##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BamHI BbrPI BciVI BclI BfrI
BlnI BsaAI BsaBI BsiWI BsmI
BspDI BspLU11I BsrDI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I ClaI
CpoI DraI Eam1105I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HpaI KpnI MamI MfeI
MluI MseI MslI MunI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SalI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI SspI StuI SwaI TfiI
Tru9I Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 602 TCCGGA T^CCGGA
AcsI 1656 GAATTT [GA]^AATT[CT]
AocI 2284 CCTGAGG CC^T[ACGT]AGG
Aor51HI 753 AGCGCT AGC^GCT
ApaI 1977 GGGCCC GGGCC^C
ApoI 1656 GAATTT [GA]^AATT[CT]
Asp700I 513 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 262 TGGCCA TGG^CCA
BfaI 205 CTAG C^TAG
BglI 2103 GCCGTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 676 AGATCT A^GATCT
BseAI 602 TCCGGA T^CCGGA
BsiEI 2521 CGGCCG CG[GA][CT]^CG
BsmBI 1920 GAGACG CGTCTC(1/5)
Bsp1407I 2057 TGTACA T^GTACA
BspEI 602 TCCGGA T^CCGGA
BspHI 580 TCATGA T^CATGA
BsrGI 2057 TGTACA T^GTACA
BssHII 62 GCGCGC G^CGCGC
BssSI 2379 CTCGTG CACGAG(-5/-1)
BstYI 676 AGATCT [GA]^GATC[CT]
Bsu36I 2284 CCTGAGG CC^T[ACGT]AGG
DrdI 1299 GACAGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2521 CGGCCG C^GGCCG
EclXI 2521 CGGCCG C^GGCCG
Eco47III 753 AGCGCT AGC^GCT
Eco52I 2521 CGGCCG C^GGCCG
Eco81I 2284 CCTGAGG CC^T[ACGT]AGG
HindIII 1460 AAGCTT A^AGCTT
KspI 402 CCGCGG CCGC^GG
MaeI 205 CTAG C^TAG
MflI 676 AGATCT [GA]^GATC[CT]
MluNI 262 TGGCCA TGG^CCA
MroI 602 TCCGGA T^CCGGA
MscI 262 TGGCCA TGG^CCA
NaeI 1925 GCCGGC GCC^GGC
NcoI 2417 CCATGG C^CATGG
NgoMIV 1925 GCCGGC G^CCGGC
PflMI 2162 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 580 TCATGA T^CATGA
SacII 402 CCGCGG CCGC^GG
SbfI 1196 CCTGCAGG CCTGCA^GG
Sse8387I 1196 CCTGCAGG CCTGCA^GG
SspBI 2057 TGTACA T^GTACA
Van91I 2162 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 676 AGATCT [GA]^GATC[CT]
XmnI 513 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 927 GTGCAC G^TGCAC
1731 GTGCAC
ApaLI 927 GTGCAC G^TGCAC
1731 GTGCAC
AviII 898 TGCGCA TGC^GCA
913 TGCGCA
BbsI 513 GAAGAC GAAGAC(2/6)
1308 GTCTTC
BbvCI 489 GCTGAGG CCTCAGC(-5/-2)
1416 GCTGAGG
BcgI 1652 CGACGAATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1681 GCAGCCTCTTCG
BlpI 948 GCTGAGC GC^T[ACGT]AGC
2027 GCTCAGC
Bpu1102I 948 GCTGAGC GC^T[ACGT]AGC
2027 GCTCAGC
BpuAI 513 GAAGAC GAAGAC(2/6)
1308 GTCTTC
BsaI 285 GAGACC GGTCTC(1/5)
1581 GAGACC
BsaWI 602 TCCGGA [AT]^CCGG[AT]
1891 ACCGGA
BsrI 419 CCAGT ACTGG(1/-1)
1431 CCAGT
CelII 948 GCTGAGC GC^T[ACGT]AGC
2027 GCTCAGC
DraIII 1019 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
2544 CACACGGTG
FspI 898 TGCGCA TGC^GCA
913 TGCGCA
HinfI 69 GAGTC G^A[ACGT]TC
2014 GAGTC
PleI 69 GAGTC GAGTC(4/5)
2014 GAGTC
PvuII 1881 CAGCTG CAG^CTG
2536 CAGCTG
SacI 771 GAGCTC GAGCT^C
1335 GAGCTC
SapI 527 GAAGAGC GCTCTTC(1/4)
905 GCTCTTC
SexAI 1020 ACCTGGT A^CC[AT]GGT
1344 ACCAGGT
SmaI 1569 CCCGGG CCC^GGG
2077 CCCGGG
SmlI 312 CTCAAG C^T[CT][GA]AG
1665 CTTGAG
Tsp509I 1657 AATT ^AATT
2580 AATT
XmaCI 1569 CCCGGG C^CCGGG
2077 CCCGGG
XmaI 1569 CCCGGG C^CCGGG
2077 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BssKI BstAPI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI KasI Ksp632I
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XcmI