##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccIII AgeI Alw44I AocI
ApaI ApaLI AscI AviII BbeI
BbrPI BbvCI BcgI BlpI Bpu1102I
BsaWI BseAI BsiWI BsmBI BspEI
BsrBI BssHII Bst1107I BstAPI BstEII
BstPI BstZ17I Bsu36I CelII CpoI
DraII DraIII DrdI EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRV FspI
KasI Ksp632I MfeI MroI MunI
NarI NheI NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PvuI PvuII RsrII
SacI SapI SexAI SfiI SfoI
SgrAI SnaBI SpeI SphI StuI
XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 72 enzymes) #
###################################
Acc65I 1308 GGTACC G^GTACC
AccI 879 GTCGAC GT^[AC][GT]AC
AclI 3330 AACGTT AA^CGTT
AcyI 2239 GGCGTC G[GA]^CG[CT]C
AflII 2962 CTTAAG C^TTAAG
AhdI 1511 GACCAATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 490 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 270 AGCGCT AGC^GCT
Asp700I 3706 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1308 GGTACC G^GTACC
AspEI 1511 GACCAATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 81 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 189 CCCGGG C^[CT]CG[GA]G
AvrII 1392 CCTAGG C^CTAGG
BalI 3898 TGGCCA TGG^CCA
BamHI 1107 GGATCC G^GATCC
BanI 1308 GGTACC G^G[CT][GA]CC
BclI 1868 TGATCA T^GATCA
BfrI 2962 CTTAAG C^TTAAG
BglI 229 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1447 AGATCT A^GATCT
BlnI 1392 CCTAGG C^CTAGG
BsaAI 1053 TACGTG [CT]AC^GT[GA]
BsaBI 2209 GATTAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2239 GGCGTC G[GA]^CG[CT]C
BsgI 113 CTGCAC GTGCAG(16/14)
BsiEI 1637 CGGCCG CG[GA][CT]^CG
BsmI 1949 GAATGC GAATGC(1/-1)
BsoBI 189 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2587 TGTACA T^GTACA
BspDI 1385 ATCGAT AT^CGAT
BspMI 2318 GCAGGT ACCTGC(4/8)
BsrGI 2587 TGTACA T^GTACA
BssSI 180 CTCGTG CACGAG(-5/-1)
BstBI 336 TTCGAA TT^CGAA
ClaI 1385 ATCGAT AT^CGAT
EagI 1637 CGGCCG C^GGCCG
Eam1105I 1511 GACCAATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1637 CGGCCG C^GGCCG
Eco47III 270 AGCGCT AGC^GCT
Eco52I 1637 CGGCCG C^GGCCG
EcoRI 3384 GAATTC G^AATTC
EcoT22I 1890 ATGCAT ATGCA^T
FbaI 1868 TGATCA T^GATCA
FseI 224 GGCCGGCC GGCCGG^CC
HaeII 270 AGCGCT [GA]GCGC^[CT]
Hin1I 2239 GGCGTC G[GA]^CG[CT]C
HpaI 1757 GTTAAC GTT^AAC
KpnI 1308 GGTACC GGTAC^C
KspI 127 CCGCGG CCGC^GG
MamI 2209 GATTAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 16 ACGCGT A^CGCGT
MluNI 3898 TGGCCA TGG^CCA
MscI 3898 TGGCCA TGG^CCA
NsiI 1890 ATGCAT ATGCA^T
NspV 336 TTCGAA TT^CGAA
PflFI 81 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3292 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 3330 AACGTT AA^CGTT
PstI 69 CTGCAG CTGCA^G
SacII 127 CCGCGG CCGC^GG
SalI 879 GTCGAC G^TCGAC
SbfI 68 CCTGCAGG CCTGCA^GG
SfuI 336 TTCGAA TT^CGAA
SmaI 189 CCCGGG CCC^GGG
Sse8387I 68 CCTGCAGG CCTGCA^GG
SspBI 2587 TGTACA T^GTACA
Tth111I 81 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3292 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 189 CCCGGG C^CCGGG
XmaI 189 CCCGGG C^CCGGG
XmnI 3706 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AvaII 543 GGACC G^G[AT]CC
630 GGACC
BanII 75 GAGCCC G[GA]GC[CT]^C
185 GAGCCC
BbsI 2772 GAAGAC GAAGAC(2/6)
901 GTCTTC
BciVI 2016 GTATCC GTATCC(6/5)
3288 GTATCC
BpuAI 2772 GAAGAC GAAGAC(2/6)
901 GTCTTC
BsrDI 776 CATTGC GCAATG(2/0)
3049 GCAATG
CfoI 109 GCGC GCG^C
271 GCGC
HhaI 109 GCGC GCG^C
271 GCGC
HinP1I 109 GCGC G^CGC
271 GCGC
HindIII 907 AAGCTT A^AGCTT
3304 AAGCTT
NaeI 40 GCCGGC GCC^GGC
225 GCCGGC
NgoMIV 40 GCCGGC G^CCGGC
225 GCCGGC
SfcI 69 CTGCAG C^T[GA][CT]AG
3541 CTATAG
SwaI 2640 ATTTAAAT ATTT^AAAT
2684 ATTTAAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
AspHI BbvI BcnI BfaI BmyI
BpmI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BspLU11I BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I Cfr10I Cfr13I DdeI DpnII
DraI DsaI EaeI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PleI PshBI
RcaI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII