##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AccIII AclI AhdI AseI
AsnI AspEI AspI AvrII BamHI
BbrPI BciVI BclI BlnI BsaBI
BseAI BsiWI BsmBI BsmI BspDI
BspEI BspLU11I BsrDI BstBI ClaI
CpoI Eam1105I EcoNI EcoRV FbaI
FseI MamI MfeI MroI MunI
NcoI NdeI NheI NotI NruI
NspV PacI PflFI PleI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI RsrII SalI SbfI ScaI
SfiI SfuI SpeI Sse8387I Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 2525 GGTACC G^GTACC
AflII 2922 CTTAAG C^TTAAG
AgeI 3433 ACCGGT A^CCGGT
AocI 2455 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2728 AGCGCT AGC^GCT
Asp700I 952 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2525 GGTACC G^GTACC
AviII 1950 TGCGCA TGC^GCA
BcgI 717 GCACGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2922 CTTAAG C^TTAAG
BglII 4387 AGATCT A^GATCT
BsaAI 1363 TACGTA [CT]AC^GT[GA]
BsaWI 3433 ACCGGT [AT]^CCGG[AT]
Bst1107I 4063 GTATAC GTA^TAC
BstAPI 889 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 4063 GTATAC GTA^TAC
Bsu36I 2455 CCTGAGG CC^T[ACGT]AGG
DraIII 1239 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2141 GACTTCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2728 AGCGCT AGC^GCT
Eco81I 2455 CCTGAGG CC^T[ACGT]AGG
EcoRI 4285 GAATTC G^AATTC
EcoT14I 1254 CCAAGG C^C[AT][AT]GG
EcoT22I 3800 ATGCAT ATGCA^T
FspI 1950 TGCGCA TGC^GCA
HinfI 158 GAATC G^A[ACGT]TC
HpaI 4634 GTTAAC GTT^AAC
KpnI 2525 GGTACC GGTAC^C
MluI 2610 ACGCGT A^CGCGT
NsiI 3800 ATGCAT ATGCA^T
PaeR7I 2380 CTCGAG C^TCGAG
PinAI 3433 ACCGGT A^CCGGT
PpuMI 4005 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 2278 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfcI 2280 CTACAG C^T[GA][CT]AG
SnaBI 1363 TACGTA TAC^GTA
StyI 1254 CCAAGG C^C[AT][AT]GG
SwaI 2591 ATTTAAAT ATTT^AAAT
TfiI 158 GAATC G^A[AT]TC
XhoI 2380 CTCGAG C^TCGAG
XmnI 952 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 1038 ACACGT A^C[GA][CT]GT
2610 ACGCGT
AscI 113 GGCGCGCC GG^CGCGCC
496 GGCGCGCC
BsaI 2289 GAGACC GGTCTC(1/5)
260 GGTCTC
BspMI 753 ACCTGC ACCTGC(4/8)
3824 GCAGGT
BstEII 3121 GGTCACC G^GT[ACGT]ACC
3459 GGTCACC
BstPI 3121 GGTCACC G^GT[ACGT]ACC
3459 GGTCACC
BstYI 2205 GGATCT [GA]^GATC[CT]
4387 AGATCT
EcoO65I 3121 GGTCACC G^GT[ACGT]ACC
3459 GGTCACC
HincII 479 GTTGAC GT[CT]^[GA]AC
4634 GTTAAC
HindII 479 GTTGAC GT[CT]^[GA]AC
4634 GTTAAC
KspI 41 CCGCGG CCGC^GG
171 CCGCGG
MflI 2205 GGATCT [GA]^GATC[CT]
4387 AGATCT
PflMI 1210 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1596 CCATCTGGTGG
PvuII 503 CAGCTG CAG^CTG
2047 CAGCTG
SacI 836 GAGCTC GAGCT^C
3224 GAGCTC
SacII 41 CCGCGG CCGC^GG
171 CCGCGG
SapI 1348 GCTCTTC GCTCTTC(1/4)
1882 GCTCTTC
SphI 3798 GCATGC GCATG^C
3802 GCATGC
SspI 3909 AATATT AAT^ATT
4470 AATATT
StuI 2022 AGGCCT AGG^CCT
2314 AGGCCT
Van91I 1210 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1596 CCATCTGGTGG
XhoII 2205 GGATCT [GA]^GATC[CT]
4387 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspHI
BsrBI BsrFI BsrGI BsrI BssHII
BssKI BssSI BstF5I BstNI BstUI
BstXI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI RcaI RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfoI
SgrAI SmaI SmlI SspBI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XmaCI XmaI