##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AccI AccII AclI AgeI
AhdI ApaI AscI Asp718I AspEI
AvaI AvaII AviII BamHI BbrPI
BglI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BsrBI BsrGI BssHII
Bst1107I BstBI BstUI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco52I
EcoRV FauI FseI FspI HgaI
HindIII HpaI KpnI KspI MfeI
MluI MunI MvnI NaeI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 1642 GACGTC GACGT^C
AccIII 1009 TCCGGA T^CCGGA
Alw44I 2798 GTGCAC G^TGCAC
Aor51HI 2155 AGCGCT AGC^GCT
ApaLI 2798 GTGCAC G^TGCAC
AseI 3545 ATTAAT AT^TAAT
AsnI 3545 ATTAAT AT^TAAT
AvrII 3116 CCTAGG C^CTAGG
BbeI 607 GGCGCC GGCGC^C
BciVI 3376 GGATAC GTATCC(6/5)
BclI 1747 TGATCA T^GATCA
BglII 3030 AGATCT A^GATCT
BlnI 3116 CCTAGG C^CTAGG
BlpI 3712 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 3712 GCTTAGC GC^T[ACGT]AGC
BsaAI 2147 CACGTA [CT]AC^GT[GA]
BsaBI 1760 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1009 TCCGGA [AT]^CCGG[AT]
BseAI 1009 TCCGGA T^CCGGA
BsmBI 559 CGTCTC CGTCTC(1/5)
BspEI 1009 TCCGGA T^CCGGA
BspLU11I 1154 ACATGT A^CATGT
BsrFI 532 ACCGGC [GA]^CCGG[CT]
BssSI 642 CACGAG CACGAG(-5/-1)
BstEII 2903 GGTTACC G^GT[ACGT]ACC
BstPI 2903 GGTTACC G^GT[ACGT]ACC
CelII 3712 GCTTAGC GC^T[ACGT]AGC
Cfr10I 532 ACCGGC [GA]^CCGG[CT]
DraII 2164 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 3472 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3905 GACTTTGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2155 AGCGCT AGC^GCT
EcoNI 3783 CCTCTTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2164 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 2903 GGTTACC G^GT[ACGT]ACC
EcoRI 1604 GAATTC G^AATTC
FbaI 1747 TGATCA T^GATCA
KasI 607 GGCGCC G^GCGCC
MamI 1760 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1009 TCCGGA T^CCGGA
NarI 607 GGCGCC GG^CGCC
NcoI 817 CCATGG C^CATGG
NheI 1649 GCTAGC G^CTAGC
PshAI 1506 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3545 ATTAAT AT^TAAT
PstI 3640 CTGCAG CTGCA^G
SacI 1924 GAGCTC GAGCT^C
SapI 2288 GAAGAGC GCTCTTC(1/4)
SfoI 607 GGCGCC GGC^GCC
SspI 74 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 607 GGCGCC G[GA]^CG[CT]C
1642 GACGTC
AflII 55 CTTAAG C^TTAAG
2073 CTTAAG
AflIII 1154 ACATGT A^C[GA][CT]GT
2146 ACACGT
Asp700I 1268 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2288 GAAGAGCTTC
AspHI 1924 GAGCTC G[AT]GC[AT]^C
2798 GTGCAC
AspI 236 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2127 GACTGGGTC
BanI 354 GGTGCC G^G[CT][GA]CC
607 GGCGCC
BanII 1924 GAGCTC G[GA]GC[CT]^C
3107 GAGCCC
BbsI 2133 GTCTTC GAAGAC(2/6)
3283 GTCTTC
BbvCI 325 CCTCAGC CCTCAGC(-5/-2)
2759 CCTCAGC
BcgI 314 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
913 GCAACCTTATCG
BfrI 55 CTTAAG C^TTAAG
2073 CTTAAG
BpuAI 2133 GTCTTC GAAGAC(2/6)
3283 GTCTTC
BsaHI 607 GGCGCC G[GA]^CG[CT]C
1642 GACGTC
BsgI 4 GTGCAG GTGCAG(16/14)
263 GTGCAG
BsiHKAI 1924 GAGCTC G[AT]GC[AT]^C
2798 GTGCAC
BspHI 2457 TCATGA T^CATGA
3336 TCATGA
BstYI 1880 GGATCT [GA]^GATC[CT]
3030 AGATCT
CfoI 608 GCGC GCG^C
2156 GCGC
EcoT22I 2375 ATGCAT ATGCA^T
3549 ATGCAT
HaeII 607 GGCGCC [GA]GCGC^[CT]
2155 AGCGCT
HhaI 608 GCGC GCG^C
2156 GCGC
Hin1I 607 GGCGCC G[GA]^CG[CT]C
1642 GACGTC
HinP1I 608 GCGC G^CGC
2156 GCGC
MflI 1880 GGATCT [GA]^GATC[CT]
3030 AGATCT
NsiI 2375 ATGCAT ATGCA^T
3549 ATGCAT
NspI 1022 ACATGC [GA]CATG^[CT]
1154 ACATGT
PflFI 236 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2127 GACTGGGTC
RcaI 2457 TCATGA T^CATGA
3336 TCATGA
ScaI 2215 AGTACT AGT^ACT
2655 AGTACT
TaqI 2047 TCGA T^CGA
2571 TCGA
Tth111I 236 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2127 GACTGGGTC
TthHB8I 2047 TCGA T^CGA
2571 TCGA
XhoII 1880 GGATCT [GA]^GATC[CT]
3030 AGATCT
XmnI 1268 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2288 GAAGAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI BalI BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstXI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraI DsaI EaeI EarI Eco81I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI