##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AclI AgeI AocI Aor51HI AscI
AviII AvrII BamHI BbvCI BclI
BlnI BlpI Bpu1102I BsiWI BsmBI
BspDI BspLU11I BsrBI BssSI Bst1107I
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DraIII Eco47III Eco81I EcoRI
FbaI FspI HgaI KspI MluI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmeI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 114 GACGTC GACGT^C
Acc65I 339 GGTACC G^GTACC
AccI 2492 GTCTAC GT^[AC][GT]AC
AccIII 134 TCCGGA T^CCGGA
AflII 1982 CTTAAG C^TTAAG
AflIII 1437 ACGTGT A^C[GA][CT]GT
AhdI 671 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1534 GTGCAC G^TGCAC
ApaLI 1534 GTGCAC G^TGCAC
AseI 3626 ATTAAT AT^TAAT
AsnI 3626 ATTAAT AT^TAAT
Asp718I 339 GGTACC G^GTACC
AspEI 671 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1648 GGCGCC GGCGC^C
BbrPI 1436 CACGTG CAC^GTG
BcgI 3081 CGAGCATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1094 GGATAC GTATCC(6/5)
BfrI 1982 CTTAAG C^TTAAG
BglI 1485 GCCAGAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2983 AGATCT A^GATCT
BsaBI 997 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3064 GGTCTC GGTCTC(1/5)
BseAI 134 TCCGGA T^CCGGA
BsiEI 20 CGGCCG CG[GA][CT]^CG
Bsp1407I 3471 TGTACA T^GTACA
BspEI 134 TCCGGA T^CCGGA
BspMI 2199 ACCTGC ACCTGC(4/8)
BsrGI 3471 TGTACA T^GTACA
BssHII 83 GCGCGC G^CGCGC
BstEII 412 GGTGACC G^GT[ACGT]ACC
BstPI 412 GGTGACC G^GT[ACGT]ACC
DrdI 2366 GACATGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 20 CGGCCG C^GGCCG
Eam1105I 671 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 20 CGGCCG C^GGCCG
Eco52I 20 CGGCCG C^GGCCG
EcoO65I 412 GGTGACC G^GT[ACGT]ACC
EcoRV 1429 GATATC GAT^ATC
EcoT22I 1773 ATGCAT ATGCA^T
FseI 171 GGCCGGCC GGCCGG^CC
HaeII 1648 GGCGCC [GA]GCGC^[CT]
HindIII 3441 AAGCTT A^AGCTT
HpaI 878 GTTAAC GTT^AAC
KasI 1648 GGCGCC G^GCGCC
KpnI 339 GGTACC GGTAC^C
MamI 997 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3225 CAATTG C^AATTG
MroI 134 TCCGGA T^CCGGA
MunI 3225 CAATTG C^AATTG
NarI 1648 GGCGCC GG^CGCC
NsiI 1773 ATGCAT ATGCA^T
PmaCI 1436 CACGTG CAC^GTG
PmlI 1436 CACGTG CAC^GTG
PshAI 415 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3626 ATTAAT AT^TAAT
SfoI 1648 GGCGCC GGC^GCC
SphI 3084 GCATGC GCATG^C
SspBI 3471 TGTACA T^GTACA
StuI 922 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 114 GACGTC G[GA]^CG[CT]C
1648 GGCGCC
AlwNI 1662 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
2792 CAGTTGCTG
ApaI 829 GGGCCC GGGCC^C
1574 GGGCCC
AspI 976 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2486 GACCGAGTC
BpmI 2674 CTGGAG CTGGAG(16/14)
2798 CTGGAG
BsaAI 1436 CACGTG [CT]AC^GT[GA]
1531 TACGTG
BsaHI 114 GACGTC G[GA]^CG[CT]C
1648 GGCGCC
BsaWI 134 TCCGGA [AT]^CCGG[AT]
1425 ACCGGA
BsrDI 2440 CATTGC GCAATG(2/0)
3105 CATTGC
BstAPI 43 GCACTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
584 GCACATCATGC
BstXI 2025 CCATGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2898 CCATCAGTGTGG
Hin1I 114 GACGTC G[GA]^CG[CT]C
1648 GGCGCC
HincII 845 GTCAAC GT[CT]^[GA]AC
878 GTTAAC
HindII 845 GTCAAC GT[CT]^[GA]AC
878 GTTAAC
NspI 2352 GCATGT [GA]CATG^[CT]
3084 GCATGC
PflFI 976 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2486 GACCGAGTC
SmlI 1982 CTTAAG C^T[CT][GA]AG
2148 CTCAAG
SspI 1597 AATATT AAT^ATT
3531 AATATT
Tth111I 976 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2486 GACCGAGTC
XcmI 1461 CCAAAGAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3551 CCATATTTGACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PleI
PpuMI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmaCI XmaI XmnI