##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII BbeI BbrPI BcgI BclI
BfrI BsaAI BsaBI BseAI BsiEI
BsiWI BspEI BssHII BstBI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I FbaI FseI FspI HaeII
HpaI KasI KpnI KspI MamI
MluI MroI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfoI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 5415 GTATAC GT^[AC][GT]AC
AccII 64 CGCG CG^CG
AcyI 4212 GACGCC G[GA]^CG[CT]C
AgeI 5218 ACCGGT A^CCGGT
Asp700I 5532 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3192 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 10 CCTAGG C^CTAGG
BamHI 3252 GGATCC G^GATCC
BanI 2879 GGCACC G^G[CT][GA]CC
BlnI 10 CCTAGG C^CTAGG
BlpI 2721 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2721 GCTAAGC GC^T[ACGT]AGC
BsaHI 4212 GACGCC G[GA]^CG[CT]C
BsaWI 5218 ACCGGT [AT]^CCGG[AT]
BsmBI 320 GAGACG CGTCTC(1/5)
BsmI 2230 GAATGC GAATGC(1/-1)
Bsp1407I 834 TGTACA T^GTACA
BspDI 17 ATCGAT AT^CGAT
BspMI 864 ACCTGC ACCTGC(4/8)
BsrBI 5334 CCGCTC CCGCTC(-3/-3)
BsrFI 5218 ACCGGT [GA]^CCGG[CT]
BsrGI 834 TGTACA T^GTACA
Bst1107I 5415 GTATAC GTA^TAC
BstUI 64 CGCG CG^CG
BstZ17I 5415 GTATAC GTA^TAC
CelII 2721 GCTAAGC GC^T[ACGT]AGC
Cfr10I 5218 ACCGGT [GA]^CCGG[CT]
ClaI 17 ATCGAT AT^CGAT
DraI 6073 TTTAAA TTT^AAA
DraIII 2108 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 1537 ATGCAT ATGCA^T
Hin1I 4212 GACGCC G[GA]^CG[CT]C
MfeI 3926 CAATTG C^AATTG
MunI 3926 CAATTG C^AATTG
MvnI 64 CGCG CG^CG
NdeI 659 CATATG CA^TATG
NsiI 1537 ATGCAT ATGCA^T
PaeR7I 58 CTCGAG C^TCGAG
PflFI 3192 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 5218 ACCGGT A^CCGGT
SacI 5749 GAGCTC GAGCT^C
SexAI 6066 ACCAGGT A^CC[AT]GGT
SgrAI 5217 CACCGGTG C[GA]^CCGG[CT]G
SspBI 834 TGTACA T^GTACA
Tth111I 3192 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 192 TCTAGA T^CTAGA
XhoI 58 CTCGAG C^TCGAG
XmnI 5532 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BbvCI 433 CCTCAGC CCTCAGC(-5/-2)
3781 CCTCAGC
BciVI 1737 GGATAC GTATCC(6/5)
1796 GTATCC
BglI 4281 GCCTCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4352 GCCCCTTGGGC
BglII 1179 AGATCT A^GATCT
2541 AGATCT
BsaI 47 GAGACC GGTCTC(1/5)
2983 GAGACC
BspHI 1470 TCATGA T^CATGA
5768 TCATGA
BspLU11I 397 ACATGT A^CATGT
837 ACATGT
BsrDI 5900 CATTGC GCAATG(2/0)
3454 GCAATG
BstEII 3334 GGTGACC G^GT[ACGT]ACC
3988 GGTGACC
BstPI 3334 GGTGACC G^GT[ACGT]ACC
3988 GGTGACC
CfoI 63 GCGC GCG^C
4986 GCGC
EcoO65I 3334 GGTGACC G^GT[ACGT]ACC
3988 GGTGACC
HhaI 63 GCGC GCG^C
4986 GCGC
HinP1I 63 GCGC G^CGC
4986 GCGC
HincII 999 GTCAAC GT[CT]^[GA]AC
3356 GTCAAC
HindII 999 GTCAAC GT[CT]^[GA]AC
3356 GTCAAC
HindIII 2330 AAGCTT A^AGCTT
2778 AAGCTT
RcaI 1470 TCATGA T^CATGA
5768 TCATGA
SapI 3919 GAAGAGC GCTCTTC(1/4)
5239 GAAGAGC
StuI 3521 AGGCCT AGG^CCT
4242 AGGCCT
XcmI 4500 CCAGGCTGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5315 CCAGTGACAAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BalI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BssSI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRI EcoRII EcoRV EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII