##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccIII AflII AgeI AhdI
Aor51HI AscI AspEI AspI AviII
AvrII BamHI BbeI BbrPI BclI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaWI BseAI BsiWI Bsp1407I BspEI
BsrGI BssHII BstBI BstEII BstPI
CelII CpoI DraIII DrdI Eam1105I
Eco47III EcoNI EcoO65I FbaI FseI
FspI KasI MfeI MluI MroI
MunI NarI NheI NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PvuI RsrII
SacI SalI SexAI SfiI SfoI
SfuI SgrAI SnaBI SphI SspBI
SspI StuI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 887 GGTACC G^GTACC
AccI 1640 GTATAC GT^[AC][GT]AC
AclI 3610 AACGTT AA^CGTT
AcyI 257 GGCGTC G[GA]^CG[CT]C
AflIII 1960 ACATGT A^C[GA][CT]GT
Alw44I 29 GTGCAC G^TGCAC
ApaI 4648 GGGCCC GGGCC^C
ApaLI 29 GTGCAC G^TGCAC
AseI 4004 ATTAAT AT^TAAT
AsnI 4004 ATTAAT AT^TAAT
Asp718I 887 GGTACC G^GTACC
BcgI 75 CGAGGACCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 2665 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 257 GGCGTC G[GA]^CG[CT]C
BsaI 994 GAGACC GGTCTC(1/5)
BsmBI 4397 GAGACG CGTCTC(1/5)
BspDI 2028 ATCGAT AT^CGAT
BspLU11I 1960 ACATGT A^CATGT
BsrBI 3930 GAGCGG CCGCTC(-3/-3)
BssSI 4047 CACGAG CACGAG(-5/-1)
Bst1107I 1640 GTATAC GTA^TAC
BstAPI 4371 GCAAAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 4719 CCAGAATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1640 GTATAC GTA^TAC
ClaI 2028 ATCGAT AT^CGAT
DraI 1001 TTTAAA TTT^AAA
EcoRV 2892 GATATC GAT^ATC
EcoT22I 4428 ATGCAT ATGCA^T
HaeII 70 AGCGCC [GA]GCGC^[CT]
Hin1I 257 GGCGTC G[GA]^CG[CT]C
HindIII 3545 AAGCTT A^AGCTT
KpnI 887 GGTACC GGTAC^C
KspI 41 CCGCGG CCGC^GG
MamI 2665 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 4428 ATGCAT ATGCA^T
PaeR7I 1618 CTCGAG C^TCGAG
PflMI 4536 CCACCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 143 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4004 ATTAAT AT^TAAT
Psp1406I 3610 AACGTT AA^CGTT
SacII 41 CCGCGG CCGC^GG
SmaI 282 CCCGGG CCC^GGG
Van91I 4536 CCACCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2436 TCTAGA T^CTAGA
XhoI 1618 CTCGAG C^TCGAG
XmaCI 282 CCCGGG C^CCGGG
XmaI 282 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 334 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
2491 CAGCTACTG
AocI 2295 CCTCAGG CC^T[ACGT]AGG
3947 CCTCAGG
BalI 605 TGGCCA TGG^CCA
3393 TGGCCA
BanII 4042 GAGCCC G[GA]GC[CT]^C
4648 GGGCCC
BbvCI 2856 CCTCAGC CCTCAGC(-5/-2)
1494 GCTGAGG
BsrFI 239 GCCGGC [GA]^CCGG[CT]
4107 GCCGGC
Bsu36I 2295 CCTCAGG CC^T[ACGT]AGG
3947 CCTCAGG
Cfr10I 239 GCCGGC [GA]^CCGG[CT]
4107 GCCGGC
Eco81I 2295 CCTCAGG CC^T[ACGT]AGG
3947 CCTCAGG
EcoRI 57 GAATTC G^AATTC
4608 GAATTC
HgaI 258 GCGTC GACGC(5/10)
4350 GCGTC
HpaI 4076 GTTAAC GTT^AAC
4241 GTTAAC
MluNI 605 TGGCCA TGG^CCA
3393 TGGCCA
MscI 605 TGGCCA TGG^CCA
3393 TGGCCA
NaeI 239 GCCGGC GCC^GGC
4107 GCCGGC
NgoMIV 239 GCCGGC G^CCGGC
4107 GCCGGC
SbfI 2453 CCTGCAGG CCTGCA^GG
4227 CCTGCAGG
ScaI 1199 AGTACT AGT^ACT
1803 AGTACT
SpeI 2400 ACTAGT A^CTAGT
3564 ACTAGT
Sse8387I 2453 CCTGCAGG CCTGCA^GG
4227 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaI
AvaII BanI BbsI BbvI BciVI
BcnI BfaI BglI BglII BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RcaI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI