##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AcyI AflII AgeI AhdI
Alw44I ApaLI AscI AspEI AviII
AvrII BbeI BfrI BglII BlnI
BsaHI BsiWI BsmBI BspDI BspLU11I
BssHII BstBI ClaI CpoI Eam1105I
EarI FseI FspI Hin1I HindIII
KasI Ksp632I KspI MluI NarI
NheI NruI NspV PacI PaeR7I
PinAI PmeI PvuI RsrII SacII
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 1291 TCCGGA T^CCGGA
AclI 4525 AACGTT AA^CGTT
Aor51HI 746 AGCGCT AGC^GCT
AseI 1762 ATTAAT AT^TAAT
AsnI 1762 ATTAAT AT^TAAT
Asp700I 4962 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3343 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 1781 TGATCA T^GATCA
BsaBI 2372 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1291 TCCGGA T^CCGGA
BsiEI 2 CGGCCG CG[GA][CT]^CG
BsmI 2272 GAATGC GAATGC(1/-1)
BspEI 1291 TCCGGA T^CCGGA
BspHI 4061 TCATGA T^CATGA
BsrBI 79 CCGCTC CCGCTC(-3/-3)
BssSI 3588 CTCGTG CACGAG(-5/-1)
Bst1107I 3238 GTATAC GTA^TAC
BstXI 104 CCAGTCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3238 GTATAC GTA^TAC
DraI 4505 TTTAAA TTT^AAA
DrdI 2397 GACTCCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2 CGGCCG C^GGCCG
EclXI 2 CGGCCG C^GGCCG
Eco47III 746 AGCGCT AGC^GCT
Eco52I 2 CGGCCG C^GGCCG
EcoRI 2565 GAATTC G^AATTC
FbaI 1781 TGATCA T^GATCA
MamI 2372 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5003 CAATTG C^AATTG
MroI 1291 TCCGGA T^CCGGA
MunI 5003 CAATTG C^AATTG
NaeI 2749 GCCGGC GCC^GGC
NgoMIV 2749 GCCGGC G^CCGGC
NotI 1 GCGGCCGC GC^GGCCGC
PflFI 3343 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1762 ATTAAT AT^TAAT
Psp1406I 4525 AACGTT AA^CGTT
RcaI 4061 TCATGA T^CATGA
SacI 926 GAGCTC GAGCT^C
SalI 576 GTCGAC G^TCGAC
SmaI 52 CCCGGG CCC^GGG
SphI 3689 GCATGC GCATG^C
StuI 4380 AGGCCT AGG^CCT
Tth111I 3343 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 52 CCCGGG C^CCGGG
XmaI 52 CCCGGG C^CCGGG
XmnI 4962 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 209 GGTACC G^GTACC
248 GGTACC
AccII 39 CGCG CG^CG
1280 CGCG
AflIII 4683 ACGTGT A^C[GA][CT]GT
5147 ACGTGT
Asp718I 209 GGTACC G^GTACC
248 GGTACC
BbrPI 4682 CACGTG CAC^GTG
5146 CACGTG
BbsI 2293 GAAGAC GAAGAC(2/6)
4106 GAAGAC
BcgI 254 CGATGACCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2879 CGAATCTCATGC
BciVI 696 GTATCC GTATCC(6/5)
3712 GTATCC
BglI 1134 GCCACCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2711 GCCCCAACGGC
BlpI 1265 GCTCAGC GC^T[ACGT]AGC
3433 GCTCAGC
Bpu1102I 1265 GCTCAGC GC^T[ACGT]AGC
3433 GCTCAGC
BpuAI 2293 GAAGAC GAAGAC(2/6)
4106 GAAGAC
BsaAI 4682 CACGTG [CT]AC^GT[GA]
5146 CACGTG
BsaWI 1291 TCCGGA [AT]^CCGG[AT]
1634 ACCGGA
BsrFI 1556 ACCGGC [GA]^CCGG[CT]
2749 GCCGGC
BstAPI 3165 GCAGCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3378 GCAAAACATGC
BstEII 2728 GGTGACC G^GT[ACGT]ACC
4786 GGTGACC
BstPI 2728 GGTGACC G^GT[ACGT]ACC
4786 GGTGACC
BstUI 39 CGCG CG^CG
1280 CGCG
CelII 1265 GCTCAGC GC^T[ACGT]AGC
3433 GCTCAGC
Cfr10I 1556 ACCGGC [GA]^CCGG[CT]
2749 GCCGGC
EcoO65I 2728 GGTGACC G^GT[ACGT]ACC
4786 GGTGACC
EcoRV 3871 GATATC GAT^ATC
4830 GATATC
HaeII 746 AGCGCT [GA]GCGC^[CT]
3290 AGCGCC
HgaI 675 GACGC GACGC(5/10)
1281 GCGTC
HpaI 1884 GTTAAC GTT^AAC
4775 GTTAAC
KpnI 209 GGTACC GGTAC^C
248 GGTACC
MvnI 39 CGCG CG^CG
1280 CGCG
PmaCI 4682 CACGTG CAC^GTG
5146 CACGTG
PmlI 4682 CACGTG CAC^GTG
5146 CACGTG
PshAI 179 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2154 GACTCAGGTC
SmlI 773 CTCAAG C^T[CT][GA]AG
3985 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbvCI BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI Eco81I EcoNI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII