##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AgeI AhdI AscI
Asp718I AspEI AspI AviII BbsI
BcgI BciVI BclI BglI BlpI
Bpu1102I BpuAI BsaBI BsiEI BsiWI
BsmBI BspDI BspLU11I BsrBI BsrFI
BssSI BstBI BstXI CelII Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EarI EclXI Eco52I EcoRI
EcoRV FbaI FseI FspI KpnI
Ksp632I KspI MamI NaeI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PvuI RsrII SacII SalI SapI
SbfI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI Tth111I XbaI
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 65 enzymes) #
###################################
AccI 790 GTATAC GT^[AC][GT]AC
AccIII 143 TCCGGA T^CCGGA
AclI 1096 AACGTT AA^CGTT
AcyI 351 GGCGCC G[GA]^CG[CT]C
AflII 2490 CTTAAG C^TTAAG
Alw44I 208 GTGCAC G^TGCAC
AocI 2076 CCTAAGG CC^T[ACGT]AGG
ApaLI 208 GTGCAC G^TGCAC
Asp700I 2955 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 436 GGACC G^G[AT]CC
AvrII 1669 CCTAGG C^CTAGG
BalI 413 TGGCCA TGG^CCA
BanI 351 GGCGCC G^G[CT][GA]CC
BbeI 351 GGCGCC GGCGC^C
BbrPI 2676 CACGTG CAC^GTG
BfrI 2490 CTTAAG C^TTAAG
BlnI 1669 CCTAGG C^CTAGG
BsaAI 2676 CACGTG [CT]AC^GT[GA]
BsaHI 351 GGCGCC G[GA]^CG[CT]C
BsaWI 143 TCCGGA [AT]^CCGG[AT]
BseAI 143 TCCGGA T^CCGGA
BsmI 1626 GAATGC GAATGC(1/-1)
BspEI 143 TCCGGA T^CCGGA
BspHI 811 TCATGA T^CATGA
BssHII 126 GCGCGC G^CGCGC
Bst1107I 790 GTATAC GTA^TAC
BstAPI 210 GCACTTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2071 GGTGACC G^GT[ACGT]ACC
BstPI 2071 GGTGACC G^GT[ACGT]ACC
BstZ17I 790 GTATAC GTA^TAC
Bsu36I 2076 CCTAAGG CC^T[ACGT]AGG
DraIII 1353 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2076 CCTAAGG CC^T[ACGT]AGG
EcoO65I 2071 GGTGACC G^GT[ACGT]ACC
EcoT22I 509 ATGCAT ATGCA^T
HgaI 2063 GACGC GACGC(5/10)
Hin1I 351 GGCGCC G[GA]^CG[CT]C
HindIII 1516 AAGCTT A^AGCTT
HpaI 1099 GTTAAC GTT^AAC
KasI 351 GGCGCC G^GCGCC
MfeI 1995 CAATTG C^AATTG
MluI 2064 ACGCGT A^CGCGT
MluNI 413 TGGCCA TGG^CCA
MroI 143 TCCGGA T^CCGGA
MscI 413 TGGCCA TGG^CCA
MunI 1995 CAATTG C^AATTG
NarI 351 GGCGCC GG^CGCC
NcoI 2307 CCATGG C^CATGG
NheI 758 GCTAGC G^CTAGC
NsiI 509 ATGCAT ATGCA^T
NspI 1113 GCATGC [GA]CATG^[CT]
PleI 56 GAGTC GAGTC(4/5)
PmaCI 2676 CACGTG CAC^GTG
PmlI 2676 CACGTG CAC^GTG
PshAI 1641 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1096 AACGTT AA^CGTT
RcaI 811 TCATGA T^CATGA
SacI 1161 GAGCTC GAGCT^C
ScaI 1181 AGTACT AGT^ACT
SexAI 1354 ACCAGGT A^CC[AT]GGT
SfoI 351 GGCGCC GGC^GCC
SphI 1113 GCATGC GCATG^C
SspI 547 AATATT AAT^ATT
StuI 859 AGGCCT AGG^CCT
XmnI 2955 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 2064 ACGCGT A^C[GA][CT]GT
2398 ACGTGT
AlwNI 494 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2475 CAGTGACTG
Aor51HI 94 AGCGCT AGC^GCT
1586 AGCGCT
ApaI 106 GGGCCC GGGCC^C
2446 GGGCCC
BamHI 32 GGATCC G^GATCC
1969 GGATCC
BbvCI 322 CCTCAGC CCTCAGC(-5/-2)
1684 CCTCAGC
BglII 443 AGATCT A^GATCT
1393 AGATCT
BsaI 1171 GAGACC GGTCTC(1/5)
423 GGTCTC
BseRI 1164 CTCCTC GAGGAG(10/8)
1782 CTCCTC
BsgI 261 GTGCAG GTGCAG(16/14)
2472 GTGCAG
Bsp1407I 2177 TGTACA T^GTACA
2551 TGTACA
BsrDI 1188 GCAATG GCAATG(2/0)
1547 GCAATG
BsrGI 2177 TGTACA T^GTACA
2551 TGTACA
DsaI 1973 CCACGG C^C[GA][CT]GG
2307 CCATGG
EaeI 413 TGGCCA [CT]^GGCC[GA]
2005 TGGCCG
Eco47III 94 AGCGCT AGC^GCT
1586 AGCGCT
EcoNI 1349 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1523 CCTCCGCAAGG
HincII 576 GTTGAC GT[CT]^[GA]AC
1099 GTTAAC
HindII 576 GTTGAC GT[CT]^[GA]AC
1099 GTTAAC
MspA1I 136 CAGCTG C[AC]G^C[GT]G
461 CAGCTG
PstI 2114 CTGCAG CTGCA^G
2292 CTGCAG
PvuII 136 CAGCTG CAG^CTG
461 CAGCTG
SmaI 60 CCCGGG CCC^GGG
109 CCCGGG
SspBI 2177 TGTACA T^GTACA
2551 TGTACA
TaqI 427 TCGA T^CGA
1399 TCGA
TthHB8I 427 TCGA T^CGA
1399 TCGA
XmaCI 60 CCCGGG C^CCGGG
109 CCCGGG
XmaI 60 CCCGGG C^CCGGG
109 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI AspHI
AvaI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PshBI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XhoII