##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AflII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI AspEI
AvrII BbeI BbrPI BcgI BciVI
BfrI BlnI BsaAI BsiWI BspDI
BspHI BsrFI BssHII BssSI Bst1107I
BstEII BstPI BstZ17I Cfr10I ClaI
DraII DrdI Eam1105I Eco47III EcoO109I
EcoO65I FseI HindIII KasI NaeI
NarI NdeI NgoMIV NheI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
RcaI SalI SbfI SexAI SfoI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 2665 GGTACC G^GTACC
AccIII 494 TCCGGA T^CCGGA
AclI 1085 AACGTT AA^CGTT
AcyI 36 GGCGTC G[GA]^CG[CT]C
Alw44I 1337 GTGCAC G^TGCAC
ApaLI 1337 GTGCAC G^TGCAC
Asp700I 1157 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2665 GGTACC G^GTACC
AviII 13 TGCGCA TGC^GCA
BalI 679 TGGCCA TGG^CCA
BamHI 4222 GGATCC G^GATCC
BclI 3864 TGATCA T^GATCA
BsaHI 36 GGCGTC G[GA]^CG[CT]C
BsaI 167 GAGACC GGTCTC(1/5)
BseAI 494 TCCGGA T^CCGGA
Bsp1407I 5653 TGTACA T^GTACA
BspEI 494 TCCGGA T^CCGGA
BsrDI 1055 CATTGC GCAATG(2/0)
BsrGI 5653 TGTACA T^GTACA
BstBI 3003 TTCGAA TT^CGAA
CpoI 209 CGGACCG CG^G[AT]CCG
DraIII 319 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 1483 GATATC GAT^ATC
EcoT22I 2352 ATGCAT ATGCA^T
FbaI 3864 TGATCA T^GATCA
FspI 13 TGCGCA TGC^GCA
HaeII 6 GGCGCT [GA]GCGC^[CT]
Hin1I 36 GGCGTC G[GA]^CG[CT]C
HpaI 822 GTTAAC GTT^AAC
KpnI 2665 GGTACC GGTAC^C
KspI 123 CCGCGG CCGC^GG
MfeI 917 CAATTG C^AATTG
MluI 5110 ACGCGT A^CGCGT
MluNI 679 TGGCCA TGG^CCA
MroI 494 TCCGGA T^CCGGA
MscI 679 TGGCCA TGG^CCA
MunI 917 CAATTG C^AATTG
NotI 233 GCGGCCGC GC^GGCCGC
NsiI 2352 ATGCAT ATGCA^T
NspV 3003 TTCGAA TT^CGAA
Psp1406I 1085 AACGTT AA^CGTT
RsrII 209 CGGACCG CG^G[AT]CCG
SacII 123 CCGCGG CCGC^GG
SapI 3694 GAAGAGC GCTCTTC(1/4)
SfiI 226 GGCCTCTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 3003 TTCGAA TT^CGAA
SspBI 5653 TGTACA T^GTACA
XcmI 3977 CCAATCCACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1157 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 2321 GTCTAC GT^[AC][GT]AC
5073 GTCTAC
AocI 2669 CCTTAGG CC^T[ACGT]AGG
4849 CCTAAGG
BlpI 1324 GCTAAGC GC^T[ACGT]AGC
2200 GCTCAGC
Bpu1102I 1324 GCTAAGC GC^T[ACGT]AGC
2200 GCTCAGC
BsaBI 1479 GATAGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4346 GATCTATATC
BsaWI 212 ACCGGA [AT]^CCGG[AT]
494 TCCGGA
BsmBI 1958 CGTCTC CGTCTC(1/5)
1794 GAGACG
BsrBI 276 CCGCTC CCGCTC(-3/-3)
422 GAGCGG
BstXI 1060 CCACCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2927 CCAAATGTTTGG
Bsu36I 2669 CCTTAGG CC^T[ACGT]AGG
4849 CCTAAGG
CelII 1324 GCTAAGC GC^T[ACGT]AGC
2200 GCTCAGC
EagI 205 CGGCCG C^GGCCG
234 CGGCCG
EclXI 205 CGGCCG C^GGCCG
234 CGGCCG
Eco52I 205 CGGCCG C^GGCCG
234 CGGCCG
Eco81I 2669 CCTTAGG CC^T[ACGT]AGG
4849 CCTAAGG
EcoNI 939 CCTTGTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2833 CCTTCTGGAGG
HincII 822 GTTAAC GT[CT]^[GA]AC
3707 GTCAAC
HindII 822 GTTAAC GT[CT]^[GA]AC
3707 GTCAAC
MaeII 1086 ACGT A^CGT
3578 ACGT
MamI 1479 GATAGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4346 GATCTATATC
NcoI 446 CCATGG C^CATGG
5772 CCATGG
SacI 723 GAGCTC GAGCT^C
3842 GAGCTC
SmlI 1961 CTCAAG C^T[CT][GA]AG
4085 CTCAAG
SpeI 793 ACTAGT A^CTAGT
3850 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AspI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspLU11I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XhoII