##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AflII AflIII AgeI
Alw44I ApaI ApaLI AscI Asp700I
Asp718I AspI AvrII BamHI BbrPI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsiWI BspDI BspLU11I BsrBI
BssHII Bst1107I BstBI BstZ17I CelII
ClaI CpoI EcoRI EcoRV FseI
KpnI MamI MluI NaeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PvuI RsrII SalI
SfiI SfuI SgrAI SnaBI SpeI
Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 2351 GTCTAC GT^[AC][GT]AC
AccIII 1472 TCCGGA T^CCGGA
AclI 726 AACGTT AA^CGTT
AhdI 3453 GACCAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 5254 AGCGCT AGC^GCT
AspEI 3453 GACCAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1247 TGCGCA TGC^GCA
BcgI 653 CGACAAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI 1958 GGTCTC GGTCTC(1/5)
BsaWI 1472 TCCGGA [AT]^CCGG[AT]
BseAI 1472 TCCGGA T^CCGGA
BsiEI 2179 CGGCCG CG[GA][CT]^CG
Bsp1407I 4471 TGTACA T^GTACA
BspEI 1472 TCCGGA T^CCGGA
BsrGI 4471 TGTACA T^GTACA
BssSI 2795 CACGAG CACGAG(-5/-1)
BstAPI 588 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2709 GGTGACC G^GT[ACGT]ACC
BstPI 2709 GGTGACC G^GT[ACGT]ACC
BstXI 4658 CCAGCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 5273 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3323 GACGCTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2179 CGGCCG C^GGCCG
Eam1105I 3453 GACCAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2179 CGGCCG C^GGCCG
Eco47III 5254 AGCGCT AGC^GCT
Eco52I 2179 CGGCCG C^GGCCG
EcoO65I 2709 GGTGACC G^GT[ACGT]ACC
FspI 1247 TGCGCA TGC^GCA
KspI 94 CCGCGG CCGC^GG
MroI 1472 TCCGGA T^CCGGA
NdeI 1434 CATATG CA^TATG
Psp1406I 726 AACGTT AA^CGTT
SacII 94 CCGCGG CCGC^GG
SbfI 1848 CCTGCAGG CCTGCA^GG
ScaI 1298 AGTACT AGT^ACT
SmaI 10 CCCGGG CCC^GGG
Sse8387I 1848 CCTGCAGG CCTGCA^GG
SspBI 4471 TGTACA T^GTACA
SwaI 5038 ATTTAAAT ATTT^AAAT
XbaI 5312 TCTAGA T^CTAGA
XmaCI 10 CCCGGG C^CCGGG
XmaI 10 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AseI 2663 ATTAAT AT^TAAT
4011 ATTAAT
AsnI 2663 ATTAAT AT^TAAT
4011 ATTAAT
AvaI 10 CCCGGG C^[CT]CG[GA]G
4608 CCCGAG
BalI 2431 TGGCCA TGG^CCA
3311 TGGCCA
BbeI 2805 GGCGCC GGCGC^C
3710 GGCGCC
BbsI 244 GAAGAC GAAGAC(2/6)
5285 GAAGAC
BbvCI 1940 CCTCAGC CCTCAGC(-5/-2)
3066 CCTCAGC
BclI 2270 TGATCA T^GATCA
5504 TGATCA
BglII 141 AGATCT A^GATCT
4976 AGATCT
BpuAI 244 GAAGAC GAAGAC(2/6)
5285 GAAGAC
BsmBI 1165 CGTCTC CGTCTC(1/5)
54 GAGACG
BsoBI 10 CCCGGG C^[CT]CG[GA]G
4608 CCCGAG
EcoT22I 3733 ATGCAT ATGCA^T
4077 ATGCAT
FbaI 2270 TGATCA T^GATCA
5504 TGATCA
HindIII 1856 AAGCTT A^AGCTT
2057 AAGCTT
HpaI 2438 GTTAAC GTT^AAC
5388 GTTAAC
KasI 2805 GGCGCC G^GCGCC
3710 GGCGCC
MfeI 917 CAATTG C^AATTG
5672 CAATTG
MluNI 2431 TGGCCA TGG^CCA
3311 TGGCCA
MscI 2431 TGGCCA TGG^CCA
3311 TGGCCA
MunI 917 CAATTG C^AATTG
5672 CAATTG
NarI 2805 GGCGCC GG^CGCC
3710 GGCGCC
NsiI 3733 ATGCAT ATGCA^T
4077 ATGCAT
PflMI 4440 CCACACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5093 CCAGACACTGG
PshBI 2663 ATTAAT AT^TAAT
4011 ATTAAT
SacI 46 GAGCTC GAGCT^C
3553 GAGCTC
SapI 777 GAAGAGC GCTCTTC(1/4)
1376 GAAGAGC
SexAI 1216 ACCTGGT A^CC[AT]GGT
5626 ACCAGGT
SfoI 2805 GGCGCC GGC^GCC
3710 GGCGCC
SphI 1627 GCATGC GCATG^C
1678 GCATGC
SspI 4708 AATATT AAT^ATT
5401 AATATT
StuI 3984 AGGCCT AGG^CCT
4844 AGGCCT
Van91I 4440 CCACACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5093 CCAGACACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AvaII BanI BanII BbvI
BciVI BcnI BfaI BglI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspHI BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII