXI Eco47III
Eco52I Eco81I EcoRV EcoT22I FseI
FspI KpnI KspI MluI MluNI
MscI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PaeR7I PinAI PmeI PshAI PvuI
PvuII RsrII SacII SalI SbfI
SfiI SfuI SgrAI SmaI SpeI
Sse8387I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 2691 TCCGGA T^CCGGA
AclI 862 AACGTT AA^CGTT
AhdI 2582 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2021 GGGCCC GGGCC^C
AseI 980 ATTAAT AT^TAAT
AsnI 980 ATTAAT AT^TAAT
AspEI 2582 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2951 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 22 GGATCC G^GATCC
BbeI 59 GGCGCC GGCGC^C
BglII 2249 AGATCT A^GATCT
BsaBI 2753 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2691 TCCGGA [AT]^CCGG[AT]
BseAI 2691 TCCGGA T^CCGGA
BsiEI 2652 CGGTCG CG[GA][CT]^CG
BsmBI 144 CGTCTC CGTCTC(1/5)
BspEI 2691 TCCGGA T^CCGGA
BspHI 552 TCATGA T^CATGA
BsrBI 406 CCGCTC CCGCTC(-3/-3)
BssSI 1304 CTCGTG CACGAG(-5/-1)
BstEII 2979 GGTCACC G^GT[ACGT]ACC
BstPI 2979 GGTCACC G^GT[ACGT]ACC
DsaI 2877 CCGTGG C^C[GA][CT]GG
Eam1105I 2582 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3251 CCTTTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2979 GGTCACC G^GT[ACGT]ACC
HgaI 99 GCGTC GACGC(5/10)
HpaI 4647 GTTAAC GTT^AAC
KasI 59 GGCGCC G^GCGCC
MamI 2753 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5069 CAATTG C^AATTG
MroI 2691 TCCGGA T^CCGGA
MspA1I 445 CCGCTG C[AC]G^C[GT]G
MunI 5069 CAATTG C^AATTG
NarI 59 GGCGCC GG^CGCC
PacI 1862 TTAATTAA TTAAT^TAA
PflFI 2951 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 4132 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 980 ATTAAT AT^TAAT
Psp1406I 862 AACGTT AA^CGTT
RcaI 552 TCATGA T^CATGA
SacI 16 GAGCTC GAGCT^C
SfoI 59 GGCGCC GGC^GCC
SnaBI 1160 TACGTA TAC^GTA
SphI 1314 GCATGC GCATG^C
StuI 3609 AGGCCT AGG^CCT
SwaI 4368 ATTTAAAT ATTT^AAAT
Tth111I 2951 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4204 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AatII 1235 GACGTC GACGT^C
2582 GACGTC
Asp700I 3198 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3843 GAACTTATTC
BbrPI 328 CACGTG CAC^GTG
5043 CACGTG
BcnI 224 CCGGG CC^[GC]GG
4047 CCGGG
BlpI 2338 GCTGAGC GC^T[ACGT]AGC
2698 GCTCAGC
Bpu1102I 2338 GCTGAGC GC^T[ACGT]AGC
2698 GCTCAGC
BsmFI 2225 GTCCC GGGAC(10/14)
4260 GTCCC
Bsp1407I 4489 TGTACA T^GTACA
5103 TGTACA
BspMI 2716 ACCTGC ACCTGC(4/8)
4281 ACCTGC
BsrFI 317 ACCGGC [GA]^CCGG[CT]
2650 GCCGGT
BsrGI 4489 TGTACA T^GTACA
5103 TGTACA
Bst1107I 2564 GTATAC GTA^TAC
3699 GTATAC
BstAPI 1632 GCATGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2123 GCAGAAGCTGC
BstZ17I 2564 GTATAC GTA^TAC
3699 GTATAC
CelII 2338 GCTGAGC GC^T[ACGT]AGC
2698 GCTCAGC
Cfr10I 317 ACCGGC [GA]^CCGG[CT]
2650 GCCGGT
DraII 2020 AGGGCCC [GA]G^G[ACGT]CC[CT]
4132 GGGTCCT
DrdI 176 GACTTAATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
434 GACACGCTGGTC
EcoO109I 2020 AGGGCCC [GA]G^G[ACGT]CC[CT]
4132 GGGTCCT
EcoRI 725 GAATTC G^AATTC
5089 GAATTC
FauI 2291 CCCGC CCCGC(4/6)
545 GCGGG
HaeII 50 GGCGCT [GA]GCGC^[CT]
59 GGCGCC
HindIII 389 AAGCTT A^AGCTT
4222 AAGCTT
NciI 224 CCGGG CC^[GC]GG
4047 CCGGG
NspI 1314 GCATGC [GA]CATG^[CT]
4033 GCATGT
PflMI 1321 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1442 CCACCGTTTGG
PmaCI 328 CACGTG CAC^GTG
5043 CACGTG
PmlI 328 CACGTG CAC^GTG
5043 CACGTG
PstI 2170 CTGCAG CTGCA^G
2854 CTGCAG
SapI 111 GCTCTTC GCTCTTC(1/4)
3522 GCTCTTC
ScaI 2180 AGTACT AGT^ACT
4173 AGTACT
SexAI 2352 ACCAGGT A^CC[AT]GGT
3561 ACCTGGT
SspBI 4489 TGTACA T^GTACA
5103 TGTACA
TaqI 2518 TCGA T^CGA
2655 TCGA
TthHB8I 2518 TCGA T^CGA
2655 TCGA
Van91I 1321 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1442 CCACCGTTTGG
XmnI 3198 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3843 GAACTTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AflIII AluI Alw44I
AlwI AlwNI ApaLI ApoI AspHI
AvaII BanI BanII BbsI BbvI
BclI BfaI BfrI BmyI BpmI
BpuAI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EaeI EarI EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII