##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AspEI AvaI
AviII AvrII BalI BamHI BbrPI
BclI BfrI BglI BlnI BseAI
BsgI BsiEI BsiWI BsmBI BsoBI
BspEI BspLU11I BspMI BssHII Bst1107I
BstEII BstPI BstZ17I Bsu36I CpoI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV FbaI FspI
HgaI HindIII KspI MfeI MluI
MluNI MroI MscI MunI NdeI
NheI NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SgrAI SmaI
SnaBI SpeI Sse8387I SspI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 1893 GGTACC G^GTACC
AccII 48 CGCG CG^CG
AcyI 153 GGCGCC G[GA]^CG[CT]C
AflIII 2135 ACACGT A^C[GA][CT]GT
AseI 2672 ATTAAT AT^TAAT
AsnI 2672 ATTAAT AT^TAAT
Asp718I 1893 GGTACC G^GTACC
AspI 817 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 153 GGCGCC GGCGC^C
BcgI 3806 GCATGCTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3242 GTATCC GTATCC(6/5)
BglII 3715 AGATCT A^GATCT
BsaAI 2136 CACGTA [CT]AC^GT[GA]
BsaBI 3710 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 153 GGCGCC G[GA]^CG[CT]C
BsaI 3646 GAGACC GGTCTC(1/5)
BsaWI 114 ACCGGA [AT]^CCGG[AT]
BspDI 2257 ATCGAT AT^CGAT
BsrBI 56 CCGCTC CCGCTC(-3/-3)
BssSI 3166 CACGAG CACGAG(-5/-1)
BstAPI 2462 GCAAGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 673 TTCGAA TT^CGAA
BstUI 48 CGCG CG^CG
BstXI 3098 CCATGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 2257 ATCGAT AT^CGAT
DraIII 1509 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 377 TGGCCG [CT]^GGCC[GA]
EcoNI 1785 CCTGTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4667 GAATTC G^AATTC
EcoT22I 2904 ATGCAT ATGCA^T
FseI 378 GGCCGGCC GGCCGG^CC
HaeII 153 GGCGCC [GA]GCGC^[CT]
Hin1I 153 GGCGCC G[GA]^CG[CT]C
HpaI 2348 GTTAAC GTT^AAC
KasI 153 GGCGCC G^GCGCC
KpnI 1893 GGTACC GGTAC^C
MamI 3710 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 48 CGCG CG^CG
NaeI 379 GCCGGC GCC^GGC
NarI 153 GGCGCC GG^CGCC
NgoMIV 379 GCCGGC G^CCGGC
NsiI 2904 ATGCAT ATGCA^T
NspV 673 TTCGAA TT^CGAA
PflFI 817 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2672 ATTAAT AT^TAAT
SacI 1727 GAGCTC GAGCT^C
ScaI 3272 AGTACT AGT^ACT
SfoI 153 GGCGCC GGC^GCC
SfuI 673 TTCGAA TT^CGAA
SmlI 1732 CTCAAG C^T[CT][GA]AG
SwaI 3212 ATTTAAAT ATTT^AAAT
Tth111I 817 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2153 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AspHI 1727 GAGCTC G[AT]GC[AT]^C
1825 GTGCTC
BanI 153 GGCGCC G^G[CT][GA]CC
1893 GGTACC
BanII 1727 GAGCTC G[GA]GC[CT]^C
3840 GAGCCC
BbvCI 169 GCTGAGG CCTCAGC(-5/-2)
1866 GCTGAGG
BcnI 69 CCCGG CC^[GC]GG
157 CCCGG
BlpI 3837 GCTGAGC GC^T[ACGT]AGC
3957 GCTGAGC
Bpu1102I 3837 GCTGAGC GC^T[ACGT]AGC
3957 GCTGAGC
BsiHKAI 1727 GAGCTC G[AT]GC[AT]^C
1825 GTGCTC
BsmFI 595 GGGAC GGGAC(10/14)
3073 GTCCC
BsmI 686 GAATGC GAATGC(1/-1)
4018 GAATGC
BspHI 943 TCATGA T^CATGA
1274 TCATGA
BsrFI 379 GCCGGC [GA]^CCGG[CT]
1896 ACCGGC
BstYI 2387 GGATCT [GA]^GATC[CT]
3715 AGATCT
CelII 3837 GCTGAGC GC^T[ACGT]AGC
3957 GCTGAGC
CfoI 47 GCGC GCG^C
154 GCGC
Cfr10I 379 GCCGGC [GA]^CCGG[CT]
1896 ACCGGC
DrdI 878 GACGGGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
891 GACTCCCAGGTC
HhaI 47 GCGC GCG^C
154 GCGC
HinP1I 47 GCGC G^CGC
154 GCGC
MflI 2387 GGATCT [GA]^GATC[CT]
3715 AGATCT
NciI 69 CCCGG CC^[GC]GG
157 CCCGG
NcoI 3640 CCATGG C^CATGG
3913 CCATGG
PpuMI 243 AGGACCT [GA]G^G[AT]CC[CT]
3071 AGGTCCC
RcaI 943 TCATGA T^CATGA
1274 TCATGA
SapI 425 GAAGAGC GCTCTTC(1/4)
466 GAAGAGC
SphI 2906 GCATGC GCATG^C
3806 GCATGC
StuI 2836 AGGCCT AGG^CCT
4082 AGGCCT
XhoII 2387 GGATCT [GA]^GATC[CT]
3715 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AvaII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I Bsp1407I BsrDI BsrGI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI DraII DsaI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XmnI