##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AscI Asp718I AviII BfrI BsaWI
BseAI BsiWI BspDI BspEI Bst1107I
BstBI BstZ17I ClaI DraIII EcoRI
EcoRV FseI FspI HindIII KpnI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NspV PacI
PinAI PmeI Psp1406I PvuI SalI
SbfI SfuI SgrAI SpeI Sse8387I
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AhdI 6644 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3473 AGCGCT AGC^GCT
AseI 4000 ATTAAT AT^TAAT
AsnI 4000 ATTAAT AT^TAAT
AspEI 6644 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 139 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1147 CCTAGG C^CTAGG
BbeI 1514 GGCGCC GGCGC^C
BclI 5577 TGATCA T^GATCA
BglII 6695 AGATCT A^GATCT
BlnI 1147 CCTAGG C^CTAGG
BlpI 2567 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2567 GCTGAGC GC^T[ACGT]AGC
BsaBI 3658 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 3048 TGTACA T^GTACA
BspLU11I 2397 ACATGT A^CATGT
BsrBI 40 GAGCGG CCGCTC(-3/-3)
BsrGI 3048 TGTACA T^GTACA
CelII 2567 GCTGAGC GC^T[ACGT]AGC
CpoI 3854 CGGACCG CG^G[AT]CCG
DraI 5963 TTTAAA TTT^AAA
Eam1105I 6644 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3473 AGCGCT AGC^GCT
EcoT22I 5700 ATGCAT ATGCA^T
FbaI 5577 TGATCA T^GATCA
HpaI 233 GTTAAC GTT^AAC
KasI 1514 GGCGCC G^GCGCC
MamI 3658 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 4954 GCCGGC GCC^GGC
NarI 1514 GGCGCC GG^CGCC
NgoMIV 4954 GCCGGC G^CCGGC
NsiI 5700 ATGCAT ATGCA^T
PflFI 139 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 4000 ATTAAT AT^TAAT
RsrII 3854 CGGACCG CG^G[AT]CCG
SapI 5545 GCTCTTC GCTCTTC(1/4)
ScaI 5025 AGTACT AGT^ACT
SfiI 4279 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1514 GGCGCC GGC^GCC
SnaBI 661 TACGTA TAC^GTA
SphI 1089 GCATGC GCATG^C
SspBI 3048 TGTACA T^GTACA
SspI 3997 AATATT AAT^ATT
Tth111I 139 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AatII 854 GACGTC GACGT^C
3758 GACGTC
AcsI 1452 AAATTC [GA]^AATT[CT]
2135 AAATTC
ApoI 1452 AAATTC [GA]^AATT[CT]
2135 AAATTC
BbrPI 1746 CACGTG CAC^GTG
6514 CACGTG
BsmI 5300 GCATTC GAATGC(1/-1)
5842 GCATTC
BspHI 2973 TCATGA T^CATGA
5574 TCATGA
DrdI 1844 GACTTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6283 GACATGAGTGTC
HaeII 1514 GGCGCC [GA]GCGC^[CT]
3473 AGCGCT
HincII 233 GTTAAC GT[CT]^[GA]AC
3062 GTTGAC
HindII 233 GTTAAC GT[CT]^[GA]AC
3062 GTTGAC
KspI 3244 CCGCGG CCGC^GG
5367 CCGCGG
PaeR7I 2123 CTCGAG C^TCGAG
5640 CTCGAG
PmaCI 1746 CACGTG CAC^GTG
6514 CACGTG
PmlI 1746 CACGTG CAC^GTG
6514 CACGTG
RcaI 2973 TCATGA T^CATGA
5574 TCATGA
SacII 3244 CCGCGG CCGC^GG
5367 CCGCGG
SexAI 1581 ACCTGGT A^CC[AT]GGT
5526 ACCAGGT
XhoI 2123 CTCGAG C^TCGAG
5640 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI Asp700I AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvCI BbvI BcgI
BciVI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssHII BssKI BssSI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PshAI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI XmnI