##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
Alw44I ApaLI AscI Asp718I AspEI
AviII BamHI BbrPI BfrI BsaAI
BsaBI BseAI BsiWI BspEI BspHI
BstBI BstEII BstPI Eam1105I EcoO65I
EcoRV FseI FspI HindIII HpaI
KpnI MamI MfeI MluI MroI
MunI NdeI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PvuI RcaI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AclI 6098 AACGTT AA^CGTT
AflIII 4976 ACATGT A^C[GA][CT]GT
AseI 1956 ATTAAT AT^TAAT
AsnI 1956 ATTAAT AT^TAAT
AspI 3797 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4738 CCTAGG C^CTAGG
BbeI 437 GGCGCC GGCGC^C
BcgI 4420 GCAGCTCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5222 GGATAC GTATCC(6/5)
BclI 5556 TGATCA T^GATCA
BglI 2468 GCCCCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1049 AGATCT A^GATCT
BlnI 4738 CCTAGG C^CTAGG
BsmI 2190 GAATGC GAATGC(1/-1)
BspLU11I 4976 ACATGT A^CATGT
BssSI 48 CTCGTG CACGAG(-5/-1)
Bst1107I 5574 GTATAC GTA^TAC
BstZ17I 5574 GTATAC GTA^TAC
CpoI 5611 CGGACCG CG^G[AT]CCG
DraI 5020 TTTAAA TTT^AAA
DrdI 3758 GACCTCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 423 CGGCCG C^GGCCG
EclXI 423 CGGCCG C^GGCCG
Eco52I 423 CGGCCG C^GGCCG
EcoT22I 4885 ATGCAT ATGCA^T
FbaI 5556 TGATCA T^GATCA
KasI 437 GGCGCC G^GCGCC
NarI 437 GGCGCC GG^CGCC
NsiI 4885 ATGCAT ATGCA^T
NspI 4976 ACATGT [GA]CATG^[CT]
PaeR7I 2702 CTCGAG C^TCGAG
PflFI 3797 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 5707 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1956 ATTAAT AT^TAAT
Psp1406I 6098 AACGTT AA^CGTT
RsrII 5611 CGGACCG CG^G[AT]CCG
SalI 5376 GTCGAC G^TCGAC
SfoI 437 GGCGCC GGC^GCC
SspI 1507 AATATT AAT^ATT
Tth111I 3797 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2702 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 3376 CCTCAGG CC^T[ACGT]AGG
6089 CCTCAGG
BbvCI 5585 CCTCAGC CCTCAGC(-5/-2)
1917 GCTGAGG
BsaWI 484 ACCGGA [AT]^CCGG[AT]
2260 ACCGGA
BsmBI 3160 CGTCTC CGTCTC(1/5)
5006 GAGACG
Bsp1407I 2925 TGTACA T^GTACA
4265 TGTACA
BspDI 1170 ATCGAT AT^CGAT
1209 ATCGAT
BsrDI 2654 CATTGC GCAATG(2/0)
6029 CATTGC
BsrGI 2925 TGTACA T^GTACA
4265 TGTACA
BssHII 2 GCGCGC G^CGCGC
571 GCGCGC
Bsu36I 3376 CCTCAGG CC^T[ACGT]AGG
6089 CCTCAGG
ClaI 1170 ATCGAT AT^CGAT
1209 ATCGAT
DraIII 3296 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
5521 CACAAGGTG
Eco81I 3376 CCTCAGG CC^T[ACGT]AGG
6089 CCTCAGG
EcoNI 2701 CCTCGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3098 CCTCAATAAGG
HgaI 735 GCGTC GACGC(5/10)
2037 GCGTC
KspI 66 CCGCGG CCGC^GG
712 CCGCGG
NheI 673 GCTAGC G^CTAGC
2979 GCTAGC
SacII 66 CCGCGG CCGC^GG
712 CCGCGG
SapI 5398 GAAGAGC GCTCTTC(1/4)
3544 GCTCTTC
SspBI 2925 TGTACA T^GTACA
4265 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AatII AccI AccII AciI AcsI
AcyI AfaI AluI AlwI AlwNI
Aor51HI ApaI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco47III
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI XmnI