##################################
# NO CUT ENZYMES (   74 enzymes) #
##################################

AatII       AccIII      AflII       AgeI        AhdI        
Aor51HI     AscI        AspEI       AspI        AviII       
AvrII       BamHI       BbeI        BbrPI       BclI        
BfrI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaWI       BseAI       BsiWI       Bsp1407I    BspEI       
BsrGI       BssHII      BstBI       BstEII      BstPI       
CelII       CpoI        DraIII      DrdI        Eam1105I    
Eco47III    EcoNI       EcoO65I     FbaI        FseI        
FspI        KasI        MfeI        MluI        MroI        
MunI        NarI        NheI        NotI        NruI        
NspV        PacI        PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PvuI        RsrII       
SacI        SalI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SphI        SspBI       
SspI        StuI        SwaI        Tth111I     


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I        887  GGTACC               G^GTACC
AccI         1640  GTATAC               GT^[AC][GT]AC
AclI         3610  AACGTT               AA^CGTT
AcyI          257  GGCGTC               G[GA]^CG[CT]C
AflIII       1960  ACATGT               A^C[GA][CT]GT
Alw44I         29  GTGCAC               G^TGCAC
ApaI         4648  GGGCCC               GGGCC^C
ApaLI          29  GTGCAC               G^TGCAC
AseI         4004  ATTAAT               AT^TAAT
AsnI         4004  ATTAAT               AT^TAAT
Asp718I       887  GGTACC               G^GTACC
BcgI           75  CGAGGACCGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI        2665  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         257  GGCGTC               G[GA]^CG[CT]C
BsaI          994  GAGACC               GGTCTC(1/5)
BsmBI        4397  GAGACG               CGTCTC(1/5)
BspDI        2028  ATCGAT               AT^CGAT
BspLU11I     1960  ACATGT               A^CATGT
BsrBI        3930  GAGCGG               CCGCTC(-3/-3)
BssSI        4047  CACGAG               CACGAG(-5/-1)
Bst1107I     1640  GTATAC               GTA^TAC
BstAPI       4371  GCAAAAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        4719  CCAGAATACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1640  GTATAC               GTA^TAC
ClaI         2028  ATCGAT               AT^CGAT
DraI         1001  TTTAAA               TTT^AAA
EcoRV        2892  GATATC               GAT^ATC
EcoT22I      4428  ATGCAT               ATGCA^T
HaeII          70  AGCGCC               [GA]GCGC^[CT]
Hin1I         257  GGCGTC               G[GA]^CG[CT]C
HindIII      3545  AAGCTT               A^AGCTT
KpnI          887  GGTACC               GGTAC^C
KspI           41  CCGCGG               CCGC^GG
MamI         2665  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI         4428  ATGCAT               ATGCA^T
PaeR7I       1618  CTCGAG               C^TCGAG
PflMI        4536  CCACCATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         143  GACCCCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        4004  ATTAAT               AT^TAAT
Psp1406I     3610  AACGTT               AA^CGTT
SacII          41  CCGCGG               CCGC^GG
SmaI          282  CCCGGG               CCC^GGG
Van91I       4536  CCACCATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         2436  TCTAGA               T^CTAGA
XhoI         1618  CTCGAG               C^TCGAG
XmaCI         282  CCCGGG               C^CCGGG
XmaI          282  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwNI         334  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2491  CAGCTACTG           
AocI         2295  CCTCAGG              CC^T[ACGT]AGG
             3947  CCTCAGG             
BalI          605  TGGCCA               TGG^CCA
             3393  TGGCCA              
BanII        4042  GAGCCC               G[GA]GC[CT]^C
             4648  GGGCCC              
BbvCI        2856  CCTCAGC              CCTCAGC(-5/-2)
             1494  GCTGAGG             
BsrFI         239  GCCGGC               [GA]^CCGG[CT]
             4107  GCCGGC              
Bsu36I       2295  CCTCAGG              CC^T[ACGT]AGG
             3947  CCTCAGG             
Cfr10I        239  GCCGGC               [GA]^CCGG[CT]
             4107  GCCGGC              
Eco81I       2295  CCTCAGG              CC^T[ACGT]AGG
             3947  CCTCAGG             
EcoRI          57  GAATTC               G^AATTC
             4608  GAATTC              
HgaI          258  GCGTC                GACGC(5/10)
             4350  GCGTC               
HpaI         4076  GTTAAC               GTT^AAC
             4241  GTTAAC              
MluNI         605  TGGCCA               TGG^CCA
             3393  TGGCCA              
MscI          605  TGGCCA               TGG^CCA
             3393  TGGCCA              
NaeI          239  GCCGGC               GCC^GGC
             4107  GCCGGC              
NgoMIV        239  GCCGGC               G^CCGGC
             4107  GCCGGC              
SbfI         2453  CCTGCAGG             CCTGCA^GG
             4227  CCTGCAGG            
ScaI         1199  AGTACT               AGT^ACT
             1803  AGTACT              
SpeI         2400  ACTAGT               A^CTAGT
             3564  ACTAGT              
Sse8387I     2453  CCTGCAGG             CCTGCA^GG
             4227  CCTGCAGG            


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        Asp700I     AspHI       AvaI        
AvaII       BanI        BbsI        BbvI        BciVI       
BcnI        BfaI        BglI        BglII       BmyI        
BpmI        BpuAI       BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspHI       
BspMI       BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EagI        EarI        EclXI       Eco52I      
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeI        NdeII       
NlaIII      NlaIV       NspI        PleI        PstI        
PvuII       RcaI        RsaI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII       XmnI