##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AscI AseI AsnI Asp700I Asp718I
AvrII BfrI BlnI BsaBI BsgI
BsiWI BsmBI BspDI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
EcoT22I FseI HgaI KpnI KspI
MamI MfeI MluI MunI NaeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshBI
Psp1406I RsrII SacI SacII SalI
SfuI SgrAI SnaBI SpeI SphI
StuI SwaI XbaI XcmI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 628 TCCGGA T^CCGGA
AspI 1458 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4084 TGCGCA TGC^GCA
BciVI 3596 GTATCC GTATCC(6/5)
BfaI 798 CTAG C^TAG
BseAI 628 TCCGGA T^CCGGA
BspEI 628 TCCGGA T^CCGGA
BspHI 2398 TCATGA T^CATGA
BspLU11I 298 ACATGT A^CATGT
BspMI 275 GCAGGT ACCTGC(4/8)
BsrBI 3360 CCGCTC CCGCTC(-3/-3)
BsrFI 2798 GCCGGT [GA]^CCGG[CT]
Cfr10I 2798 GCCGGT [GA]^CCGG[CT]
DraIII 1798 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3859 GACTCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2087 CGGCCG C^GGCCG
EclXI 2087 CGGCCG C^GGCCG
Eco52I 2087 CGGCCG C^GGCCG
EcoNI 1525 CCTTGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1315 GATATC GAT^ATC
FspI 4084 TGCGCA TGC^GCA
HincII 62 GTTAAC GT[CT]^[GA]AC
HindII 62 GTTAAC GT[CT]^[GA]AC
HpaI 62 GTTAAC GTT^AAC
MaeI 798 CTAG C^TAG
MroI 628 TCCGGA T^CCGGA
NdeI 2984 CATATG CA^TATG
PaeR7I 3023 CTCGAG C^TCGAG
PflFI 1458 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 19 CCAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 1042 CGATCG CGAT^CG
RcaI 2398 TCATGA T^CATGA
SbfI 272 CCTGCAGG CCTGCA^GG
SexAI 1459 ACCTGGT A^CC[AT]GGT
SfiI 1655 GGCCATTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 272 CCTGCAGG CCTGCA^GG
SspI 4490 AATATT AAT^ATT
Tth111I 1458 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 19 CCAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3023 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 44 enzymes) #
###################################
AccII 2103 CGCG CG^CG
3416 CGCG
AcyI 1401 GGCGCC G[GA]^CG[CT]C
2569 GGCGCC
AhdI 74 GACCTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4045 GACTTCCCGTC
AocI 858 CCTGAGG CC^T[ACGT]AGG
1714 CCTCAGG
AspEI 74 GACCTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4045 GACTTCCCGTC
BalI 1654 TGGCCA TGG^CCA
3531 TGGCCA
BamHI 168 GGATCC G^GATCC
420 GGATCC
BbeI 1401 GGCGCC GGCGC^C
2569 GGCGCC
BbvCI 339 CCTCAGC CCTCAGC(-5/-2)
2378 CCTCAGC
BclI 968 TGATCA T^GATCA
1958 TGATCA
BglII 1033 AGATCT A^GATCT
3253 AGATCT
BlpI 1136 GCTGAGC GC^T[ACGT]AGC
3075 GCTGAGC
Bpu1102I 1136 GCTGAGC GC^T[ACGT]AGC
3075 GCTGAGC
BsaHI 1401 GGCGCC G[GA]^CG[CT]C
2569 GGCGCC
BsaWI 628 TCCGGA [AT]^CCGG[AT]
2524 ACCGGA
BsiEI 1042 CGATCG CG[GA][CT]^CG
2087 CGGCCG
BsmI 782 GAATGC GAATGC(1/-1)
4274 GCATTC
Bsp1407I 3421 TGTACA T^GTACA
4283 TGTACA
BsrGI 3421 TGTACA T^GTACA
4283 TGTACA
BssSI 964 CTCGTG CACGAG(-5/-1)
2415 CTCGTG
BstAPI 1862 GCACGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2623 GCACCAGCTGC
BstUI 2103 CGCG CG^CG
3416 CGCG
Bsu36I 858 CCTGAGG CC^T[ACGT]AGG
1714 CCTCAGG
CelII 1136 GCTGAGC GC^T[ACGT]AGC
3075 GCTGAGC
Eam1105I 74 GACCTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4045 GACTTCCCGTC
Eco81I 858 CCTGAGG CC^T[ACGT]AGG
1714 CCTCAGG
FbaI 968 TGATCA T^GATCA
1958 TGATCA
Hin1I 1401 GGCGCC G[GA]^CG[CT]C
2569 GGCGCC
HindIII 4187 AAGCTT A^AGCTT
4429 AAGCTT
KasI 1401 GGCGCC G^GCGCC
2569 GGCGCC
MluNI 1654 TGGCCA TGG^CCA
3531 TGGCCA
MscI 1654 TGGCCA TGG^CCA
3531 TGGCCA
MvnI 2103 CGCG CG^CG
3416 CGCG
NarI 1401 GGCGCC GG^CGCC
2569 GGCGCC
NcoI 40 CCATGG C^CATGG
2834 CCATGG
PshAI 2144 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2486 GACACAGGTC
PstI 273 CTGCAG CTGCA^G
2630 CTGCAG
SapI 2015 GAAGAGC GCTCTTC(1/4)
2618 GAAGAGC
ScaI 613 AGTACT AGT^ACT
1889 AGTACT
SfoI 1401 GGCGCC GGC^GCC
2569 GGCGCC
SmaI 2431 CCCGGG CCC^GGG
3479 CCCGGG
SspBI 3421 TGTACA T^GTACA
4283 TGTACA
XmaCI 2431 CCCGGG C^CCGGG
3479 CCCGGG
XmaI 2431 CCCGGG C^CCGGG
3479 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI Aor51HI
ApaI ApaLI ApoI AspHI AvaI
AvaII BanI BanII BbrPI BbsI
BbvI BcgI BcnI BglI BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrDI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco47III EcoO109I EcoO65I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PmaCI PmlI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII