################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AatII AclI AcyI AgeI AhdI Aor51HI AscI AspEI AvrII BbeI BlnI BsaHI BsiEI BsiWI BsmBI BspDI BsrFI BssHII BstBI Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I FseI HaeII Hin1I HpaI KasI KspI MfeI MunI NaeI NarI NcoI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmeI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI Sse8387I XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 8153 GGTACC G^GTACC AccI 9335 GTATAC GT^[AC][GT]AC AccIII 9 TCCGGA T^CCGGA AflII 5069 CTTAAG C^TTAAG AseI 9271 ATTAAT AT^TAAT AsnI 9271 ATTAAT AT^TAAT Asp700I 4426 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 8153 GGTACC G^GTACC AspI 2636 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2737 TGCGCA TGC^GCA BamHI 1326 GGATCC G^GATCC BbrPI 3105 CACGTG CAC^GTG BcgI 9234 GCAAAAACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1255 TGATCA T^GATCA BfrI 5069 CTTAAG C^TTAAG BseAI 9 TCCGGA T^CCGGA Bsp1407I 9924 TGTACA T^GTACA BspEI 9 TCCGGA T^CCGGA BsrBI 5899 CCGCTC CCGCTC(-3/-3) BsrGI 9924 TGTACA T^GTACA BssSI 8147 CTCGTG CACGAG(-5/-1) Bst1107I 9335 GTATAC GTA^TAC BstEII 2545 GGTGACC G^GT[ACGT]ACC BstPI 2545 GGTGACC G^GT[ACGT]ACC BstZ17I 9335 GTATAC GTA^TAC DsaI 7267 CCGTGG C^C[GA][CT]GG EcoO65I 2545 GGTGACC G^GT[ACGT]ACC EcoRV 547 GATATC GAT^ATC FbaI 1255 TGATCA T^GATCA FspI 2737 TGCGCA TGC^GCA HgaI 6 GCGTC GACGC(5/10) HindIII 1214 AAGCTT A^AGCTT KpnI 8153 GGTACC GGTAC^C MluI 4 ACGCGT A^CGCGT MroI 9 TCCGGA T^CCGGA NdeI 2439 CATATG CA^TATG PflFI 2636 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3105 CACGTG CAC^GTG PmlI 3105 CACGTG CAC^GTG PshAI 5852 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 9271 ATTAAT AT^TAAT SacI 8178 GAGCTC GAGCT^C SmaI 7531 CCCGGG CCC^GGG SpeI 4183 ACTAGT A^CTAGT SphI 1132 GCATGC GCATG^C SspBI 9924 TGTACA T^GTACA SwaI 5765 ATTTAAAT ATTT^AAAT Tth111I 2636 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 7531 CCCGGG C^CCGGG XmaI 7531 CCCGGG C^CCGGG XmnI 4426 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### ApaI 9199 GGGCCC GGGCC^C 9837 GGGCCC AvaI 7531 CCCGGG C^[CT]CG[GA]G 8049 CCCGAG BbvCI 3552 CCTCAGC CCTCAGC(-5/-2) 7604 GCTGAGG BlpI 2493 GCTAAGC GC^T[ACGT]AGC 9673 GCTAAGC Bpu1102I 2493 GCTAAGC GC^T[ACGT]AGC 9673 GCTAAGC BsaWI 9 TCCGGA [AT]^CCGG[AT] 2840 TCCGGT BsoBI 7531 CCCGGG C^[CT]CG[GA]G 8049 CCCGAG BspHI 4691 TCATGA T^CATGA 5016 TCATGA BstXI 8568 CCAGCTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 9189 CCAAAGCAGTGG CelII 2493 GCTAAGC GC^T[ACGT]AGC 9673 GCTAAGC EcoT22I 5081 ATGCAT ATGCA^T 8361 ATGCAT NsiI 5081 ATGCAT ATGCA^T 8361 ATGCAT RcaI 4691 TCATGA T^CATGA 5016 TCATGA ScaI 3489 AGTACT AGT^ACT 9814 AGTACT SexAI 5407 ACCTGGT A^CC[AT]GGT 8011 ACCTGGT ######################################### # 3 OR MORE CUT ENZYMES ( 132 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaLI ApoI AspHI AvaII BalI BanI BanII BbsI BbvI BciVI BcnI BfaI BglI BglII BmyI BpmI BpuAI BsaAI BsaBI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspLU11I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NheI NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoII