##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AviII       BbeI        BbrPI       BcgI        BclI        
BfrI        BsaAI       BsaBI       BseAI       BsiEI       
BsiWI       BspEI       BssHII      BstBI       CpoI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      FbaI        FseI        FspI        HaeII       
HpaI        KasI        KpnI        KspI        MamI        
MluI        MroI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        SfiI        SfoI        SfuI        SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
SwaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI         5415  GTATAC               GT^[AC][GT]AC
AccII          64  CGCG                 CG^CG
AcyI         4212  GACGCC               G[GA]^CG[CT]C
AgeI         5218  ACCGGT               A^CCGGT
Asp700I      5532  GAAGTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         3192  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII          10  CCTAGG               C^CTAGG
BamHI        3252  GGATCC               G^GATCC
BanI         2879  GGCACC               G^G[CT][GA]CC
BlnI           10  CCTAGG               C^CTAGG
BlpI         2721  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     2721  GCTAAGC              GC^T[ACGT]AGC
BsaHI        4212  GACGCC               G[GA]^CG[CT]C
BsaWI        5218  ACCGGT               [AT]^CCGG[AT]
BsmBI         320  GAGACG               CGTCTC(1/5)
BsmI         2230  GAATGC               GAATGC(1/-1)
Bsp1407I      834  TGTACA               T^GTACA
BspDI          17  ATCGAT               AT^CGAT
BspMI         864  ACCTGC               ACCTGC(4/8)
BsrBI        5334  CCGCTC               CCGCTC(-3/-3)
BsrFI        5218  ACCGGT               [GA]^CCGG[CT]
BsrGI         834  TGTACA               T^GTACA
Bst1107I     5415  GTATAC               GTA^TAC
BstUI          64  CGCG                 CG^CG
BstZ17I      5415  GTATAC               GTA^TAC
CelII        2721  GCTAAGC              GC^T[ACGT]AGC
Cfr10I       5218  ACCGGT               [GA]^CCGG[CT]
ClaI           17  ATCGAT               AT^CGAT
DraI         6073  TTTAAA               TTT^AAA
DraIII       2108  CACCTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I      1537  ATGCAT               ATGCA^T
Hin1I        4212  GACGCC               G[GA]^CG[CT]C
MfeI         3926  CAATTG               C^AATTG
MunI         3926  CAATTG               C^AATTG
MvnI           64  CGCG                 CG^CG
NdeI          659  CATATG               CA^TATG
NsiI         1537  ATGCAT               ATGCA^T
PaeR7I         58  CTCGAG               C^TCGAG
PflFI        3192  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        5218  ACCGGT               A^CCGGT
SacI         5749  GAGCTC               GAGCT^C
SexAI        6066  ACCAGGT              A^CC[AT]GGT
SgrAI        5217  CACCGGTG             C[GA]^CCGG[CT]G
SspBI         834  TGTACA               T^GTACA
Tth111I      3192  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          192  TCTAGA               T^CTAGA
XhoI           58  CTCGAG               C^TCGAG
XmnI         5532  GAAGTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

BbvCI         433  CCTCAGC              CCTCAGC(-5/-2)
             3781  CCTCAGC             
BciVI        1737  GGATAC               GTATCC(6/5)
             1796  GTATCC              
BglI         4281  GCCTCGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             4352  GCCCCTTGGGC         
BglII        1179  AGATCT               A^GATCT
             2541  AGATCT              
BsaI           47  GAGACC               GGTCTC(1/5)
             2983  GAGACC              
BspHI        1470  TCATGA               T^CATGA
             5768  TCATGA              
BspLU11I      397  ACATGT               A^CATGT
              837  ACATGT              
BsrDI        5900  CATTGC               GCAATG(2/0)
             3454  GCAATG              
BstEII       3334  GGTGACC              G^GT[ACGT]ACC
             3988  GGTGACC             
BstPI        3334  GGTGACC              G^GT[ACGT]ACC
             3988  GGTGACC             
CfoI           63  GCGC                 GCG^C
             4986  GCGC                
EcoO65I      3334  GGTGACC              G^GT[ACGT]ACC
             3988  GGTGACC             
HhaI           63  GCGC                 GCG^C
             4986  GCGC                
HinP1I         63  GCGC                 G^CGC
             4986  GCGC                
HincII        999  GTCAAC               GT[CT]^[GA]AC
             3356  GTCAAC              
HindII        999  GTCAAC               GT[CT]^[GA]AC
             3356  GTCAAC              
HindIII      2330  AAGCTT               A^AGCTT
             2778  AAGCTT              
RcaI         1470  TCATGA               T^CATGA
             5768  TCATGA              
SapI         3919  GAAGAGC              GCTCTTC(1/4)
             5239  GAAGAGC             
StuI         3521  AGGCCT               AGG^CCT
             4242  AGGCCT              
XcmI         4500  CCAGGCTGCTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             5315  CCAGTGACAAGTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       AocI        ApoI        AspHI       
AvaI        AvaII       BalI        BanII       BbsI        
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrI        BssKI       BssSI       BstAPI      
BstF5I      BstNI       BstXI       BstYI       Bsu36I      
Cac8I       Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        Eco81I      EcoNI       
EcoO109I    EcoRI       EcoRII      EcoRV       EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScaI        ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII