##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AclI AcyI AgeI AhdI
Aor51HI AscI AspEI AvrII BbeI
BlnI BsaHI BsiEI BsiWI BsmBI
BspDI BsrFI BssHII BstBI Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I FseI HaeII
Hin1I HpaI KasI KspI MfeI
MunI NaeI NarI NcoI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmeI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SnaBI Sse8387I XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 8153 GGTACC G^GTACC
AccI 9335 GTATAC GT^[AC][GT]AC
AccIII 9 TCCGGA T^CCGGA
AflII 5069 CTTAAG C^TTAAG
AseI 9271 ATTAAT AT^TAAT
AsnI 9271 ATTAAT AT^TAAT
Asp700I 4426 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 8153 GGTACC G^GTACC
AspI 2636 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2737 TGCGCA TGC^GCA
BamHI 1326 GGATCC G^GATCC
BbrPI 3105 CACGTG CAC^GTG
BcgI 9234 GCAAAAACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1255 TGATCA T^GATCA
BfrI 5069 CTTAAG C^TTAAG
BseAI 9 TCCGGA T^CCGGA
Bsp1407I 9924 TGTACA T^GTACA
BspEI 9 TCCGGA T^CCGGA
BsrBI 5899 CCGCTC CCGCTC(-3/-3)
BsrGI 9924 TGTACA T^GTACA
BssSI 8147 CTCGTG CACGAG(-5/-1)
Bst1107I 9335 GTATAC GTA^TAC
BstEII 2545 GGTGACC G^GT[ACGT]ACC
BstPI 2545 GGTGACC G^GT[ACGT]ACC
BstZ17I 9335 GTATAC GTA^TAC
DsaI 7267 CCGTGG C^C[GA][CT]GG
EcoO65I 2545 GGTGACC G^GT[ACGT]ACC
EcoRV 547 GATATC GAT^ATC
FbaI 1255 TGATCA T^GATCA
FspI 2737 TGCGCA TGC^GCA
HgaI 6 GCGTC GACGC(5/10)
HindIII 1214 AAGCTT A^AGCTT
KpnI 8153 GGTACC GGTAC^C
MluI 4 ACGCGT A^CGCGT
MroI 9 TCCGGA T^CCGGA
NdeI 2439 CATATG CA^TATG
PflFI 2636 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3105 CACGTG CAC^GTG
PmlI 3105 CACGTG CAC^GTG
PshAI 5852 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 9271 ATTAAT AT^TAAT
SacI 8178 GAGCTC GAGCT^C
SmaI 7531 CCCGGG CCC^GGG
SpeI 4183 ACTAGT A^CTAGT
SphI 1132 GCATGC GCATG^C
SspBI 9924 TGTACA T^GTACA
SwaI 5765 ATTTAAAT ATTT^AAAT
Tth111I 2636 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 7531 CCCGGG C^CCGGG
XmaI 7531 CCCGGG C^CCGGG
XmnI 4426 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
ApaI 9199 GGGCCC GGGCC^C
9837 GGGCCC
AvaI 7531 CCCGGG C^[CT]CG[GA]G
8049 CCCGAG
BbvCI 3552 CCTCAGC CCTCAGC(-5/-2)
7604 GCTGAGG
BlpI 2493 GCTAAGC GC^T[ACGT]AGC
9673 GCTAAGC
Bpu1102I 2493 GCTAAGC GC^T[ACGT]AGC
9673 GCTAAGC
BsaWI 9 TCCGGA [AT]^CCGG[AT]
2840 TCCGGT
BsoBI 7531 CCCGGG C^[CT]CG[GA]G
8049 CCCGAG
BspHI 4691 TCATGA T^CATGA
5016 TCATGA
BstXI 8568 CCAGCTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
9189 CCAAAGCAGTGG
CelII 2493 GCTAAGC GC^T[ACGT]AGC
9673 GCTAAGC
EcoT22I 5081 ATGCAT ATGCA^T
8361 ATGCAT
NsiI 5081 ATGCAT ATGCA^T
8361 ATGCAT
RcaI 4691 TCATGA T^CATGA
5016 TCATGA
ScaI 3489 AGTACT AGT^ACT
9814 AGTACT
SexAI 5407 ACCTGGT A^CC[AT]GGT
8011 ACCTGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI AspHI AvaII BalI
BanI BanII BbsI BbvI BciVI
BcnI BfaI BglI BglII BmyI
BpmI BpuAI BsaAI BsaBI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspLU11I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DraIII EaeI
EarI Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NheI NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XcmI XhoII