##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AgeI AhdI ApaI AscI
Asp718I AspEI AspI AviII AvrII
BbrPI BbvCI BglI BlnI BsaAI
BsiEI BsiWI BspDI BspLU11I BsrBI
BstBI ClaI CpoI DrdI EagI
Eam1105I EclXI Eco52I EcoNI EcoRV
FseI FspI HpaI KpnI KspI
MfeI MluI MunI NdeI NotI
NruI NspV PaeR7I PflFI PinAI
PmaCI PmlI PpuMI PshAI PvuI
RsrII SacII SalI SbfI SfiI
SfuI SgrAI SnaBI SphI Sse8387I
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 87 GACGTC GACGT^C
AccIII 4029 TCCGGA T^CCGGA
AflIII 4248 ACACGT A^C[GA][CT]GT
Alw44I 3349 GTGCAC G^TGCAC
Aor51HI 4938 AGCGCT AGC^GCT
ApaLI 3349 GTGCAC G^TGCAC
BbeI 4265 GGCGCC GGCGC^C
BciVI 6901 GTATCC GTATCC(6/5)
BsaWI 4029 TCCGGA [AT]^CCGG[AT]
BseAI 4029 TCCGGA T^CCGGA
Bsp1407I 6876 TGTACA T^GTACA
BspEI 4029 TCCGGA T^CCGGA
BspHI 1598 TCATGA T^CATGA
BspMI 5351 ACCTGC ACCTGC(4/8)
BsrFI 5947 GCCGGC [GA]^CCGG[CT]
BsrGI 6876 TGTACA T^GTACA
BssSI 499 CACGAG CACGAG(-5/-1)
Bst1107I 5097 GTATAC GTA^TAC
BstEII 5244 GGTGACC G^GT[ACGT]ACC
BstPI 5244 GGTGACC G^GT[ACGT]ACC
BstXI 5324 CCAGCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 5097 GTATAC GTA^TAC
Cfr10I 5947 GCCGGC [GA]^CCGG[CT]
Eco47III 4938 AGCGCT AGC^GCT
EcoO65I 5244 GGTGACC G^GT[ACGT]ACC
EcoT22I 6225 ATGCAT ATGCA^T
KasI 4265 GGCGCC G^GCGCC
MroI 4029 TCCGGA T^CCGGA
NaeI 5947 GCCGGC GCC^GGC
NarI 4265 GGCGCC GG^CGCC
NgoMIV 5947 GCCGGC G^CCGGC
NsiI 6225 ATGCAT ATGCA^T
NspI 1130 ACATGC [GA]CATG^[CT]
PacI 1831 TTAATTAA TTAAT^TAA
PmeI 6769 GTTTAAAC GTTT^AAAC
RcaI 1598 TCATGA T^CATGA
SexAI 5984 ACCAGGT A^CC[AT]GGT
SfoI 4265 GGCGCC GGC^GCC
SmaI 702 CCCGGG CCC^GGG
SpeI 5461 ACTAGT A^CTAGT
SspBI 6876 TGTACA T^GTACA
StuI 4716 AGGCCT AGG^CCT
XmaCI 702 CCCGGG C^CCGGG
XmaI 702 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 5097 GTATAC GT^[AC][GT]AC
5347 GTCTAC
AclI 2190 AACGTT AA^CGTT
4195 AACGTT
AflII 403 CTTAAG C^TTAAG
1810 CTTAAG
AocI 181 CCTAAGG CC^T[ACGT]AGG
6316 CCTAAGG
AvaI 702 CCCGGG C^[CT]CG[GA]G
5540 CCCGAG
BamHI 2369 GGATCC G^GATCC
3315 GGATCC
BcgI 4386 CGAAAAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
6232 GCACAGTTTTCG
BfrI 403 CTTAAG C^TTAAG
1810 CTTAAG
BlpI 710 GCTCAGC GC^T[ACGT]AGC
1536 GCTCAGC
Bpu1102I 710 GCTCAGC GC^T[ACGT]AGC
1536 GCTCAGC
BsaBI 830 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4473 GATGAATATC
BsaI 4738 GAGACC GGTCTC(1/5)
5334 GGTCTC
BsmI 3377 GAATGC GAATGC(1/-1)
5426 GCATTC
BsoBI 702 CCCGGG C^[CT]CG[GA]G
5540 CCCGAG
BstAPI 1125 GCATTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3630 GCAAAAAATGC
Bsu36I 181 CCTAAGG CC^T[ACGT]AGG
6316 CCTAAGG
CelII 710 GCTCAGC GC^T[ACGT]AGC
1536 GCTCAGC
Eco81I 181 CCTAAGG CC^T[ACGT]AGG
6316 CCTAAGG
EcoRI 921 GAATTC G^AATTC
1568 GAATTC
HgaI 6842 GACGC GACGC(5/10)
5479 GCGTC
HincII 3157 GTCAAC GT[CT]^[GA]AC
5883 GTTGAC
HindII 3157 GTCAAC GT[CT]^[GA]AC
5883 GTTGAC
MamI 830 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4473 GATGAATATC
NheI 300 GCTAGC G^CTAGC
1273 GCTAGC
PflMI 1263 CCATTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3886 CCAGATGGTGG
Psp1406I 2190 AACGTT AA^CGTT
4195 AACGTT
SacI 2404 GAGCTC GAGCT^C
5659 GAGCTC
SapI 2401 GAAGAGC GCTCTTC(1/4)
5656 GAAGAGC
ScaI 3144 AGTACT AGT^ACT
4487 AGTACT
TaqI 200 TCGA T^CGA
6324 TCGA
TthHB8I 200 TCGA T^CGA
6324 TCGA
Van91I 1263 CCATTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3886 CCAGATGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaII BalI
BanI BanII BbsI BbvI BclI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BsrDI BsrI BssHII
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XbaI XcmI XhoII XmnI